Abstract
Glicentin is a 100-amino acid polypeptide purified from the porcine intestine1 and containing the immunodeterminants of glucagon2. We have previously reported3 the presence of glicentin immunoreactivity in the glucagon-containing, specific secretory granules (α granules) of the pancreatic and gastric A cell. With an improved immunocytochemical probe, the protein A–gold (pAg) technique4, we are now able to show that glucagon and glicentin-like material are topologically segregated in the a granule of the human pancreatic A cell.
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References
Sundby, F., Jacobsen, H. & Moody, A. J. Horm. Metab. Res. 8, 366–371 (1976).
Jacobsen, H., Demandt, A., Moody, A. J. & Sundby, F. Biochim. biophys. Acta 493, 452–459 (1977).
Ravazzola, M. et al. Endocrinology 105, 499–508 (1979).
Roth, J., Bendayan, M. & Orci, L. J. Histochem. Cytochem. 26, 1074–1081 (1978).
Like, A. A. Lab. Invest. 16, 937–951 (1967).
Sternberger, L. A. in Electron Microscopy of Enzymes, Principles and Methods (ed. Hayat, M. A.) 150–181 (Van Nostrand, New York, 1973).
Bussolati, G., Capella, C., Vassallo, G. & Solcia, E. Diabetologia 7, 181–188 (1971).
Grimelius, L. et al. Virchows Arch. B Cell Path. 20, 217–288 (1976).
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Ravazzola, M., Orci, L. Glucagon and glicentin immunoreactivity are topologically segregated in the α granule of the human pancreatic A cell. Nature 284, 66–67 (1980). https://doi.org/10.1038/284066a0
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DOI: https://doi.org/10.1038/284066a0
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