Abstract
WHEN sodium azide (5 × 10−3 M) was added as an inhibitor of nitro-reductase activity of a Nocardia sp. using p-dinitrobenzene as substrate we were unable to detect the formation of p-nitroaniline by means of a diazotization assay1 although the appearance of a yellow colour indicated its formation. However, after extracting the reaction mixture with ether it was possible to show electrophoretically the presence of p-nitroaniline in the ether extract and that sodium azide at the above concentration has no inhibitory effect on the nitro-reductase system.
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References
Glazco, A. J., Wolf, L. M., and Dill, W. A., Arch. Biochem., 23, 411 (1949).
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VILLANUEVA, J. Interference by Azide with Diazotization Procedures used in Biological Assay Systems. Nature 184, 549 (1959). https://doi.org/10.1038/184549a0
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DOI: https://doi.org/10.1038/184549a0
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