Abstract
THE nucleoside, 1-β-D-arabinofuranosylcytosine (ara-C) and the unrelated compound, hydroxyurea, are known to inhibit DNA replication in bacterial and animal cells1–3. Arabinofuranosyl cytidine-5′-triphosphate (ara-CTP) specifically interferes with the action of DNA polymerase II in bacterial cell extracts4, whereas hydroxyurea affects the ribonucleoside diphosphate reductase reaction5. We have shown that ara-C and hydroxyurea bring about transformation of hamster embryo cells in tissue culture6. Further, it has also been demonstrated that ara-C inhibits the incorporation of 3H-D-glucosamine (a precursor of N-acetyl-neuraminic acid) into glycoproteins and glycolipids of hamster embryo fibroblasts and transformed cells7. In continuing these experiments we have been interested in, first, determining the site or sites of action of ara-C (presumably as its triphosphate) in the cell and second, studying other inhibitors of DNA replication as to their possible effect on glycoprotein and glycolipid synthesis.
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HAWTREY, A., SCOTT-BURDEN, T. & ROBERTSON, G. Inhibition of glycoprotein and glycolipid synthesis in hamster embryo cells by cytosine arabinoside and hydroxyurea. Nature 252, 58–60 (1974). https://doi.org/10.1038/252058a0
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DOI: https://doi.org/10.1038/252058a0
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