Abstract
OUR understanding of the regulation of vascular tone has been extended since the identification of vasoactive agents such as the atrial natriuretic peptides1, endothelial-derived relaxing factor2 and endothelin3. Unidentified vasopressive agents have been found in platelets4. Here we isolate these vasopressors and identify them as diadenosine pentaphosphate (AP5A) and diadenosine hex-aphosphate (AP6A) by chromatography, mass spectrometry, ultraviolet spectroscopy and enzymatic cleavage. In the vasculature of isolated perfused rat kidney, both diadenosine phosphates were active at a concentration of 10−9 M; in aortic rings, contractions were elicited at 10−8 M.Intra-aortic injection in the rat caused a prolonged increase in blood pressure. We conclude that AP5A and AP6A may play a part in local vasoregulation and possibly in the regulation of blood pressure.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Flynn, T. G., De Bold, M. L. & De Bold, A. J. Biochem. biophys. Res. Commun. 117, 859–865 (1983).
Palmer, R. M. J., Ferrige, A. G. & Moncada, S. Nature 327, 524–526 (1987).
Yanagisawa, M. et al. Nature 332, 411–415 (1988).
Agha, A. et al. J. vasc. Res. 29, 281–289 (1992).
Ally, A. & Park, G. J. Chromatogr. 575, 19–27 (1992).
Pintor, J., Torres, M. & Miras-Portugal, M. T. Life Sci. 48, 2317–2324 (1991).
Rodriguez del Castillo, A., Torres, M., Delicado, E. G. & Miras-Portugal, M. T. J. Neurochem. 51, 1696–1703 (1988).
Pintor, J., Rotllan, P., Torres, M. & Miras-Portugal, M. T. Analyt. Biochem. 200, 296–300 (1992).
Harrison, M. J. & Brossmer, R. FEBS Lett. 54, 57–60 (1975).
Morii, H. & Makinose, M. Eur. J. Biochem. 205, 979–984 (1992).
Pohl, U., Ogilvie, A., Lamontagne, D. & Busse, R. Am. J. Physiol. 260, H1692–H1697 (1991).
Nees, S. Eur. Heart J. 10, suppl. F 28–35 (1989).
Busse, R., Ogilvie, A. & Pohl, U. Am. J. Physiol. 254, H828–H832 (1988).
Baril, E. F., Coughlin, S. A. & Zamecnik, P. C. Cancer Invest. 3, 465–471 (1985).
Yamaguchi, N., Kodama, M. & Ueda, K. Gastroenterology 89, 723–731 (1985).
Weinmann-Dorsch, C. et al. Eur. J. Biochem. 138, 179–185 (1984).
Zamecnik, P. C., Stephenson, M. L., Janeway, C. M. & Randerath, K. Biochem. biophys. Res. Commun. 24, 91–97 (1966).
Frenz, J., Van der Schrieck, P. & Horvath, C. J. Chromatogr. 330, 1–17 (1985).
Zidek, W. et al. Clin. Exp. Hypertens. A12, 365–381 (1990).
Grynkiewicz, G., Poenie, M. & Tsien, R. Y. J. biol. Chem. 260, 3440–3450 (1985).
Neusser, M., Golinski, P., Zhu, Z., Tepel, M. & Zidek, W. J. vasc. Res. 30, 116–120 (1993).
Nordhoff, E. et al. Rapid Comm. Mass Spectrom. 6, 771–776 (1992).
Sulkowski, E. & Laskowski, M. Biochim. biophys. Acta 240, 443–447 (1971).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schlüter, H., Offers, E., Brüggemann, G. et al. Diadenosine phosphates and the physiological control of blood pressure. Nature 367, 186–188 (1994). https://doi.org/10.1038/367186a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/367186a0
This article is cited by
-
Migraine signaling pathways: purine metabolites that regulate migraine and predispose migraineurs to headache
Molecular and Cellular Biochemistry (2023)
-
Diadenosine pentaphosphate affects electrical activity in guinea pig atrium via activation of potassium acetylcholine-dependent inward rectifier
The Journal of Physiological Sciences (2017)
-
Mechanistic insights from resolving ligand-dependent kinetics of conformational changes at ATP-gated P2X1R ion channels
Scientific Reports (2016)
-
Diadenosine tetra- and pentaphosphates affect contractility and bioelectrical activity in the rat heart via P2 purinergic receptors
Naunyn-Schmiedeberg's Archives of Pharmacology (2016)
-
The enzymatic activity of the VEGFR2 receptor for the biosynthesis of dinucleoside polyphosphates
Journal of Molecular Medicine (2013)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.