Abstract
CORTICOSTERONE, the principal adrenal steroid in the rat,, is retained by cell nuclei in the brain of adrenalectomized rats by a process specific to the corticosterone structure1. Nuclear binding of hormone is greatest in the hippocampus, where we have previously found the highest tissue concentration of radioactive hormone, but is also significant throughout the rest of the brain1,2. The binding of steroid hormone to cell nuclei in other target tissues, indicates that the hormone receptor is protein which can be extracted from the nuclei by moderate salt concentrations3–6. We now present evidence that hormone within brain cell nuclei is bound to a macromolecular component, probably protein, which can be extracted from isolated nuclei by 0.4 M NaCl.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
McEwen, B. S., Weiss, J. M., and Schwartz, L. S., Brain Res., 16, 227 (1969).
McEwen, B. S., Weiss, J. M., and Schwartz, L. S., Brain Res., 17, 471 (1970).
King, R. J. B., and Gordon, J., J. Endocrinol., 39, 533 (1967).
Talwar, G. P., Sopori, M. L., Biswas, D. K., Segal, S. J., Biochem. J., 107, 765 (1968).
Puca, G. A., and Bresciani, F., Nature, 223, 745 (1969).
Mainwairing, W. I. P., J. Endocrinol., 44, 323 (1969).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. Biol. Chem., 193, 265 (1951).
Sephadex: Gel Filtration in Theory and Practice (Pharmacia, A. B., Uppsala, Sweden, 1966).
Jensen, E. V., Suzuki, T., Numata, M., Smith, S., and De Sombre, E. R., Steroids, 13, 417 (1969).
Hamilton, T. H., and Teng, C.-S., Genetics, 61 (Suppl.), 381 (1969).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
MCEWEN, B., PLAPINGER, L. Association of 3H Corticosterone-1,2 with Macromolecules extracted from Brain Cell Nuclei. Nature 226, 263–265 (1970). https://doi.org/10.1038/226263a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/226263a0
This article is cited by
-
Stress Effects on Neuronal Structure: Hippocampus, Amygdala, and Prefrontal Cortex
Neuropsychopharmacology (2016)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.