Abstract
WE have been concerned with the isolation of acid phosphatases (orthophosphoric monoester phosphohydrolases, 3.1.3.2.) in quantities sufficient for chemical studies of these enzymes. The sources chosen were: the potato, the human prostate gland and human seminal plasma. We have found that the potato is a poor source and, although the enzyme has been obtained in a form which is homogeneous to starch-gel electrophoresis and to ultracentrifugation, the method is tedious and, because of the very large purification factor involved (about 25,000), leads to a low final yield (about 10 mg from 50 kg of potatoes). The best preparation previously reported was apparently associated with a purification factor of about 1,000 (ref. 1).
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References
Andreu, M., Fernandez Alvarez, E., and Lora-Tamayo, M., Anales real soc. espan. fis y quim. (Madrid), 56B, 67 (1960).
Hudson, P. B., and London, M., Arch. Biochem. Biophys., 46, 141 (1953).
Boman, H. G., Arkiv Kemi, 12, 453 (1958).
Shulam, S., Mamrod, L., Gonder, M. J., and Soanes, W. A., J. Immun., 93, 474 (1964).
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VERNON, C., GAULDIE, J., HANSON, J. et al. Acid Phosphatases. Nature 208, 382–383 (1965). https://doi.org/10.1038/208382b0
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DOI: https://doi.org/10.1038/208382b0
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