Abstract
A cleavage map of the Moloney murine sarcoma viral DNA was constructed and compared with that of a spontaneously occurring deletion mutant. By restriction enzyme analysis, it was shown that a region encompassing over 40% of the viral information was not essential for transformation or rescue of the deletion mutant. The transforming region was further localised by analysis of the transforming activity in tissue culture of isolated restriction fragments of linear double-stranded sarcoma viral DNA. In each case, DNA fragments that retained transforming activity preserved the cell-derived insertion sequences of the viral genome. Moreover, such transformants invariably expressed RNA specific to this region. By these two approaches, it was possible to demonstrate that the transforming region of the viral genome begins very near or within the cell-derived insertion sequences. Thus, the transforming gene of this mammalian sarcoma virus originates from within the mouse cell genome.
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References
Aaronson, S. A., Bassin, R. H. & Weaver, C. J. Virol. 9, 701–794 (1972).
Benveniste, R. E. & Scolnick, E. M. Virology 51, 370–381 (1973).
Frankel, A. E., Neubauer, R. L. & Fischinger, P. J. J. Virol. 18, 481–490 (1976).
Anderson, G. R. & Robbins, K. C. J. Virol. 17, 335–361 (1976).
Scolnick, E. M., Goldberg, R. J. & Parks, W. P. Cold Spring Harb. Symp. quant. Biol. 39, 885–895 (1974).
Hu, S., Davidson, N. & Verma, I. M. Cell. 10, 469–477 (1977).
Shih, T. Y., Young, H. A., Coffin, J. M. & Scolnick, E. M., J. Virol. 25, 238–252 (1978).
Dina, D. Proc. natn. Acad. Sci. U.S.A. 75, 2694–2698 (1978).
Canaani, E., Duesberg, P. & Dina, D. Proc. natn. Acad. Sci. U.S.A. 74, 29–33 (1977).
Ball, J. K., McCarter, J. H. & Sunderland, S. M. Virology 56, 268–284 (1973).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
Graham, F. L. & van der Eb, A. J. Virology 52, 456–461 (1973).
Maisel, J., Klement, V., Lai, M. C., Ostertag, W. & Duesberg, P. Proc. natn. Acad. Sci. U.S.A. 70, 3536–3540 (1973).
Smotkin, D., Gianni, A. M., Rozenblatt, S. & Weinberg, R. A. Proc. natn. Acad. Sci. U.S.A. 72, 4910–4913 (1975).
Frankel, A. E. & Fischinger, P. J. Proc. natn. Acad. Sci. U.S.A. 73, 3705–3709 (1976).
Huebner, R. J., Hartley, J. W., Rowe, W. P., Lane, W. T. & Capps, W. I. Proc. natn. Acad. Sci. U.S.A. 56, 1164–1169 (1966).
Aaronson, S. A. & Rowe, W. P. Virology 42, 9–19 (1970).
Bassin, R. H., Tuttle, N. & Fischinger, P. J. Int. J. Cancer 6, 95–107 (1970).
Barbacid, M., Stephenson, J. R. & Aaronson, S. A. Nature 262, 554–559 (1976).
Parks, W. P., Howk, R. S., Anisowicz, A. & Scolnick, E. M. J. Virol. 18, 491–503 (1976).
Wang, L. H. et al. Proc. natn. Acad. Sci. U.S.A. 73, 3952–3956 (1976).
Andersson, P., Goldfarb, M. P. & Weinberg, R. A. Cell 16, 63–75 (1979).
Mellon, P. & Duesberg, P. H. Nature 270, 631–634 (1977).
Rothenberg, E., Donoghue, D. J. & Baltimore, D. Cell 13, 435–451 (1978).
Fritsch, E. & Temin, H. M. J. Virol. 21, 119–130 (1977).
Rowe, W. P., Pugh, W. E. & Hartley, J. W. Virology 42, 1136–1139 (1970).
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. J. biol. Chem. 193, 262–275 (1951).
Lowy, D. R., Rands, E. & Scolnick, E. M. J. Virol. 26, 291–298 (1978).
Cory, S. & Adams, J.M. Cell 11, 795–805 (1977).
Sanger, F. et al. Nature 265, 687–695 (1977).
Botchan, M., Topp, W. & Sambrook, J. Cell 9, 269–278 (1976).
Taylor, J. M., Illmensee, R. & Summers, J. Biochim. biophys. Acta 442, 324–330 (1976).
Stephenson, J. R. & Aaronson, S. A. J. exp. Med. 136, 175–184 (1972).
Scherrer, K. in Fundamental Techniques in Virology (eds Hobel, K. &Salzman, N. P.) 413–432 (Academic, New York, 1969).
Britten, R. J., Graham, D. E. & Neufeld, B. R. Meth. Enzym. 29, 363–418 (1974).
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Canaani, E., Robbins, K. & Aaronson, S. The transforming gene of Moloney murine sarcoma virus. Nature 282, 378–383 (1979). https://doi.org/10.1038/282378a0
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DOI: https://doi.org/10.1038/282378a0
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