Abstract
A PROCEDURE has been developed for the visualization of the glycolytic enzyme, phosphoglucose isomerase, after electrophoresis in a starch gel. The staining mixture applied to the sliced gel surface consists of fructose-6-phosphate (free of glucose-6-phosphate), glucose-6-phosphate dehydrogenase, NADP, phenazine methosulphate and the tetrazolium salt MTT. The formation of glucose-6-phosphate by the isomerase is coupled to the reduction of the tetrazolium dye, which turns blue.
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CARTER, N., PARR, C. Isoenzymes of Phosphoglucose Isomerase in Mice. Nature 216, 511 (1967). https://doi.org/10.1038/216511a0
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DOI: https://doi.org/10.1038/216511a0
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