Abstract
Rearrangement of the separated V, D and J segments of immunoglobulin heavy and light chain genes is an ordered and regulated process essential for the production and diversification of antibodies in mammals1. Only one allele of the heavy and light chain gene locus is found functionally rearranged in normal B cells. The second allele is either non-functionally ,or incompletely rearranged, a phenomenon known as allelic exclusion. Recently, we2 and others3 have shown that expression of a rearranged μ gene introduced into the germline of mice leads to inhibition of rearrangement of endogenous heavy chain genes. It has been suggested that μ also exerts a positive influence on kappa light chain gene rearrangement4. Here we show that a functionally rearranged δ gene is also able to prevent endogenous heavy chain gene rearrangement and could promote κ light chain gene rearrangement.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
1. Tonegawa, S. Nature 302, 575-581 (1983). 2. Rusconi, S. & Kohler, G. Nature 314, 330-334 (1985). 3. Weaver, D., Constantini, F., Imanishi-Kari, T. & Baltimore, D. Cell 42, 117-127 (1985). 4. Reth, M., Ammirati, P., Jackson, S. & Alt, F. Nature 317, 353-355 (1985). 5. Blattner, F. R. & Tucker, P. W. Nature 307, 417-422 (1984). 6. Shimizu, A. & Honjo, T. Cell 36, 801-803 (1984). 7. Radbruch, A. & Sablitzky, F. EMBO J. 2, 1929 (1983). 8. Coico, R. F. et al Nature 316, 744-746 (1985). 9. Palmiter, R. D. & Brinster, R. L. A. Rev. Genet. 20, 465-499 (1986). 10. Kohler, G. & Milstein, C. Eur. J. Immun. 6, 511-519 (1976). 11. Storb, U. et al. J. exp. Med. 164, 627-641 (1986). 12. Nussenzweig, M. C. et al. Science 236, 816-819 (1987). 13. Reth, M. G., Knieps, E., Wiese, P., Lobel, L. & Alt, F. W. EMBO J. (in the press). 14. Yuan, D. & Vitetta, E. S. / Immun. 120, 353-356 (1978). 15. Yuan, D., Gilian, A. C. & Tucker, P. W. Fen. Proc. 44, 2652-2659 (1985). 16. Oi, V. T., Jones, P. P., Coding, J. W. & Herzenberg, L. A. Curr. Top. microbiol. Immun. 81, 115 (1978). 17. Dialynas, D. P. et al. J. Immun. 131, 2445 (1983). 18. Yelton, D. E., Desaymard, C. & Scharff, M. D. Hybridoma 1, 5 (1981). 19. Kincade, P. W., Lee, G., Sun, L. & Watanabe, T. /. immunol. Meth. 42, 17-26 (1981). 20. Ledbetter, J. A. & Herzenberg, L. A. Immunol. Rev. 47, 63 (1979). 21. Mulligan, R. C. & Berg, P. Proc. natn. Acad. Sci. U.S.A. 78, 2072-2076 (1981). 22. Maki, R. et al. Cell 24, 353-365 (1981). 23. Alt, F. et al. EMBO J. 3, 1209-1219 (1984).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Iglesias, A., Lamers, M. & Köhler, G. Expression of immunoglobulin delta chain causes allelic exclusion in transgenic mice. Nature 330, 482–484 (1987). https://doi.org/10.1038/330482a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/330482a0
This article is cited by
-
Allelic Exclusion and Peripheral Reconstitution by TCR Transgenic T Cells Arising From Transduced Human Hematopoietic Stem/Progenitor Cells
Molecular Therapy (2013)
-
Immunoglobulin genes and diversity: what we have learned from domestic animals
Journal of Animal Science and Biotechnology (2012)
-
IgD can largely substitute for loss of IgM function in B cells
Nature (1998)
-
B-cell maturation in chimaeric mice deficient for the heat stable antigen (HSA/mouse CD24)
Transgenic Research (1995)
-
γ2b transgenic mice as a model for the role of immunoglobulins in B cell development
Immunologic Research (1994)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.