Abstract
ONE of the members of the bZIP family of transcriptional activators1–5 is NF-IL6/LAP (IL-6 DBF, C/EBPβ CRP2). NF-IL6/LAP protein is highly expressed in liver nuclei2, where it has been implicated as a master regulator of the acute-phase response1,3,6,7, induced by interleukin-6 (IL-6) and other inflammatory mediators3,8. Also, NF-IL6/LAP is involved in the activation of the IL-6 promoter in response to IL-1 and bacterial lipopolysaccharide1–6. The control of NF-IL6/LAP expression and activity is complex and poorly understood. Under some conditions the NF-IL6/LAP gene is transcriptionally activated by IL-1 and lipopolysaccharide1, whereas in other instances, its binding to cognate DNA sequences is enhanced by cytokines1,3. Additionally, the ability of constitutively expressed NF-IL6/LAP to activate transcription is strongly augmented by IL-6, through an unknown signalling pathway. We now show that stimulation of the protein kinase C pathway increases the phosphorylation of Ser 105 within the activation domain of NF-IL6/LAP, and enhances its transcriptional efficacy.
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Trautwein, C., Caelles, C., van der Geer, P. et al. Transactivation by NF-IL6/LAP is enhanced by phosphorylation of its activation domain. Nature 364, 544–547 (1993). https://doi.org/10.1038/364544a0
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DOI: https://doi.org/10.1038/364544a0
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