Abstract
The molecular structure of the photosynthetic reaction centre from Rhodopseudomonas viridis has been elucidated using X-ray crystallographic analysis. The central part of the complex consists of two subunits, L and M, each of which forms five membrane-spanning helices. We present the first description of the high-resolution structure of an integral membrane protein.
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References
Thornber, J. P., Cogdell, R. J., Seftor, R. E. B. & Webster, G. D. Biochim. biophys. Acta 593, 60–75 (1980).
Prince, R. C., Leigh, J. S. & Dutton, L. Biochim. biophys. Acta 440, 622–636 (1976).
Feher, G. & Okamura, M. Y. in The Photosynthetic Bacteria (eds Clayton, R. K. & Sistrom, W. R.) 349–386 (Plenum, New York, 1978).
Okamura, M. Y., Feher, G. & Nelson, N. in Photosynthesis: Energy Conversion by Plants and Bacteria (ed. Govindjee) 195–272 (Academic, New York, 1982).
Parson, W. W. & Ke, B. in Photosynthesis: Energy Conversion by Plants and Bacteria (ed. Govindjee) 331–384 (Academic, New York, 1982).
Hoff, A. J. in Light Reaction Path of Photosynthesis (ed. Fong, F. K.) 80–151, 322–326 (Springer, Berlin, 1982).
Parson, W. W. A. Rev. Biophys. Bioengng. 11, 57–80 (1982).
Michel, H. J. molec. Biol. 158, 567–572 (1982).
Zinth, W., Kaiser, W. & Michel, H. Biochim. biophys. Acta 723, 128–131 (1983).
Deisenhofer, J., Epp, O., Miki, K., Huber, R. & Michel, H. J. molec. biol. 180, 385–398 (1984).
Norris, J. R., Uphaus, R. A., Crespi, H. L. & Katz, J. J. Proc. natn. Acad. Sci. U.S.A. 68, 625–628 (1971).
Holten, D., Windsor, M. W., Parson, W. W. & Thornber, J. P. Biochim. biophys. Acta 501, 112–126 (1978).
Shuvalov, V. A. & Asadov, A. A. Biochim. biophys. Acta 545, 296–308 (1979).
Shopes, R. J. & Wraight, C. A. Biochim. biophys. Acta 806, 348–356 (1985).
Wang, B. C. Lecture Notes for School on Direct Methods and Macromolecular Crystallography7 (Medical Foundation of Buffalo, Buffalo, New York, 1983).
Peters, J., Welte, W. & Drews, G. FEBS Lett. 171, 267–270 (1984).
Wiemken, V. & Bachofen, R. FEBS Lett. 166, 155–159 (1984).
Michel, H., Deisenhofer, J., Miki, K., Weyer, K. & Lottspeich, F. in Structure and Function of Membrane Proteins (eds Quagliariello, E. & Palmieri, F.) 191–197 (Eisevier, Amsterdam, 1983).
Jones, T. A. J. appl. Crystallogr. 11, 268–272 (1978).
Jones, T. A. & Liljas, L. Acta Crystallogr. A40, 50–57 (1984).
Michel, H., Weyer, K. A., Gruenberg, H. & Lottspeich, F. EMBO J. 4, 1667–1672 (1985).
Brunisholz, R. A., Wiemken, V., Suter, F. & Bachofen, R. Hoppe-Seyler's Z. Physiol. Chem. 365, 689–701 (1984).
Rossmann, M. G. & Argos, P. J. molec. Biol. 105, 75–95 (1976).
Paillotin, G., Vermeglio, A., Breton, J. Biochim. biophys. Acta 545, 249–264 (1979).
Breton, J. in Proc. Feldafing Workshop 23–25 March 1985 (ed. Michel-Beyerle, M. E.) (Springer, Berlin, in the press).
Matthews, B. W., Ferma, R. E., Bolognesi, M. C. & Schmid, M. F. J. molec. Biol. 131, 259–285 (1979).
Schmid, M. F., Tronrud, D. E. & Matthews, B. W. Chim. scripta 21, 67–69 (1983).
Bunker, G., Stern, E. A., Blankenship, R. E. & Parson, W. W. Biophys. J. 37, 539–551 (1982).
Eisenberger, P., Okamura, M. Y. & Feher, G. Biophys. J. 37, 523–538 (1982).
Gast, P., Michalski, T. J., Hunt, J. E., Norris, J. R. FEBS Lett. 179, 325–328 (1985).
Pfister, K., Steinback, K. E., Gardner, G. & Arntzen, C. J. Proc. natn. Acad. Sci. U.S.A. 78, 981–985 (1981).
Stein, R. R., Castellvi, A. L., Bogacz, J. P. & Wraight, C. A. J. cell. Biochem. 24, 243–259 (1984).
Dismukes, G. C., Frank, H. A., Friesner, R. & Sauer, K. Biochim. biophys. Acta 764, 253–271 (1984).
Debus, R. J., Okamura, M. Y. & Feher, G. Biophys. J. 47, 3a (1985).
Hol, W. G. J. Prog. Biophys. molec. Biol. 45, 149–195 (1985).
Debus, R. J., Feher, G. & Okamura, M. Y. Biochemistry 24, 2488–2500 (1985).
Ploegman, J. H., Drent, G., Kalk, K. H. & Hol, W. G. J. J. molec. Biol. 123, 439–457 (1978).
Blundell, T. et al. Nature 289, 771–777 (1981).
Mathews, F. S. Prog. Biophys. molec. Biol. 45, 1–56 (1985).
Youvan, D. C., Bylina, E. J., Alberti, M., Begusch, H. & Hearst, J. E. Cell 37, 949–957 (1984).
Williams, J. C., Steiner, L. A., Ogden, R. C., Simon, M. I. & Feher, G. Proc. natn.Acad. Sci. U.S.A. 80, 6505–6509 (1983).
Williams, J. C., Steiner, L. A., Feher, G. & Simon, M. I. Proc. natn. Acad. Sci. U.S.A. 81, 7303–7307 (1984).
Zurawski, G., Bohnert, H. J., Whitfield, P. R. & Bottomley, W. Proc. natn. Acad. Sci. U.S.A. 79, 7699–7703 (1982).
Rasmussen, O. F., Bookjans, G., Stummann, B. M. & Henningsen, K. W. Pl. Molec. Biol. 3, 1984–1984 (1984).
Rochaix, J.-D., Dron, M., Rahire, M., Malnoe, P. Pl. molec. Biol 3, 363–370 (1984).
Alt, J., Morris, J., Westhoff, P. & Hermann, R. G. Curr. Genet. 8, 597–606 (1984).
Holschuh, K., Bottomley, W. & Whitfield, P. R. Nucleic Acids Res. 12, 8819–8834 (1984).
Michel, H., Deisenhofer, J. in Encyclopedia of Plant Physiology: Photosynthesis III Vol. 19 (eds Staehelin, A. C. & Arntzen, C. J.) (Springer, Berlin, in the press).
Rao, J. K. M., Hargrave, P. A. & Argos, P. FEBS Lett. 156, 165–169 (1983).
Lesk, A. M. & Hardman, K. D. Science 216, 539–540 (1982).
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Deisenhofer, J., Epp, O., Miki, K. et al. Structure of the protein subunits in the photosynthetic reaction centre of Rhodopseudomonas viridis at 3Å resolution. Nature 318, 618–624 (1985). https://doi.org/10.1038/318618a0
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DOI: https://doi.org/10.1038/318618a0
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