Abstract
Since the biologic behavior and molecular genetic changes observed in testicular germ cell cancer differ from those seen in more common epithelial tumors, it is likely that hitherto uncharacterized genes play a role in the development of germ cell tumors. Our previous work on testicular germ cell cancer suggested that chromosome 5q might contain one or more novel tumor suppressor genes that play a role in this malignancy. In this study, we performed a high resolution loss of heterozygosity (LOH) study of testicular cancer using 37 informative markers on chromosome 5. We detected allelic losses in 20/48 (42%) specimens and identified three common sites of loss on chromosome 5q14, 5q21 and 5q34-qter, defined respectively by minimal regions of deletion of ⩽1 cM, 10 cM and ∼20 (cM). Using an overlapping series of YACs and radiation hybrid mapping, we have constructed a physical map of the 5q14 deletion that should aid in the isolation and characterization of the putative tumor suppressor gene located therein.
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References
al-Jehani RM, Povey S, Delhanty JD and Parrington JM. . 1995 Genes Chromosomes Cancer 13: 249–256.
Aoki T, Mori T, Du X, Nisihira T, Mastubara T and Nakamura Y. . 1994 Genes Chromosome Cancer 10: 177–182.
Asada Y, Varnum DS, Frankel WN and Nadeau JH. . 1994 Nat. Genet. 6: 363–368.
Bosl GJ and Motzer RJ. . 1997 N. Engl. J. Med. 337: 242–253.
Canzian F, Salovaara R, Hemminki A, Kristo P, Chadwick RB, Aaltonen LA and de la Chapelle A. . 1996 Cancer Res. 56: 3331–3337.
Ding SF, Delhanty JD, Dooley JS, Bowles L, Wood CB and Habib NA. . 1993 Cancer Detect. Prev. 17: 405–409.
Fairman J, Chumakov I, Chinault AC, Nowell PC and Nagarajan L. . 1995 Proc. Natl. Acad. Sci. USA 92: 7406–7410.
Greenwald BD, Harpaz N, Yin J, Huang Y, Tong Y, Brown VL, McDaniel T, Newkirt C, Resau JH and Meltzer SJ. . 1992 Cancer Res. 52: 741–745.
Hagemeijer A and Grosveld G. . 1996 In: Leukemia. Henderson ES, Lister TA, Greaves MF (eds). WB Saunders: Philadelphia, USA pp. 131–144.
Hatta Y, Hirama T, Takeuchi S, Lee E, Pham E, Miller CW, Strohmeyer T, Wilczynski SP, Melmed S and Koeffler HP. . 1995 J. Urol. 154: 1954–1957.
Hosoe S, Ueno K, Shigedo Y, Tachibana I, Osaki T, Kumagai T, Tanio Y, Kawase I, Nakamura Y and Kishimoto T. . 1994 Cancer Res. 54: 1787–1790.
Huddart RA, Wooster R, Horwich A and Cooper CS. . 1995 Br. J. Cancer 72: 642–645.
Hudson TJ, Stein LD, Gerety SS, Ma J, Castle AB, Silva J, Slonim DK, Baptista R, Kruglyak L, Xu SH, et al. 1995 Science 270: 1945–1954.
International Testicular Cancer Linkage Consortium . 1998 Apmis 106: 64–72.
King BL, Peng HQ, Goss P, Huan S, Bronson D, Kacinski BM and Hogg D. . 1997 Cancer Res. 57: 209–214.
Leahy MG, Tonks S, Moses JH, Brett AR, Huddart R, Forman D, Oliver RT, Bishop DT and Bodmer JG. . 1995 Hum. Mol. Genet. 4: 1551–1555.
Lothe RA, Peltomaki P, Tommerup N, Fossa SD, Stenwig AE, Borresen AL and Nesland JM. . 1995 Lab. Invest. 73: 606–614.
Miura I, Graziano SL, Cheng JQ, Doyle LA and Testa JR. . 1992 Cancer Res. 52: 1322–1328.
Murty VV, Bosl GJ, Houldsworth J, Meyers M, Mukherjee AB, Reuter V and Chaganti RS. . 1994 Oncogene 9: 2245–2251.
Murty VV, Reuter VE, Bosl GJ and Chaganti RS. . 1996 Oncogene 12: 2719–2723.
Nagarajan L. . 1995 Leuk. Lymphoma 17: 361–366.
Nicholson PW and Harland SJ. . 1995 Br. J. Cancer 71: 421–426.
Noguchi T and Noguchi M. . 1985 J. Natl. Cancer. Inst. 75: 385–392.
Ozai A, Neiman RS, Ulbright TM, Heerema NA, John K and Nichols CR. . 1993 Cancer 71: 3873–3881.
Pletomaki P, Halme A and de la Chapelle A. . 1990 Cancer Genet. Cytogenet. 48: 1–12.
Peng HQ, Bailey D, Bronson D, Goss PE and Hogg D. . 1995 Cancer Res. 55: 2871–2875.
Peng HQ, Hogg D, Malkin D, Bailey D, Gallie BL, Bulbul M, Jewett M, Buchanan J and Goss PE. . 1993 Cancer Res. 53: 3574–3578.
Rodriguez E, Mathew S, Reuter V, Ilson DH, Bosl GJ and Chaganti RS. . 1992 Cancer Res. 52: 2285–2291.
Sakurai T, Katoh H, Moriwaki K, Noguchi T and Noguchi M. . 1994 Mann. Genome. 5: 333–336.
Scherer SW and Tsui L-C. . 1997 In: Advanced Techniques in Chromosome Analysis. Adolph KW (ed.).. Marcel Dekker: New York pp. 37–72.
Stewart EA, McKusick KB, Aggarwal A, Bajorek E, Brady S, Chu A, Fang N, Hadley D, Harris M, Hussain S, Lee R, Maratukulam A, K OC, Perkins S, Piercy M, Qin F, Reif T, Sanders C, She X, Sun WL, Tabar P, Voyticky S, Cowles S, Fan JB, Cox DR, et al. 1997 Genome Res. 7: 422–433.
Tamura G, Ogasawara S, Nishizuka S, Sakata K, Maesawa C, Suzuki Y, Terashima M, Saito K and Satodate R. . 1996 Cancer Res. 56: 612–615.
Weber JL and May PE. . 1989 Am. J. Hum. Genet. 44: 388–396.
Wieland I, Bohm M, Arden KC, Ammermuller T, Bogatz S, Viars CS and Rajewsky MF. . 1996 Oncogene 12: 97–102.
Acknowledgements
We would like to acknowledge the kind assistance of Dr Michael Jewett, of the Department of Surgery at The Toronto Hospital who supplied many of the testicular cancer specimens. We also thank Dr Steven Scherer of the Medical Research Council Genome Resource Facility at the Hospital for Sick Children, Toronto, for supplying the YAC clones. Finally, we gratefully acknowledge the patients who consented to provide material for this study. This work was supported by a Terry Fox Grant from the National Cancer Institute of Canada to D Hogg.
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Peng, HQ., Liu, L., Goss, P. et al. Chromosomal deletions occur in restricted regions of 5q in testicular germ cell cancer. Oncogene 18, 3277–3283 (1999). https://doi.org/10.1038/sj.onc.1202662
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DOI: https://doi.org/10.1038/sj.onc.1202662