Abstract
A point mutation in the simian virus 40 large-T gene, which was generated by mixed oligonucleotide mutagenesis and resulted in the conversion of Lys 128 to Thr, produced a large-T antigen that was detected in the cytoplasm but not the nucleus of cells. Deletions within the surrounding sequence Lys-128Lys-Lys-Arg-Lys-Val-Glu also produce cytoplasmic large-T and define a region of the protein involved in nuclear location.
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Kalderon, D., Richardson, W., Markham, A. et al. Sequence requirements for nuclear location of simian virus 40 large-T antigen. Nature 311, 33–38 (1984). https://doi.org/10.1038/311033a0
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DOI: https://doi.org/10.1038/311033a0
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