Abstract
It has been shown that acetylcholinesterase (AChE) expression was induced during apoptosis and the anti-sense oligonucleotides and siRNA of AChE may prevent apoptosis in various cell types. However, the mechanisms underlying AChE upregulation remain elusive. We demonstrated here that c-Jun NH2-terminal kinase (JNK) could mediate AChE expression. In this study, both etoposide and excisanin A, two anticancer agents, induced apoptosis in colon cancer cell line SW620 as determined by Annexin V staining, the cleavage of caspase-3 and the proteolytic degradation of poly (ADP-ribose) polymerase (PARP). The results showed that both the agents upregulated AChE in SW620 cells. In the meantime, JNK was also activated and the expression and phosphorylation of c-Jun increased in SW620 cells exposed to the two agents. The induced AChE mRNA and protein expression could be blocked by SP600125, a specific inhibitor of SAPK/JNK, and small interfering RNA directed against JNK1/2. Transfection with adenovirus-mediated dominant negative c-Jun also blocked the upregulation of AChE expression. Together, these results suggest that AChE expression may be mediated by the activation of JNK pathway during apoptosis through a c-Jun-dependent mechanism.
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References
Chen YR, Tan TH . (2000). The c-Jun N-terminal kinase pathway and apoptotic signaling (review). Int J Oncol 16: 651–662.
Cohen TV, Randall WR . (2004). J Neurochem 90: 1059–1067.
Collett GP, Campbell FC . (2004). Carcinogenesis 25: 2183–2189.
Cuadrado A, Gonzalez L, Suarez Y, Martinez T, Munoz A . (2004). Oncogene 23: 4673–4680.
Eminel S, Klettner A, Roemer L, Herdegen T, Waetzig V . (2004). J Biol Chem 279: 55385–55392.
Essers MA, Weijzen S, de Vries-Smits AM, Saarloos I, de Ruiter ND, Bos JL et al. (2004). EMBO J 23: 4802–4812.
Gao YH, Cheng Y, Ye HC . (2000). Chin Tradit Herb Drugs 31: 645–646.
Getman DK, Mutero A, Inoue K, Taylor P . (1995). J Biol Chem 270: 23511–23519.
Grisaru D, Sternfeld M, Eldor A, Glick D, Soreq H . (1999). Eur J Biochem 264: 672–686.
Gururajan M, Chui R, Karuppannan AK, Ke J, Jennings CD, Bondada S . (2005). Blood 106: 1382–1391.
Hamdi M, Kool J, Cornelissen-Steijger P, Carlotti F, Popeijus HE, van der Burgt C et al. (2005). Oncogene 48: 7135–7144.
Karpel R, Ben Aziz-Aloya R, Sternfeld M, Ehrlich G, Ginzberg D, Tarroni P et al. (1994). Exp Cell Res 210: 268–277.
Khelifi AF, D'Alcontres MS, Salomoni P . (2005). Cell Death Differ 12: 724–733.
Kirkland RA, Franklin JL . (2003). Antioxid Redox Signal 5: 589–596.
Kops GJ, Dansen TB, Polderman PE, Saarloos I, Wirtz KW, Coffer PJ et al. (2002). Nature 419: 316–321.
Kuntzen C, Sonuc N, De Toni EN, Opelz C, Mucha SR, Gerbes AL et al. (2005). Cancer Res 65: 6780–6788.
Kurinna SM, Tsao CC, Nica AF, Jiffar T, Ruvolo PP . (2004). Cancer Res 64: 7852–7856.
Lei K, Davis RJ . (2003). Proc Natl Acad Sci USA 100: 2432–2437.
Lei K, Nimnual A, Zong WX, Kennedy NJ, Flavell RA, Thompson CB et al. (2002). Mol Cell Biol 22: 4929–4942.
Meshorer E, Bryk B, Toiber D, Cohen J, Podoly E, Dori A et al. (2005). Mol Psychiatry 10: 985–997.
Meshorer E, Toiber D, Zurel D, Sahly I, Dori A, Cagnano E et al. (2004). J Biol Chem 279: 29740–29751.
Park SE, Kim ND, Yoo YH . (2004). Cancer Res 64: 2652–2655.
Perry C, Sklan EH, Birikh K, Shapira M, Trejo L, Eldor A et al. (2002). Oncogene 21: 8428–8441.
Small DH, Michaelson S, Sberna G . (1996). Neurochem Int 28: 453–483.
Smeal T, Binetruy B, Mercola DA, Birrer M, Karin M . (1991). Nature 354: 494–496.
Teraishi F, Wu S, Zhang L, Guo W, Davis JJ, Dong F et al. (2005). Cancer Res 65: 6380–6387.
Tournier C, Hess P, Yang DD, Xu J, Turner TK, Nimnual A et al. (2000). Science 288: 870–874.
Tranum-Jensen J, Behnke O . (1981). Eur J Cell Biol 24: 281–286.
Yin D, Zhou H, Kumagai T, Liu G, Ong JM, Black KL et al. (2005). Oncogene 24: 344–354.
Zhang XJ, Yang L, Zhao Q, Caen JP, He HY, Jin QH et al. (2002). Cell Death Differ 9: 790–800.
Zhou JM, Zhu XF, Lu YJ, Deng R, Huang ZS, Mei YP et al. (2006). Oncogene 25: 503–511.
Zhu XF, Liu ZC, Xie BF, Li ZM, Feng GK, Xie HH et al. (2002). Life Sci 70: 1259–1269.
Zhu XF, Xie BF, Zhou JM, Feng GK, Liu ZC, Wei XY et al. (2005). Mol Pharmacol 67: 1444–1450.
Zou W, Liu X, Yue P, Zhou Z, Sporn MB, Lotan R et al. (2004). Cancer Res 64: 7570–7578.
Acknowledgements
We thank Professor You-Heng Gao from Guangzhou University of Traditional Chinese Medicine School of Pharmaceutical Science for providing excisanin A. This work was supported by the National Natural Science Foundation of China (No. 30472037, 30572363), 973 program (2004CB518801) and the Research Grants Committee of Hong Kong (HKUST 6120/02M, 6133/03M; AoE/B15/01).
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Deng, R., Li, W., Guan, Z. et al. Acetylcholinesterase expression mediated by c-Jun-NH2-terminal kinase pathway during anticancer drug-induced apoptosis. Oncogene 25, 7070–7077 (2006). https://doi.org/10.1038/sj.onc.1209686
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DOI: https://doi.org/10.1038/sj.onc.1209686
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