Abstract
Polioviruses occur as three distinct serotypes, 1, 2 and 3, and are composed of a single-stranded positive-sense RNA genome of ∼ 7,450 nucleotides enclosed in an icosahedral particle of diameter 27 nm. The three-dimensional crystallographic structure of poliovirus type 1 has been determined at 2.9 Å resolution, providing a detailed knowledge of the folding and arrangement of the individual virus proteins, VP1–VP41. From this and the charac-terization of monoclonal antibody-resistant mutants2–7, the amino acids contributing to antigenic sites have been identified and located on the surface of the virus particle. Here we describe the construction and characterization of a poliovirus chimaera having a defined region of type 3 inserted into type 1. This virus has composite antigenicity and the substitute site is immunogenic in small animals and primates. The ability to construct such viruses has implications for the design of improved poliovirus vaccine strains or vaccines against other picornaviruses, such as hepatitis A.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Hogle, J. M., Chow, M. & Filman, D. J. Science 229, 1358–1365 (1985).
Minor, P. D. et al. Nature 301, 674–679 (1983).
Minor, P. D. et al. J. gen. Virol. 66, 1159–1165 (1985).
Minor, P. D., Ferguson, M., Evans, D. M. A., Almond, J. W. & Icenogle, J. P. J. gen. Virol 67, 1283–1291 (1986).
Minor, P. D. et al. J. gen. Virol. 68, 1857–1865 (1987).
Diamond, D. et al. Science 229, 1090–1093 (1985).
Blondel, B. et al. J. Virol. 57, 81–90 (1986).
Sabin, A. B., Boulger, L. R. J. biol. Stand. 1, 115–118 (1973).
WHO Collaborative Study J. biol. Stand. 9, 163–184 (1981).
Omata, T. et al. J. Virol. 58, 348–358 (1986).
Westrop, G. D. et al. in The molecular biology of positive strand viruses (eds Rowlands, D. J., Mahy, B. W. J. & Mayo M.) 53–60 (Academic, London, 1986).
Westrop, G. D. et al. (in preparation).
Kramer, W., Drutsa, V., Jansen, H., Kramer, B., Pflugfelder, M. & Fritz, H. Nucleic Acids Res. 12, 9441–9456 (1984).
Evans, D. M. A., Minor, P. D., Schild, G. C. & Almond, J. W. Nature 304, 459–462 (1983).
Parker, B. A. & Stark, G. R. J. Virol. 31, 360–375 (1979).
Domok, I. & Magrath, D. I. WHO Offset Publication No. 46 (1979).
Ferguson, M. et al. J. gen. Virol. 65, 197–201 (1984).
Icenogle, J. P., Minor, P. D., Ferguson, M. & Hogle, J. M. J. Virol. 60, 297–301 (1986).
Minor, P. D., John, A., Ferguson, M. & Icenogle, J. P. J. gen. Virol. 67, 693–706 (1987).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Burke, K., Dunn, G., Ferguson, M. et al. Antigen chimaeras of poliovirus as potential new vaccines. Nature 332, 81–82 (1988). https://doi.org/10.1038/332081a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/332081a0
This article is cited by
-
Eukaryotic Virus Display: Engineering the Major Surface Glycoprotein of the Autographa californica Nuclear Polyhedrosis Virus (AcNPV) for the Presentation of Foreign Proteins on the Virus Surface
Bio/Technology (1995)
-
Cytokines as adjuvants in immunocompromised hosts
International Journal of Clinical and Laboratory Research (1995)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.