Abstract
HOMOLOGOUS enzymes of species with different cell temperatures usually display temperature-adaptive differences in their catalytic properties1–7. Thus enzymes of low cell temperature species characteristically exhibit higher specific activities and lower activation free energies and enthalpies than the homologous enzymes of high cell temperature species2–7. For lactate dehydrogenase (LDH, E.G. 1.1.1.27; lactate: NAD+ oxidoreductase), a single M4 (muscle type) LDH molecule from a cold-water fish can catalyse the conversion of pyruvate to lactate three times as rapidly at 5 °C as an M4-LDH from a mammal5. In this paper we present evidence that these temperature-compensating differences in catalytic efficiency may derive in part from different amounts of exergonic hydration of the enzymes during the activation step in catalysis. Lowering the activation free energy (ΔG‡) ‘barriers’ to enzymic reactions by way of the reversible hydration of amino acid side chains and/or polar peptide linkages permits the adjustment of catalytic efficiencies without necessitating the modification of amino acid sequences in the active sites of the enzymes.
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References
Hochachka, P. W. & Somero, G. N. Strategies of Biochemical Adaptation Saunders, Philadelphia, 1973).
Somero, G. N. & Low, P. S. in Biochemical Adaptation to Environmental Change, Biochemical Society Symposium No. 41, (eds Smellie, R. M. S. & Pennock, J. F.) 33–42 (Biochemical Society, London, 1976).
Somero, G. N. J. exp. Zool. 194, 175–188 (1975).
Low, P. S. & Somero, G. N. J. exp. Zool. 198, 1–12 (1976).
Low, P. S., Bada, J. L. & Somero, G. N. Proc. natn. Acad. Sci. U.S.A. 70, 430–432 (1973).
Johnston, I. A. & Goldspink, G. Nature 257, 620–623 (1975).
Borgmann, U. & Moon, T. W. Can. J. Biochem. 53, 998–1004 (1975).
Taylor, S. S. & Oxley, S. S. Archs Biochem. Biophys. 175, 373–383 (1976).
Perham, R. N. Biochem. J. 111, 17–21 (1969).
Ting, S., Lai, C. Y. & Horecker, B. L. Archs Biochem. Biophys. 144, 476–484 (1971).
Low, P. S. & Somero, G. N. Proc. natn. Acad. Sci. U.S.A. 72, 3305–3309 (1975).
Koshland, D. E. & Neet, K. E. A. Rev. Biochem. 37, 359–410 (1968).
Low, P. S. & Somero, G. N. Proc. natn. Acad. Sci. U.S.A. 72, 3014–3018 (1975).
Johnson, F. H., Eyring, H. & Polissar, M. J. in Kinetic Basis of Molecular Biology, 1–41, (Wiley, New York, 1954).
Von Hippel, P. H. & Schleich, T. in Structure and Stability of Biological Macromolecules (eds Timesheff, S. N. & Fasman, G. D.) 417–574 (Dekker, New York, 1969).
Somero, G. N. & Low, P. S. Am. Nat. (in press).
Banerjee, S. K. & Rupley, J. A. J. biol. Chem. 248, 2117–2124 (1973).
Krishnan, C. V. & Friedman J. phys. Chem. 73, 1572–1580 (1969); 75, 3598–3606 (1971).
Pesce, A., Fondy, T. P., Stolzenbach, F., Castillo, F. & Kaplan, N. O. J. biol. Chem. 242, 2151–2167 (1967).
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SOMERO, G., LOW, P. Enzyme hydration may explain catalytic efficiency differences among lactate dehydrogenase homologues. Nature 266, 276–278 (1977). https://doi.org/10.1038/266276a0
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DOI: https://doi.org/10.1038/266276a0
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