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Abrogation of IL-3 and IL-2 dependence by recombinant murine retroviruses expressing v-myc oncogenes

Nature volume 317, pages 434–438 (1985)Cite this article

Abstract

Several oncogenes are thought to cause transformation by affecting the signal transmission pathway of growth factors. One example is the induction of c-myc, the cellular homologue of the avian transforming oncogene v-myc, by platelet-derived growth factor (PDGF)among a set of genes associated with competence induction in fibroblasts1,2. Another of the competence genes, r-fos, has been shown to be related to v-fos, the transforming gene of the FBJ sarcoma virus3. In addition, PDGF induces c-fos, the cellular homologue of v-fos4–6. The importance of c-myc induction is suggested by the observation that c-myc, under the control of a glucocorticoid regulator, can partially relieve the requirement of fibroblasts for PDGF7. We have examined the effects of oncogenes on haematopoietic/lymphoid cell differentiation, immortalization and factor dependence for growth. Here we report the effects of recombinant murine retroviruses capable of expressing the avian v-myc. With interleukin-3 (IL-3)- or interleukin-2 (IL-2)-dependent cells, the viruses abrogated the requirement for growth factors and suppressed c-myc expression.

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Author information

Authors and Affiliations

  1. Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick, Maryland, 21701, USA

    U. R. Rapp & J. L. Cleveland

  2. Department of Molecular Biology and Biochemistry, University of California, Irvine, California, 92717, USA

    K. Brightman

  3. NCI-Frederick Cancer Research Facility, Litton Bionetics Inc., Basic Research Program, Frederick, Maryland, 21701, USA

    A. Scott & J. N. Ihle

Authors
  1. U. R. Rapp
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  2. J. L. Cleveland
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  4. A. Scott
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  5. J. N. Ihle
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Rapp, U., Cleveland, J., Brightman, K. et al. Abrogation of IL-3 and IL-2 dependence by recombinant murine retroviruses expressing v-myc oncogenes. Nature 317, 434–438 (1985). https://doi.org/10.1038/317434a0

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