Abstract
DNA complementary in sequence to the messenger RNA for pre-proparathyroid hormone was synthesised using reverse transcriptase. In a linked transcription–translation system using RNA polymerase and cell-free extract from wheat germ, the DNA directed the synthesis of a protein identified as pre-proparathyroid hormone by N-terminal Sequencing and by electrophoretic and immunologic criteria.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Potts, J. T. & Deftos, L. J. in Duncan's Diseases of Metabolism 2 (eds Bondy, P. K. & Rosenberg, L. E.) 1225–1430 (W. B. Saunders, Philadelphia, 1974).
Habener, J. F., Potts, J. T., Jr & Rich, A., J. biol. Chem. 2511, 3893–3899 (1976).
Jacobs, J. W., Kemper, B., Niall, H. D., Habener, J. F. & Potts, J. T. Jr, Nature 249, 155–157(1974).
Kemper, B., Habener, J. F., Ernst, M. D., Potts, J. T., Jr & Rich, A., Biochemistry 15, 15–19(1976).
Kemper, B., Habener, J. F., Potts, J. T., Jr & Rich, A., Biochemistry 15, 20–25 (1976).
Habener, J. F., Kemper, B., Rich, A. & Potts, J. T., Jr, Recent Prog. Hormone Res. 33, (in the press).
Kemper, B., Habener, J. F., Mulligan, R. C., Potts, J. T., Jr & Rich, A., Proc. natn. Acad. Sci. U.S.A. 71, 3731–3735 (1974).
Efstadiadis, A., Maniatis, T., Kafatos, F., Jeffrey, A. & Vournakis, J., Cell 4, 367–378 (1975).
Kacian, D. L. & Myers, J. C., Proc. natn. Acad. Sci. U.S.A. 73, 2191–2195 (1976).
Monahan, J. J., Harris, S. E., Woo, S. L., Robberson, D. L. & O'Malley, B. W., Biochemistry 15, 223–233 (1976).
Rozenblatt, S., Mulligan, R. C., Gorecki, M., Roberts, B. E. & Rich, A., Proc. natn. Acad. Sci. U.S.A. 73, 2747–2751 (1976).
Roberts, B. E., Gorecki, M., Mulligan, R. C., Danna, K. J., Rozenblatt, S. & Rich, A., Proc. natn. Acad. Sci. U.S.A. 72, 1922–1926 (1975).
Shine, J. & Dalgarno, L., Nature 254, 34–38 (1975).
Steitz, J. A. & Jakes, K., Proc. natn. Acad. Sci. U.S.A. 72, 4734–4738 (1975).
Ptashne, M. et al. Science 194, 156–161 (1976).
Dasgupta, R., Shih, D. S., Saris, C. & Kaesberg, P., Nature 256, 624–628 (1975).
Kemper, B., Nature 262, 321–323 (1976).
Rose, J. K. & Lodish, H. F., Nature 262, 32–37 (1976).
Maniatis, T., Kee, S. G., Efstratiadis, A. & Kafatos, F., Cell, 8 163–182 (1976).
Higuchi, R., Paddock, G. V., Wall, R. & Salser, W., Proc. natn. Acad. Sci. USA 73, 3146–3150 (1976).
Rabbitts, T. H., Nature 260, 221–225 (1976).
Rougeon, F., Kourilsky, P. & Mach, B., Nucleic Acids Res. 2, 2365–2378 (1975).
Anderson, C. W., Lewis, J. B., Atkins, J. F. & Gesteland, R. F., Proc. natn. Acad. Sci. U.S.A. 71, 2756–2760 (1974).
Storti, R. V., Coen, D. M. & Rich, A., Cell. 8, 251–528 (1976).
Laskey, R. A. & Mills, A. D., Eur. J. Biochem. 56, 335–341 (1975).
Segre, G. V., Habener, J. F., Powell, D., Tregear, G. W. & Potts, J. T. Jr, J. clin. Invest. 51, 3163–3172 (1972).
Laemmli, U. K., Nature 227, 680–685 (1970).
Maxam, A. M. & Gilbert, W., Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kronenberg, H., Roberts, B., Habener, J. et al. DNA complementary to parathyroid mRNA directs synthesis of pre-proparathyroid hormone in a linked transcription—translation system. Nature 267, 804–807 (1977). https://doi.org/10.1038/267804a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/267804a0
This article is cited by
-
The molecular biology of parathyroid disease
World Journal of Surgery (1991)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.