Abstract
IN studies of cellular metabolism, the potential advantages of using dispersed cell suspensions rather than tissue homogenates or slices have led a number of workers to investigate the methods of preparation and properties of cell suspensions from various tissues1–5. Liver parenchymal and kidney epithelial cell suspensions prepared by mechanical disruption of the tissue, with or without prior perfusion of the organ, appear to be morphologically intact when examined by either light or electron microscopy1,2. These cell suspensions, however, are apparently metabolically different from tissue slices, since they show negligible endogenous respiration which cannot be restored by glucose or a number of protein and non-protein factors2,3 and have lost the ability to convert intracellular carbohydrate to lactic acid3. The oxidation of succinate by these cells, however, has been reported to proceed at a rate exceeding that observed with slices2,3, and is independent of exogenous cytochrome c (ref. 4). Liver cell suspensions have also been found to incorporate glycine at a rate seven-fold greater than that observed with homogenates6.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Anderson, N. G., Science, 117, 627 (1953).
Kalant, H., and Young, F. G., Nature, 179, 816 (1957).
Laws, J. O., and Stickland, L. H., Nature, 178, 309 (1956).
Kaltenbach, J. P., Exp. Cell. Res., 7, 568 (1954).
Branster, M. V., and Morton, R. K., Nature, 180, 1283 (1957).
LePage, G. A., Cancer Res., 13, 178 (1953).
LePage, G. A., Cancer Res., 10, 77 (1950).
Dounce, A. L., Barnett, S. R., and Beyer, G. T., J. Biol. Chem., 185, 769 (1950).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
ZIMMERMAN, M., DEVLIN, T. & PRUSS, M. Anærobic Glycolysis of Dispersed Cell Suspensions from Normal and Malignant Tissues. Nature 185, 315–316 (1960). https://doi.org/10.1038/185315a0
Issue Date:
DOI: https://doi.org/10.1038/185315a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.