Abstract
Aim:
To investigate the cytotoxic effects of piperonal ciprofloxacin hydrazone (QNT4), a novel antibacterial fluoroquinolone derivative, against human hepatocarcinoma SMMC-7721 cells.
Methods:
Human hepatocarcinoma cells (SMMC-7721), human breast adenocarcinoma cells (MCF-7) and human colon adenocarcinoma cells (HCT-8) were tested. The effects of QNT4 on cell proliferation were examined using MTT assay. Cell apoptosis was determined using Hoechst 33258 fluorescence staining, TUNEL assay and agarose gel electrophoresis. The topoisomerase II activity was measured using agarose gel electrophoresis with the DNA plasmid pBR322 as the substrate. Mitochondrial membrane potential (Δψm) was measured using a high content screening imaging system. Protein expression of caspase-9, caspase-8, caspase-3, p53, Bcl-2, Bax, and cytochrome c was detected with Western blot analysis.
Results:
Treatment with QNT4 (0.625–10 μmol/L) potently inhibited the proliferation of the cancer cells in time- and dose-dependent manners (the IC50 value at 24 h in SMMC-7721 cells, MCF-7 cells and HCT-8 cells was 2.956±0.024, 3.710±0.027, and 3.694±0.030 μmol/L, respectively). Treatment of SMMC-7721 cells with QNT4 (0.2146, 2.964, and 4.600 μmol/L) for 24 h dose-dependently increased the percentage of apoptotic cells, elicited characteristic DNA “ladder” bands, and decreased the mitochondrial membrane potential. QNT4 dose-dependently increased topoisomerase II-mediated DNA breaks while inhibiting DNA relegation, thus keeping the DNA in fragments. Treatment of SMMC-7721 cells with QNT4 significantly increased cytochrome c in the cytosol, and decreased cytochrome c in the mitochondrial compartment. QNT4 (3–7.39 μmol/L) significantly increased the protein expression of p53, Bax, caspase-9, caspase-3, and the cleaved activated forms of caspase-9 and caspase-3 in SMMC-7721 cells. In contrast, the expression of Bcl-2 was decreased, while caspase-8 had no significant change.
Conclusion:
QNT4 induced the apoptosis of SMMC-7721 cells via inhibiting topoisomerase II activity and modulating mitochondrial-dependent pathways.
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Introduction
Antibacterial fluoroquinolone is a very important family of antibacterial drugs that are widely prescribed for the treatment of infections in humans1. According to the pharmacological mechanisms elucidated in numerous reports, antibacterial fluoroquinolone corrupts the activities of prokaryotic type II topoisomerase and DNA gyrase, and induces them to kill cells by generating high levels of DNA double-strand breaks. DNA gyrase modulates the topological state of the genetic material by passing an intact DNA helix through a transient double-strand break that is generated in a separate DNA segment2. Like bacterial cells, eukaryotic species also require a type II topoisomerase, known as topoisomerase II, for viability. Compared with the known sequences of type II topoisomerases of bacteria and mammals, the sequences around activated tyrosine residues appear to have common homology3. The mechanisms by which antitumor fluoroquinolones induce cell death appear to be similar to those of quinolone antibacterial agents4. Mammalian DNA topoisomerase II can be inhibited by fluoroquinolones, although it is 100-fold more sensitive to prokaryotic DNA gyrase. In addition, the mode of action of fluoroquinolones is similar to that of anthracycline derivatives (such as doxorubicin, amsacrine, mitoxantrone), epipodophyllotoxin derivatives (such as etoposide), and actinomycin D (a class of polypeptide antibiotics isolated from Streptomyces). Accordingly, antibacterial fluoroquinolones have been shown to be cytotoxic to cancer cells5, 6, thus representing a potentially important source of new anticancer agents. Recently, the development of antitumor agents derived from antibacterial fluoroquinolones has attracted much attention due to the mechanistic similarities and sequence homologies of the targeting eukaryotic topoisomerases7. However, many antitumor fluoroquinolones have been modified from clinical antibacterial fluoroquinolones with regard to the nitrogen-containing ring, such as piperazine, on the 7-position and (or) the 2-position of the fluoroquinolone scaffold8, 9. In addition, a few modifications of the carboxylic group at the 3-position have been reported10. Indeed, it does not seem necessary for an antitumor fluoroquinolone to retain the carboxylic group; fluoroquinolones with a fused heterocyclic ring as an isostere of the carboxylic group showed strong anticancer activity as well as high water solubility11. To search for new structural modification strategies for antibacterial fluoroquinolones, we have designed and synthesized a series of fluoroquinolone derivatives by linking various hydrazine compounds to the C-3 carboxyl group of ciprofloxacin or ofloxacin and assessed their anticancer activities. Several novel ciprofloxacin derivatives displayed potent cytotoxicity against the tested cancer cell lines in vitro, with IC50 values reaching micromolar concentrations12. In this study, we investigated the growth inhibitory effects and the molecular mechanisms of piperonal ciprofloxacin hydrazone (QNT4) in human hepatocarcinoma SMMC-7721 cells. We found that QNT4 (1-cyclopropyl-6-fluoro-4-oxo-7-piperazin-1, 4-dihydro-quinoline-3-carboxylic acid benzo[1,3]dioxol-5-ylmethylene-hydrazide) showed potent cytotoxicity against SMMC-7721 cells with an IC50 value of 2.411 μmol/L.
Materials and methods
Chemicals
Piperonal ciprofloxacin hydrazone (QNT4) was synthesized at the Institute of Chemistry and Biology at Henan University. The purity was >98% by HPLC analysis. The compound was dissolved in dimethyl sulfoxide (DMSO, Solarbio Science & Technology Co, Ltd). Its structure is illustrated in Figure 1.
Cell culture and treatments
Human hepatocarcinoma cells (SMMC-7721 and Hep3B2.1-7), human breast adenocarcinoma cells (MCF-7), and human colon adenocarcinoma cells (HCT-8), obtained from the Cell Bank of the Chinese Academy of Sciences, Shanghai, were cultured in DMEM medium (Gibco BRL, USA) supplemented with 10% heat-inactivated fetal bovine serum (Sijiqing Co, Ltd, Hangzhou, China), 100 IU/mL penicillin, and 100 μg/mL streptomycin. The cells were maintained in 5% CO2 at 37 °C. When the cells reached approximately 50%–70% confluence, they were treated with different amounts of chemicals as indicated. DMSO was used as the vehicle control.
MTT assay
The cells were seeded at a density of 1×104 cells/mL in 96-well culture plates. After 24 h, the cells were treated with the indicated concentrations of QNT4 or ciprofloxacin. Control wells consisted of cells incubated with medium only. After 24, 48, and 72 h of treatment, cells were incubated with 20 μL MTT (5 mg/mL, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide; Sigma, St Louis, MO, USA). After 4 h at 37 °C, the supernatant was removed, and 150 μL DMSO was added. After the blue crystals were dissolved in DMSO, the optical density (OD) was detected at a wavelength of 570 nm using a 96-well multiscanner autoreader (Bio-Rad, USA). The following formula was used: cell proliferation inhibited (%)=[1-(OD of the experimental samples/OD of the control)]×100. The IC50 is defined as the concentration at which cell proliferation is inhibited by 50%.
Hoechst 33258 staining
SMMC-7721 cells were seeded at a density of 1×104 cells/mL on the glass cover slides of a 35-mm chamber. After being treated with QNT4 for 24 h, the cells were washed twice with PBS and incubated with 5 μg/mL Hoechst 33258 (Sigma, St Louis, MO, USA) for 10 min at 37 °C in the dark. The cells were then washed and fixed with 4% paraformaldehyde in PBS for 5 min at 4 °C. Nuclear morphology was then examined under a fluorescent microscope (BX51, Olympus, Japan).
TUNEL assay
Cells (1×104 cells/mL) were seeded in growth medium on the glass cover slides of 6-well plates for overnight incubation. They were then treated with the indicated concentrations of QNT4 for 24 h. Control wells consisted of cells incubated with medium only. Next, cells were examined for apoptosis by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay (Promega, Madison, WI, USA), according to the manufacturer's instructions. Cells were visualized and photographed from at least five randomly chosen areas in each slide, using a fluorescent microscope (BX51, Olympus, Japan). Percent apoptosis was determined by counting the number of apoptotic cells and dividing by the total number of cells in the areas.
DNA agarose gel electrophoresis
Cells were treated with media containing different concentrations of QNT4 for 24 h and were then washed twice with PBS. The chromosomal DNA was extracted with the Apoptotic DNA Ladder Detection Kit (Beyotime, China) according to the manufacturer's instructions. The DNA sample was incubated at 37 °C for 30 min and electrophoresed at 40 V/cm on a 1% agarose gel containing 1 mg/mL ethidium bromide. Finally, the apoptotic DNA fragments were visualized under a UV transilluminator and photographed.
Topoisomerase II-mediated supercoiled pBR322 DNA relaxation assay
DNA topoisomerase II activity was determined by the supercoiled pBR322 DNA relaxation assay13. The experiments were performed by incubating human topoisomerase IIα (Sigma, St Louis, MO, USA) with 1 μg supercoiled pBR322 DNA in 5 μL relaxation buffer (200 mmol/L Tris-HCl, pH 7.5, 340 mmol/L KCl, 40 mmol/L MgCl2, 20 mmol/L DTT, 120 mg/L BSA, 5 mmol/L EDTA, 4 mmol/L ATP) under increasing concentrations of QNT4. In this experiment, etoposide (Sigma, St Louis, MO, USA), a known topoisomerase II poison14, was used as a positive control. The mixture was incubated at 37 °C for 30 min and the reaction was terminated by adding 20 μL 10% SDS and 1 μL protease K (1×104 mg/L). Samples were subjected to electrophoresis in 1% agarose gels. DNA was then stained with 1 mg/mL ethidium bromide and photographed under a UV transilluminator.
Estimate of mitochondrial membrane potential loss
Cells were treated with media containing different concentrations of QNT4 for 24 h and were then incubated with 0.5 mg/mL of the fluorescence probe JC-1 (5,5′,6,6′-tetrachloro-l, l′,3,3′- tetraethyl-benzimidazolcarbocyanine iodide, Beyotime, China) at 37 °C for 20 min. The cells were thoroughly washed twice with buffer and incubated with 5 μg/mL Hoechst 33258 for 10 min in the dark. After two additional washes, the mitochondrial membrane potential (Δψm) was measured by a high content screening imaging system (Thermo Fisher Scientific, USA).
Western blotting assay
After treatment with different concentrations of QNT4 for 24 h, cells were lysed with ice-cold RIPA lysis buffer. Protein concentrations were determined using the Bradford method. Samples were separated by 12% SDS–PAGE under reducing conditions and transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore). The membranes were blocked with 5% non-fat milk in TBST buffer (20 mmol/L Tris-HCl, 137 mmol/L NaCl, and 0.1% Tween 20, pH 8.0) for 1 h at room temperature, prior to an overnight incubation at 4 °C with specific antibodies to caspase-9, caspase-8, caspase-3, p53, Bcl-2, Bax, cytochrome c, or β-actin (all antibodies from Santa Cruz Biotechnology, USA). The membranes were washed and incubated with horseradish peroxidase conjugated anti-mouse IgG (Beijing ZhongShan GoldenBridge Biological Technology Co, LTD), or anti-rabbit IgG (Beyotime, China) secondary antibodies for 1 h. Once again, the membranes were washed three times with TBST, and the proteins were detected using an enhanced chemiluminescence substrate (ECL, Beyotime, China).
Cytochrome c release from mitochondria was evaluated by Western blotting analysis of cytosolic protein samples. Cytosolic protein fractions were prepared using the cell mitochondria isolation kit (Beyotime, China).
Statistical analyses
The data are presented as the mean±SD for the indicated number of independent experiments. Statistical significance was calculated using the t-test for paired samples. P<0.05 was regarded as significant and P<0.01 as highly significant.
Results
QNT4 suppressed the growth of cancer cells in vitro
The cytotoxicity of QNT4 against cancer cells was assessed using the MTT cell viability assay. The cells were treated with various concentrations of QNT4 for 24, 48, and 72 h, resulting in a significant decrease in cell viability in a dose- and time-dependent manner (Figure 2). Within the three cancer cell lines used in this experiment, QNT4 was most effective against SMMC-7721. As shown in Figure2A, the IC50 values after treatment for 24, 48, and 72 h were 2.956±0.024 μmol/L (r2=0.9521), 3.497±0.063 μmol/L (r2=0.8382), and 3.345±0.039 μmol/L (r2=0.9700), respectively. For MCF-7 cells, the IC50 values after treatment for 24, 48, and 72 h were 3.710±0.027 μmol/L (r2=0.8764), 3.377±0.021 μmol/L (r2=0.9438) and 4.299±0.029 μmol/L (r2=0.8857), respectively. For the HCT-8 cells, the IC50 values after treatment for 24, 48, and 72 h were 3.694±0.030 μmol/L (r2=0.8750), 4.568±0.018 μmol/L (r2=0.8330), and 4.318±0.025 μmol/L (r2=0.8096), respectively. In contrast, ciprofloxacin showed weak cytotoxicity against SMMC-7721 cells (Figure 2D). The IC50 value at 48 h treatments was 6.860±0.194 μmol/L (r2=0.8039).
QNT4-induced apoptosis of SMMC-7721 cells
After treatment with various concentrations of QNT4 for 24 h, marked morphological changes indicative of cell apoptosis, such as chromatin condensation and nuclear fragmentation, were clearly observed for SMMC-7721 cells following Hoechst 33258 staining (Figure 3, Table 1).
The integrity of DNA was assessed by agarose gel electrophoresis. As shown in Figure 4, 24 h incubation of SMMC-7721 cells with 2.964 μmol/L and 4.600 μmol/L QNT4 elicited characteristic DNA “ladder” bands indicative of apoptotic internucleosomal DNA fragmentation (approximately 180–200 bp).
To further investigate the role of QNT4 in apoptosis, TUNEL assay was performed. As shown in Figure 5 and Table 2, 24 h incubation with QNT4 increased the percentage of apoptotic SMMC-7721 cells in a concentration-dependent manner.
The effect of QNT4 on the catalytic activities of eukaryotic topoisomerase II
In this experiment, relative double-stranded DNA cleavage/religation was determined using agarose gel electrophoresis of treated-pBR322 DNA and nontreated-pBR322 DNA (Figure 6). QNT4 decreased the amount of supercoiled DNA (Form I), while increasing nicked circular plasmid molecules (Form II) and linear molecules (Form III) in a dose-dependent manner. The effects of QNT4 on topoisomerase II-mediated DNA cleavage/religation were similar to that of etoposide. This suggests that QNT4 increased topoisomerase II-mediated DNA breaks while inhibiting DNA religation.
The effect of QNT4 on the mitochondrial membrane potential
Mitochondrial membrane potential (Δψm) was detected with the fluorescent probe JC-1, which exists predominantly in monomeric form in cells with depolarized mitochondria and displays green fluorescence at 490 nm. In contrast, JC-1 primarily forms aggregates in cells with polarized mitochondria and shows reddish-orange fluorescence. The emission intensity ratio of the 545 nm and 595 nm peaks was used as a measure of mitochondrial depolarization; a higher ratio indicated more depolarization. QNT4 treatment of SMMC-7721 cells for 24 h resulted in green JC-1 fluorescence in a dose-dependent manner, which is consistent with a loss of Δψm (Figure 7). After treatment with QNT4 at 2.146 μmol/L, 2.964 μmol/L, and 4.600 μmol/L for 24 h, cellular Δψm decreased to (8.74±3.62)%, (39.64±4.52)%, and (46.90±3.29)%, respectively, compared to the control (P<0.05, T=3.12).
The effect of QNT4 on apoptotic protein expression in SMMC-7721 cells
To characterize the signaling pathways involved in QNT4-induced apoptosis, the expression levels of p53, Bcl-2, Bax, caspase-9, caspase-8, and caspase-3 in QNT4-treated SMMC-7721 cells were analyzed by Western blotting (Figure 8). Mitochondrial release of cytochrome c is a critical step in the apoptotic cascade that can activate downstream caspases. To examine whether QNT4-induced apoptosis in SMMC-7721 cells was associated with the release of cytochrome c from mitochondria, the levels of cytochrome c in both the cytosolic and mitochondrial fractions were analyzed. The results showed that there was a significant increase of cytochrome c in the cytosol and a decrease in the mitochondrial fraction after 24 h treatment with QNT4 (Figure 9).
As a tumor suppressor protein, p53 can induce cell apoptosis in response to stress signals, such as DNA damage. The expression of p53 protein was induced by various concentrations of QNT4 treatment. The anti-apoptotic protein Bcl-2 was also decreased, while the pro-apoptotic protein Bax was increased dramatically by QNT4 treatment. Because changes in Bax/Bcl-2 levels during the initiation of caspase signaling have been reported, caspase-9 and caspase-3 activation was examined in this study as well. Upon apoptotic stimulation, full-length caspase-9 and caspase-3 were cleaved into active fragments. QNT4 treatment (2.964 μmol/L and 4.600 μmol/L) significantly increased the cleaved, activated forms of caspase-9 and caspase-3. These results indicate that QNT4 induced apoptosis in SMMC-7721 cells through the intrinsic mitochondrial apoptotic pathway. On the other hand, QNT4 did not affect the expression of caspase-8, regardless of the treatment dose. Caspase-8 is a prominent initiator of death receptors and is activated by death receptor apoptosis stimuli. Therefore, it seems that QNT4 enhanced apoptosis through the intrinsic mitochondrial apoptosis pathway, whereas the death-receptor signaling pathway played a less important role.
Discussion
Fluoroquinolone compounds have been reported to have an inhibitory effect on cell proliferation and can induce apoptosis in carcinoma cell lines15, 16. In this study, MTT assays indicated that QNT4 inhibited the proliferation of human hepatocarcinoma SMMC-7721 cells in a dose- and time-dependent manner. Thus, we propose that QNT4 could be an effective candidate for therapy against malignant tumors.
Antibacterial fluoroquinolones are a class of antibacterial agents that are commonly used to treat human and animal infections. The treatment of bacterial infection inhibits bacterial DNA gyrase by a mechanism similar to that of certain antitumor drugs against mammalian topoisomerase II17. Some antibacterial fluoroquinolones, such as ciprofloxacin, ofloxacin and norfloxacin, also demonstrate a slight interaction with mammalian topoisomerase II, although these antibacterials are much more selective for bacterial DNA gyrase18. However, a number of chemically modified antibacterial fluoroquinolone derivatives with enhanced activity against mammalian topoisomerase II have been developed. Strong inhibitory effects against eukaryotic DNA replication were demonstrated, and their structure-activity relationship have also been characterized19. These fluoroquinolone derivatives share a similar mechanism of action with several clinically relevant antitumor agents, such as ellipticine and etoposide. They bind to the topoisomerase II–DNA cleavage complexes, thus converting topoisomerase II into a physiological toxin that creates protein-linked DNA breaks in the genome of treated cells20. As shown in Figure 6, QNT4 increased topoisomerase II-mediated supercoiled pBR322 DNA breaks but inhibited topoisomerase II-mediated DNA religation. The effects of QNT4 on the topoisomerase II-mediated DNA cleavage/religation were similar to that of etoposide. These findings provide evidence that QNT4 is a poisonous inhibitor for topoisomerase II21. QNT4 binds the reversible complex between DNA and topoisomerase II, preventing the dissociation of the DNA–topoisomerase II complex and thereby inducing DNA damage22, 23. It has been reported that one of the key responses of fluoroquinolone-induced DNA damage is the expression of tumor suppressor protein p53, causing apoptosis of the treated cells24, 25. The results of this study showed that QNT4 induced cell apoptosis, which was accompanied by the up-regulation of p53 protein level in a dose-dependent manner, suggesting that the activation of the p53 pathway may be involved in the apoptotic process of SMMC-7721 cells.
Apoptosis may be initiated by the stimulation of death receptors located on the cell surface or through an intrinsic pathway involving the release of apoptotic signals from mitochondria26, 27. The cascading activation of caspases and the release of cytochrome c from the mitochondria play key roles in apoptosis, and the type of intracellular apoptotic pathways involved may be deduced from the activated initiator caspases. We specifically investigated the mitochondria-related events during apoptosis, such as the breakdown of the mitochondrial membrane, the expression of Bax and Bcl-2, and the activation of caspase 9. Members of the Bcl-2 protein family play an important role in apoptosis by regulating the release of cytochrome c from the mitochondria to the cytosol28. It has been shown that anti-apoptotic proteins, such as Bcl-2 and Bcl-XL, inhibit cytochrome c release whereas pro-apoptotic members, such as Bax, promote cytochrome c release, leading to the initiation of apoptosis. Here, we observe that QNT4 mediated an up-regulation of Bax and down-regulation of Bcl-2 to induce apoptosis, possibly through increased caspase activity and by preventing the formation of anti-apoptotic bodies. Therefore, it is possible that QNT4 induced the opening of the mitochondrial permeability transition pore through the up-regulation of Bax, resulting in the release of cytochrome c29, 30. In fact, we observed a QNT4-induced decrease of the mitochondrial membrane potential in SMMC-7721 cells, followed by increased cytochrome c release from the mitochondria into the cytosol. In the mitochondrial apoptotic pathway, the release of cytochrome c is a critical event because cytochrome c forms a complex with procaspase-9 in the cytoplasm (resulting in the activation of procaspase-9), which will eventually lead to the activation of caspases 3 and the induction of apoptosis31. Because activated caspase 8, activated by the death receptor, is able to cleave the proapoptotic Bcl-2 family member and then trigger a distinct apoptotic pathway involving mitochondria in some fluoroquinolone compound-treated cell types, it is possible that the activation of caspase 9 in QNT4-treated SMMC-7721 cells may be due to the activation of the death receptor-caspase 8 pathway32, 33. We showed that the cleavage of caspase 8 was not evident in QNT4-treated cells. Therefore, it is unlikely that the activation of caspase 9 is triggered mainly by the caspase 8 pathway, although this possibility cannot be excluded in other cell types34. Thus, we conclude that QNT4 induces apoptosis predominantly through the mitochondrial pathway.
In summary, these data have established QNT4 as a fluoroquinolone derivative that exerts potent anticancer activity through the mechanism of eukaryotic topoisomerase II poisoning. QNT4 also inhibited the proliferation of SMMC-7721 cells through DNA damage and up-regulation of the p53 protein. In addition, QNT4 caused mitochondrial dysfunction, accompanied by an increase in Bax protein expression, a loss of the mitochondrial membrane potential, and the cytoplasmic release of cytochrome c. The subsequent activation of caspase cascades plays a critical role in QNT4-induced apoptosis in human hepatocarcinoma SMMC-7721 cells. Most fluoroquinolone agents can reach concentrations that are far above serum levels in solid tissues, such as the liver33. QNT4 may, therefore, have a potential use as a novel chemotherapeutic agent in the treatment of liver cancer, as well as other solid cancers. The use of QNT4 as a modulating agent may also be interesting in combination with anticancer drugs for future preclinical and clinical studies.
Author contribution
Guo-qiang HU and Bin LIU contributed to the research design; Zhen-yu SHI, Yong-qiang LI, and YU-hua KANG performed the research; Jin-bo DENG and Chao-shen HUANGFU contributed new reagents and analytical tools; Yong-qiang LI analyzed the data; Yong-qiang LI and Bin LIU wrote the manuscript.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (20872028 and 31070952). We are grateful to Prof Jie-xin DENG (Henan University, China) for his critical comments and English revision of the manuscript.
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Shi, Zy., Li, Yq., Kang, Yh. et al. Piperonal ciprofloxacin hydrazone induces growth arrest and apoptosis of human hepatocarcinoma SMMC-7721 cells. Acta Pharmacol Sin 33, 271–278 (2012). https://doi.org/10.1038/aps.2011.158
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DOI: https://doi.org/10.1038/aps.2011.158
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