Abstract
AFTER the discovery in 1949 of the protective action of glycerol against damage arising from freezing and thawing of spermatozoa1, many investigators have succeeded in freezing different cell types, for example, erythrocytes2,3 and lymphocytes, and cultured tissues with a considerable degree of survival. For certain cell types dimethyl sulphoxide (DMSO) acts as a better cryoprotective substance than glycerol4,5. It has not yet been possible, however, to obtain satisfactory results in the low-temperature preservation of myocardial cells. In a recent publication6 it was stated that 80 to 90 per cent of the myocardial cells in suspension were lysed during freezing; only 5 to 20 per cent of the surviving cells manifested spontaneous beating. We now describe a method for preserving myocardial cells at − 196° C, with a high degree of recovery after thawing.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Polge, C., Smith, A. U., and Parkes, A. S., Nature, 164, 666 (1949).
Smith, A. U., Lancet, ii, 910 (1950).
Krijnen, H. W., Kuivenhoven, A. C. J., and de Wit, J. J. F. M., Cryobiology, 5, 136 (1968).
Lovelock, J. E., and Bishop, M. W. H., Nature, 183, 1394 (1959).
Gross, W. O., Z. Naturforschung, 23b, 512 (1968).
Schöpf-Ebener, E., Gross, W. O., and Bucher, O. M., Cryobiology, 4, 200 (1968).
Harary, I., and Farley, B., Exp. Cell. Res., 29, 451 (1963).
Harary, I., and Slater, E. C., Biochim. Biophys. Act., 99, 227 (1965).
Kroon, A. M., and Jansen, R. J., Biochim. Biophys. Acta., 155, 629 (1968).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
OFFERIJNS, F., FREUD, G. & KRIJNEN, H. Reanimation of Myocardial Cells preserved in the Frozen State. Nature 222, 1174–1175 (1969). https://doi.org/10.1038/2221174a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/2221174a0
This article is cited by
-
Successful cryopreservation of auricle fragments from rat hearts at −196°C
Experientia (1978)
-
Reanimation of cultured mammalian myocardial cells during multiple cycles of trypsinization-freezing-thawing
In Vitro (1974)
-
Retention of Pacemaker Activity in Mammalian Myocardial Cells recovered from Liquid Nitrogen
Nature (1973)
-
Prolonged storage of mitochondria by freezing: Retention of respiratory control and energized swelling
Journal of Bioenergetics (1970)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.