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References
Sambrook J, Fritsch EF, Maniatis T . Molecular Cloning: A Laboratory Manual, 2nd edn Cold Spring Harbor Laboratory: Cold Spring Harbor, NY 1989
Ioannou PA, et al. A new bacteriophage P1-derived vector for the propagation of large human DNA fragments Nat. Genet 1994 6: 84–89
Shizuya H, et al. Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor based vector Proc. Natl. Acad. Sci 1992 89: 8794–8797
Rothstein R . Targeting, disruption replacement and allele rescue: integrative DNA transformation in yeast Meth. Enzymol 1991 194: 281–301
Duff K, et al. Insertion of a pathogenic mutation in a yeast artificial chromosome containing the human amyloid precursor protein gene Gene Therapy 1994 1: 70–75
Vassaux G, Manson AL, Huxley C . Copy-number dependent expression of a YAC-cloned human CFTR gene in a human epithelial cell line Gene Therapy 1997 4: 618–623
Kouprina N et al. Functional copies of a human gene can be directly isolated by transformation-associated recombination cloning with a small 3’ end target sequence Proc. Natl. Acad. Sci. 1998 95: 4469–4474
Zhang Y, et al. A new logic for DNA engineering using recombination in Escherichia coli Nat. Genet 1998 20: 123–128
Narayanan K, et al. Efficient and precise engineering of a 200 kb β-globin human/bacterial artificial chromosome in E. coli DH10B using an inducible homologous recombination system Gene Therapy 1999 6: 442–447
Schiedner G, et al. Genomic DNA transfer with a high-capacity adenovirus vector results in improved in vivo gene expression and decreased toxicity Nat. Genet 1998 18: 180–183
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Vassaux, G. New cloning tools for the design of better transgenes. Gene Ther 6, 307–308 (1999). https://doi.org/10.1038/sj.gt.3300912
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DOI: https://doi.org/10.1038/sj.gt.3300912