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Aneuploidy of human testicular germ cell tumors is associated with amplification of centrosomes

Oncogene volume 22, pages 3859–3866 (2003)Cite this article

Abstract

Testicular germ cell tumors occur in three age groups. Seminomas and nonseminomas of adults, including mature teratomas, and the precursor carcinoma in situ (CIS) are aneuploid. This also holds true for yolk sac tumors of newborn and infants, while the mature teratomas of this age are diploid. In contrast, spermatocytic seminomas occurring in the elderly contain both diploid and polyploid cells. Aneuploidy has been associated with centrosome aberrations, sometimes related to overexpression of STK15. Aneuploidy of non-neoplastic germ cells has been demonstrated in the context of male infertility, a risk factor for the development of seminoma/nonseminoma. We investigated aneuploidy, centrosome aberrations and the role of STK15 in different types of testicular germ cell tumors as well as in normal and disturbed spermatogenesis. The aneuploid seminomas and nonseminomas tumors (including CIS) showed increased numbers of centrosomes, without STK15 amplification or overexpression. Four out of six infantile teratomas had normal centrosomes, the remaining two and an infantile yolk sac tumor showed a heterogeneous pattern of cells with normal or amplified centrosomes. Spermatocytic seminomas had two, four or eight centrosomes. Germ cells in seminiferous tubules with disturbed spermatogenesis shared both aneuploidy and centrosome abnormalities with seminomas/nonseminomas and showed a more intense STK15 staining than those with normal spermatogenesis and CIS. Therefore, aneuploidy of testicular germ cell tumors is associated with amplified centrosomes probably unrelated to STK15.

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Acknowledgements

We thank Wim van Putten (Department of Statistics, Erasmus MC, Rotterdam) for supporting the statistical analysis, and Dr Jeffrey L Salisbury (Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, USA) for the anticentrin antibody.

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Authors and Affiliations

  1. Pathology/Laboratory for Experimental Patho-Oncology, Josephine Nefkens Institute, Erasmus Medical Center/Daniel den Hoed Cancer Center, Rotterdam, 3000 DR, The Netherlands

    Frank Mayer, Hans Stoop, J Wolter Oosterhuis & Leendert H J Looijenga

  2. Abteilung für Onkologie, Hämatologie, Immunologie und Rheumatologie, Medizinische Universitaetsklinik Tübingen, Tübingen, 72076, Germany

    Frank Mayer & Carsten Bokemeyer

  3. Division of Pathology & Laboratory Medicine, Box 54, Room Y4.5609, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, 77030, TX, USA

    Subrata Sen

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Correspondence to Leendert H J Looijenga.

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Mayer, F., Stoop, H., Sen, S. et al. Aneuploidy of human testicular germ cell tumors is associated with amplification of centrosomes. Oncogene 22, 3859–3866 (2003). https://doi.org/10.1038/sj.onc.1206469

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