Abstract
CERTAIN problems have induced us to prepare the chromosomes of bovine leucocytes. Using the methods of other authors1–3 the following technique was adopted: 20.0–40.0 ml. blood were collected from the jugular vein of an ox, to which was added 2.0–4.0 ml. heparin solution (200 mg heparin, 100 mg streptomycin, 10,000 units penicillin, 100.0 ml. of a 0.85 per cent saline solution) or an appropriate amount of ‘Vetren’ or ‘Thrombovetren’ (Promonta, Hamburg). This was put in a vessel containing 0.12–0.24 ml. phytohæmagglutinin P or 0.4–0.8 ml. phytohæmagglutinin M (Difco), well mixed, stored for 20 min in a refrigerator, centrifuged afterwards for 10 min at about 900g and the leucocyte-layer obtained. (Bovine erythrocytes were not agglutinated by phytohæmagglutinin.)
Similar content being viewed by others
Article PDF
References
Edwards, J. H., and Young, R. B., Lancet, ii, 49 (1961).
Kosenow, W., and Pfeiffer, R. A., Blut, 7, 500 (1961).
Moorhead, P. S., Nowell, P. C., Mellman, W. J., Battips, D. M., and Hungerford, D. A., Exp. Cell Res., 20, 613 (1960).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
ULBRICH, F., WEINHOLD, E. & PFEIFFER, R. Preparation of Bovine Chromosomes. Nature 199, 719 (1963). https://doi.org/10.1038/199719a0
Issue Date:
DOI: https://doi.org/10.1038/199719a0
This article is cited by
-
Kälbercorneazellen in Kultur
Zeitschrift für Medizinische Mikrobiologie und Immunologie (1970)
-
Chromosome studies in the superfamily Bovoidea
Chromosoma (1968)
-
Blood Culture Method for the Study of Bovine Chromosomes
Nature (1964)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.