Abstract
TRANSCRIPTION initiation factor TFIID is a multisubunit complex containing a TATA-box-binding factor (TFIIDτ/TBP) and associated polypeptide factors (TAFs) with sizes ranging from Mr ∼20,000 to >200,000 (refs 1–7). As a result of direct promoter interactions8,9, TFIID nucleates the assembly of RNA polymerase II and other initiation factors into a functional preinitiation complex10. Although the native TFIID complex mediates both basal and activator-dependent transcription in reconstituted systems, TBP itself is competent for only basal transcription. Thus, TAFs are essential cofactors for regulated transcription. The complementary DNAs encoding the p230 (Mr 230,000), p110 and p85 subunits of TFIID have recently been cloned11–18. Here we report the molecular cloning and characterization of the p62, p42, p28 and p22 subunits. These participate in a network of heterogeneous protein–protein interactions within TFIID. Sequence similarities between p62/p42 and the histones H4/H3, respectively, suggest that these subunits have a functional relationship with chromatin.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Dynlacht, B. D., Hoey, T. & Tjian, R. Cell 66, 563–576 (1991).
Tanese, N., Pugh, B. F. & Tjian, R. Genes Dev. 5, 2212–2224 (1991).
Takada, R. et al. Proc. natn. Acad. Scl. U.S.A. 89, 11809–11813 (1992).
Zhou, Q., Lieberman, P. M., Boyer, T. G. & Berk, A. J. Genes Dev. 6, 1964–1974 (1992).
Brou, C. et al. EMBO J. 12, 489–499 (1993).
Chiang, C.-M., Ge, H., Wang, Z., Hoffmann, A. & Roeder, R. G. EMBO J. 12, 2749–2762 (1993).
Kokubo, T. et al. J. biol. Chem. 268, 17554–17558 (1993).
Sawadogo, M. & Roeder, R. G. Cell 43, 165–175 (1985).
Nakajima, N., Horikoshi, M. & Roeder, R. G. Molec. cell. Biol. 8, 4028–4040 (1988).
Van Dyke, M. W., Roeder, R. G. & Sawadogo, M. Science 241, 1335–1338 (1988).
Kokubo, T. et al. Genes Dev. 7, 1033–1046 (1993).
Weinzierl, R. O. J., Dynlacht, B. D. & Tjian, R. Nature 362, 511–517 (1993).
Hisatake, K. et al. Nature 362, 179–181 (1993).
Ruppert, S., Wang, E. H. & Tjian, R. Nature 362, 175–179 (1993).
Kokubo, T., Gong, D.-W., Roeder, R. G., Horikoshi, M. & Nakatani, Y. Proc. natn. Acad. Sci. U.S.A. 90, 5896–5900 (1993).
Hoey, T. et al. Cell 72, 247–260 (1993).
Kokubo, T. et al. Molec. cell. Biol. 13, 7859–7863 (1993).
Dynlacht, B. D., Weinzierl, R. O. J., Admon, A. & Tjian, R. Nature 363, 176–179 (1993).
Struhl, K. Molec cell. Biol. 6, 3847–3853 (1986).
Gash, A., Hoffmann, A., Horikoshi, M. & Roeder, R. G. Nature 346, 390–394 (1990).
Horikoshi, M., Hai, T., Lin, Y. S., Green, M. R. & Roeder, R. G. Cell 54, 1033–1042 (1988).
Nakatani, Y. et al. Nature 348, 86–88 (1990).
Hayes, J. J., Clark, D. J. & Wolffe, A. P. Proc. natn. Acad. Sci. U.S.A. 88, 6829–6833 (1991).
Arents, G., Burlingame, R. W., Wang, B.-C., Love, W. E. & Moudrianakis, E. N. Proc. natn. Acad. Sci. U.S.A. 88, 10148–10152 (1991).
Wolffe, A. P. & Dimitrov, S. Crit. Rev. euk. Gene Exp. 3, 167–191 (1993).
Tabassum, R., Sandman, K. M. & Reeve, J. N. J. Bact. 174, 7890–7895 (1992).
Workman, J. L. & Roeder, R. G. Cell 51, 613–622 (1987).
Workman, J. L., Abmayr, S. M., Cromlish, W. A. & Roeder, R. G. Cell 55, 211–219 (1988).
Wooton, J. C. & Federhen, S. Comp. Chem. 17, 149–163 (1993).
Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. J. molec. Biol. 215, 403–410 (1990).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kokubo, T., Gong, DW., Wootton, J. et al. Molecular cloning of Drosophila TFIID subunits. Nature 367, 484–487 (1994). https://doi.org/10.1038/367484a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/367484a0
This article is cited by
-
Identification of the gene encoding the TATA box-binding protein-associated factor 1 (TAF1) and its putative role in the heat shock response in the protozoan parasite Entamoeba histolytica
Parasitology Research (2019)
-
Yeast two-hybrid map of Arabidopsis TFIID
Plant Molecular Biology (2007)
-
Asymmetry in the burial of hydrophobic residues along the histone chains of Eukarya, Archaea and a transcription factor
BMC Structural Biology (2005)
-
Molecular characterization of two newDrosophila melanogaster homologs of human TAFII30
Molecular Biology (2000)
-
The core curriculum
Nature (1996)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.