Abstract
CALCIUM ions play an important part in stimulus–secretion coupling1, and in particular have been implicated in the control of renin release from kidneys in vitro2. The calcium ionophores A23187 and X537A and its derivatives have been used in several studies of the effect of mobilisation and movement of calcium and other divalent ions on biological systems, including mast cells, pancreatic B cells and the neurohypophysis3–8. X537A and Br-X537A are of interest because they release calcium preferentially from intracellular sites irrespective of the extracellular calcium concentration8,9. In investigating calcium involvement in secretion and the action of ionophores, emphasis has been placed on in vitro studies, where the experimental conditions can be closely controlled. We report here the results of experiments on the effect of Br-X537A on the renin levels in Amphiuma means kidney in organ culture. This system has the advantages of an isolated tissue, which in addition retains its in vivo characteristics for extended periods. We show that Br-X537A has dose-dependent effects on both the synthesis and secretion of renin. These effects apparently represent two quite separate sites of action of the ionophore.
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References
Rubin, R. P. Calcium and the Secretory Process (Plenum, New York, 1974).
Saruta, T. & Matsuki, S. Endocrinol. Japon. 22, 137–140 (1975).
Scarpa, A., Baldassare, J. & Inesi, G. J. gen. Physiol. 60, 735–749 (1972).
Foreman, J. C., Mongar, J. L. & Gomperts, B. D. Nature 245, 249–251 (1973).
Pressman, B. C. Fedn Proc. 32, 1698–1703 (1973).
Pressman, B. C. & de Guzman, N. T. Ann. N. Y. Acad. Sci. 227, 380–391 (1974).
Somers, G., Devis, G. & Malaisse, W. J. FEBS Lett. 66, 20–22, (1976).
Nordman, J. J. & Dyball, R. E. J. Nature 225, 414–415 (1975).
Nordmann, J. J. & Currell, G. A. Nature 253, 646–647 (1975).
Oparil, S. & Haber, E. New Engl. J. Med. 291, 389–401, 446–457 (1974).
Veyrat, R., Brunner, H. R., Manning, E. L. & Muller, A. F. J. Urol. Nephrol. Paris 73, 271–275 (1967).
Romero, J. C. & Hoobier, S. W. Am. Heart J. 80, 701–708 (1970).
Zehr, J. E. & Feigl, E. O. Circulation Res. 32, Suppl. 1, 17–26 (1973).
Hofbauer, K. G., Zschiedrich, H., Hackenthal, E. & Gross, F. Circulation Res. 35, Suppl. 1, 193–201 (1974).
Weinberger, M. H., Aoi, W. & Henry, D. P. Circulation Res. 37, 318–324 (1975).
Sherwood, J. B., Robinson, S. H., Bassan, L. R., Rosen, S. & Gordon, A. S. Blood 40, 189–197 (1972).
Trowell, O. A. Expl Cell Res. 16, 118–147 (1959).
Bojeson, E. & Leyssac, P. P. Acta physiol. scand. 65, 20–32 (1965).
MacDougall, J. B. D. & Coupland, R. E. Expl Cell Res. 45, 385–398 (1967).
Monnickendam, M. A. & Balls, M. Comp. Biochem. Physiol. 50A, 359–363 (1975); J. Cell Sci. 11, 799–813 (1972).
Cohen, E. L. et al. J. Lab. clin. Med. 77, 1025–1038 (1971).
Haber, E., Koerner, T., Page, L. B., Kliman, B. & Purnode, A. J. clin. Endocr. Metab. 29, 1349–1355 (1969).
Chambers, E. L., Pressman, B. C. & Rose, B. Biochem. biophys. Res. Commun. 60, 126–132 (1974).
Fares, R. V. Endocrinology 89, 1057–1063 (1971).
Macleod, R. M. & Fontham, E. H. Endocrinology 86, 863–869 (1970).
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WORLEY, R., RICH, G. & PRYOR, J. Effect of calcium ionophore Br-X537A on renin synthesis and release in Amphiuma means kidney culture. Nature 271, 174–176 (1978). https://doi.org/10.1038/271174a0
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DOI: https://doi.org/10.1038/271174a0
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