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Rapid reconstitution of dendritic cells after allogeneic transplantation of CD133+ selected hematopoietic stem cells

Leukemia volume 19, pages 161–165 (2005)Cite this article

TO THE EDITOR

Throughout the last decade, monoclonal antibodies against the CD34 antigen have been used to mark and select hematopoietic stem cells. In the meantime, a new antigen initially called AC-133 and now referred to as prominin-1 or CD133,1 a member of the family of pentaspan transmembrane glycoproteins, has been shown to be expressed on a subpopulation of CD34+ hematopoietic stem cells. CD133+ cells in the peripheral blood have been shown to comprise early stem cells with substantial NOD/SCID mouse repopulating capacity and potential plasticity.2 Large-scale immunomagnetic selection using a monoclonal anti-CD133 antibody allows to enrich sufficient numbers of hematopoietic progenitor cells to perform hematopoietic stem cell transplantation (HSCT). We performed a feasibility trial in 10 adult patients with leukemia to test whether sustained engraftment can be achieved with allogeneic CD133+ purified stem cells. Secondary end point was the kinetics of donor chimerism in myeloid and lymphoid cells as well as in blood dendritic cells (DCs).

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References

  1. Fargeas CA, Corbeil D, Huttner WB . AC133 antigen, CD133, prominin-1, prominin-2, etc.: prominin family gene products in need of a rational nomenclature. Stem Cells 2003; 21: 506–508.

    Article  CAS  Google Scholar 

  2. Kuci S, Wessels JT, Buhring HJ, Schilbach K, Schumm M, Seitz G et al. Identification of a novel class of human adherent CD34-stem cells that give rise to SCID-repopulating cells. Blood 2003; 101: 869–876.

    Article  CAS  Google Scholar 

  3. Richters CD, Hoekstra MJ, van Baare J, du Pont JS, Hoefsmit EC, Kamperdijk EW . Isolation and characterization of migratory human skin dendritic cells. Clin Exp Immunol 1994; 98: 330–336.

    Article  CAS  Google Scholar 

  4. Lang P, Bader P, Schumm M, Feuchtinger T, Einsele H, Fuhrer M et al. Transplantation of a combination of CD133+ and CD34+ selected progenitor cells from alternative donors. Br J Haematol 2004; 124: 72–79.

    Article  Google Scholar 

  5. Fernandez-Aviles F, Urbano-Ispizua A, Aymerich M, Colomer D, Rovira M, Martinez C et al. Serial quantification of lymphoid and myeloid mixed chimerism using multiplex PCR amplification of short tandem repeat-markers predicts graft rejection and relapse, respectively, after allogeneic transplantation of CD34+ selected cells from peripheral blood. Leukemia 2003; 17: 613–620.

    Article  CAS  Google Scholar 

  6. Auffermann-Gretzinger S, Lossos IS, Vayntrub TA, Leong W, Grumet FC, Blume KG et al. Rapid establishment of dendritic cell chimerism in allogeneic hematopoietic cell transplant recipients. Blood 2002; 99: 1442–1448.

    Article  CAS  Google Scholar 

  7. Reddy V, Iturraspe JA, Tzolas AC, Meier-Kriesche HU, Schold J, Wingard JR . Low dendritic cell count after allogeneic hematopoietic stem cell transplantation predicts relapse, death, and acute graft-versus-host disease. Blood 2004; 103: 4330–4335.

    Article  CAS  Google Scholar 

  8. Chklovskaia E, Nowbakht P, Nissen C, Gratwohl A, Bargetzi M, Wodnar-Filipowicz A . Reconstitution of dendritic and natural killer-cell subsets after allogeneic stem cell transplantation: effects of endogenous flt3 ligand. Blood 2004; 103: 3860–3868.

    Article  CAS  Google Scholar 

  9. Merad M, Hoffmann P, Ranheim E, Slaymaker S, Manz MG, Lira SA et al. Depletion of host Langerhans cells before transplantation of donor alloreactive T cells prevents skin graft-versus-host disease. Nat Med 2004; 10: 510–517.

    Article  CAS  Google Scholar 

  10. Bornhauser M, Thiede C, Brendel C, Geissler G, Oelschlagel U, Neubauer A et al. Stable engraftment after megadose blood stem cell transplantation across the HLA barrier: the case for natural killer cells as graft-facilitating cells. Transplantation 1999; 68: 87–88.

    Article  CAS  Google Scholar 

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Acknowledgements

We thank Kristina Hölig, Verona Schwarze, Anja Maiwald, Anja Weisske and Diana Döhler for PBSC preparation and CD133+ immunomagnetic selection. We also thank Manuela Neumann and Cornelia Grosse for cell sorting and chimerism analysis. This work was supported in part by the Deutsche Krebshilfe Grant 70-3197 (MB and CT) and by Miltenyi Biotec, Bergisch Gladbach, Germany.

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  1. Medizinische Klinik und Poliklinik I, University Hospital, Dresden, Germany

    M Bornhäuser, L Eger, U Oelschlaegel, S Auffermann-Gretzinger, A Kiani, J Schetelig, T Illmer, M Schaich, D Corbeil, C Thiede & G Ehninger

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  1. M Bornhäuser
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  2. L Eger
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Correspondence to M Bornhäuser.

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Bornhäuser, M., Eger, L., Oelschlaegel, U. et al. Rapid reconstitution of dendritic cells after allogeneic transplantation of CD133+ selected hematopoietic stem cells. Leukemia 19, 161–165 (2005). https://doi.org/10.1038/sj.leu.2403563

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