Abstract
The bending locus of trypanosome kinetoplast DNA, identified by gel electrophoresis, has tracts of a simple repeat sequence (CA5–6 T) symmetrically distributed about it, with a repeat interval of 10 base pairs. The analogous bending induced when catabolite gene activating protein binds to its recognition sequence near the promoter of the Escherichia coli lac operon is centred on a site about 5–7 base pairs away from the centre of the protein binding site.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
McGhee, J. D. & Felsenfeld, G. A. Rev. Biochem. 49, 1115–1156 (1980).
Klevan, L. & Wang, J. C. Biochemistry 19, 5229–5234 (1980).
Better, M., Lu, C., Williams, R. C. & Echols, H. Proc. natn. Acad. Sci. U.S.A. 79, 5837–5841 (1982).
Ohlendorf, D. H., Andersen, W. F., Fisher, R. G., Takeda, Y. & Matthews, B. W. Nature 298, 718–723 (1982).
Wu, H. M., Dattagupta, N. & Crothers, D. M. Proc. natn. Acad. Sci. U.S.A. 80 (in the press).
Marini, J., Levene, S., Crothers, D. M. & Englund, P. T. Proc. natn. Acad. Sci. U.S.A. 79, 7664–7668 (1982).
Selsing, E. & Wells, R. J. biol. Chem. 254, 5410–5416 (1979).
Selsing, E., Well, R., Alden, C. & Arnott, S. J. biol. Chem. 254, 5417–5422 (1979).
Arnott, S. et al. Cold Spring Harb. Symp. quant. Biol. 47, 53–65 (1983).
de Crombrugghe, B. & Pastan, I. in The Operon (eds Miller, J. H. & Reznikoff, W. S.) 303–324 (Cold Spring Harbor Laboratory, New York, 1978).
Ullmann, A. & Danchin, A. Adv. Cyclic Nucleotide Res. 15, 1–53 (1983).
Majors, J. Nature 256, 672–674 (1975).
Gilbert, W. in RNA Polymerase (eds Losick, R. & Chamberlin, M.) 193–205 (Cold Spring Harbor Laboratory, New York, 1976).
Simpson, R. B. Nucleic Acid Res. 8, 759–766 (1980).
Schmilz, A. Nucleic Acid Res. 9, 277–292 (1981).
Caruthers, M. H. et al. Cold Spring Harb. Symp. quant. Biol. 47, 411–418 (1983).
Dickson, R. C., Abelson, J. N., Barnes, W. M. & Reznikoff, W. S. Science 182, 27–31 (1975).
Nakanishi, S., Adhya, S., Gottesman, M. & Pastan, I. J. biol. Chem. 254, 4050–4056 (1974).
Nakanishi, S., Adhya, S., Gottesman, M. & Pastan, I. J. biol. Chem. 250, 8202–8208 (1975).
Wells, R. D. et al. Proc. Nucleic Acid Res. molec. Biol. 24, 167–267 (1980).
McKay, D. B. & Steitz, T. A. Nature 290, 744–749 (1981).
Ebright, R. H. & Wong, J. R. Proc. natn. Acad. Sci. U.S.A. 78, 4011–4015 (1981).
Taniguchi, T., O'Neill, M. & de Crombrugghe, B. Proc. natn. Acad. Sci. U.S.A. 76, 5090–5094 (1979).
Lee, N. L., Gielow, W. O. & Wallace, R. G. Proc. natn. Acad. Sci. U.S.A. 78, 752–756 (1981).
Valentin-Hansen, P. EMBO J. 1, 1049–1054 (1982).
Kolb, A. & Buc, H. Nucleic Acids Res. 10, 473–485 (1982).
Unger, B., Clore, M., Gronenborn, A. & Hillen, W. EMBO J. 2, 289–293 (1983).
Cooper, T. B. in The Tools of Biochemistry 194–253 (Wiley, New York, 1977).
Lerman, L. S. & Frisch, H. L. Biopolymers 21, 995–997 (1982).
Lumpkin, O. J. & Zimm, B. H. Biopolymers 21, 2315–2316 (1982).
Peck, L. J. & Wang, J. C. Nature 292, 375–378 (1981).
Rhodes, D. & Klug, A. Nature 286, 573–578 (1980).
Simpson, R. T. & Kunzler, P. Nucleic Acids Res. 6, 1387–1415 (1979).
Rhodes, D. Nucleic Acids Res. 6, 1805–1816 (1979).
Kunkel, G. R. & Martinson, H. G. Nucleic Acids Res. 9, 6869–6888 (1981).
Garner, M. M. & Revzin, A. Biochemistry 24, 6032–6036 (1982).
Fried, M. G. & Crothers, D. M. Nucleic Acid Res. 11, 141–158 (1983).
Wartell, R., Larson, J. & Wells, R. J. biol. Chem. 249, 6719–6731 (1974).
Arnott, S., Chandrasekaran, R., Hail, I. & Puigjaner, L. Nucleic Acid Res. 11, 4141–4155 (1983).
Holmes, D. S. & Quigley, M. Analyt. Biochem. 114, 193–197 (1981).
Wu, H. M. thesis, Yale Univ. (1982).
Levene, S. & Crothers, D. M. J. biomolecular Structure Dynamics 1, 429–435 (1983).
Flory, P. J. Statistical Mechanics of Chain Molecules (Wiley, New York, 1969).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wu, HM., Crothers, D. The locus of sequence-directed and protein-induced DNA bending. Nature 308, 509–513 (1984). https://doi.org/10.1038/308509a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/308509a0
This article is cited by
-
Deciphering the mechanical code of the genome and epigenome
Nature Structural & Molecular Biology (2022)
-
Supercoiling and looping promote DNA base accessibility and coordination among distant sites
Nature Communications (2021)
-
Evaluating the role of coherent delocalized phonon-like modes in DNA cyclization
Scientific Reports (2017)
-
Protein-induced DNA linking number change by sequence-specific DNA binding proteins and its biological effects
Biophysical Reviews (2016)
-
Protein-induced DNA linking number change by sequence-specific DNA binding proteins and its biological effects
Biophysical Reviews (2016)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.