Abstract
Transcription factor IIB (TFIIB) has a central role in transcription of class II genes. The purification of the human TFIIB protein and isolation of a complementary DNA encoding TFIIB activity is reported here. The sequence of TFIIB, which seems to be encoded by a single gene, contains a repeated motif, in addition to a motif with similarity to the prokaryotic σ-factors. The recombinant protein expressed in bacteria substituted for all the functions attributed to the human TFIIB protein.
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Ha, I., Lane, W. & Reinberg, D. Cloning of a human gene encoding the general transcription initiation factor IIB. Nature 352, 689–695 (1991). https://doi.org/10.1038/352689a0
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DOI: https://doi.org/10.1038/352689a0
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