Abstract
DJ-1 is a multifunctional protein that performs functions in transcriptional regulation and oxidative stress, and the loss of its function is believed to result in the onset of Parkinson's disease (PD). In this study, we report that DJ-1 protects against UV-induced cell death through the suppression of the JNK1 signaling pathway. The results of both binding and kinase studies have revealed that MEKK1 is the direct target of DJ-1. The C-terminus of DJ-1 was crucial to the inhibition of the MEKK1 kinase activity. Wild-type DJ-1 sequesters MEKK1 within the cytoplasm and the L166P mutant facilitates the translocation of MEKK1 toward the nucleus without physical association. Both DJ-1 knockdown and pathogenic L166P mutant were determined to be highly susceptible to the UV-induced activation of the MEKK1-SEK1-JNK1 signaling cascade and cell death. Taken together, our findings show that missense mutation in DJ-1 sensitizes cells to stress-induced cell death through the MEKK1-SEK1-JNK1 signaling pathway, a process, which may trigger the early onset of PD.
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Main
DJ-1 (SP22/CAP-1/RS/PARK7) has been identified as a novel oncogenic protein in transformed NIH3T3 cells, operating in cooperation with H-ras.1 Three other groups of cloned rat homolog of DJ-1/SP22/CAP-1, as a major protein appearing at reduced levels in sperm following the exposure of rats to infertility-inducing toxicants, and also as a regulatory subunit (RS) of an RNA-binding multi-protein complex, which stabilizes mRNA in response to cyclic AMP.2, 3, 4 DJ-1 has recently been shown to be definitively causal in cases of familial Parkinson's disease (PD). Mutations in the DJ-1 gene have been associated with autosomal recessive early onset PD; a homozygous genomic deletion encompassing exon 1–5, a homozygous L166P missense mutation and other homozygous and heterozygous mutations of DJ-1 have been identified in patients with familial or sporadic PD.5, 6, 7, 8, 9 The crystal structure and yeast two-hybrid data indicate that DJ-1 exists as homodimer harboring domains found in both heat shock protein chaperones and ThiJ/PfpI proteases.10, 11, 12 By way of contrast, the L166P mutant exists as a monomer, because it destabilizes the dimer interface. Several lines of evidence suggest that DJ-1 performs a function in the oxidative-stress response13, 14, 15 and also that its expression is induced by oxidative stresses.16, 17, 18 The forced knockdown of DJ-1 expression with short interfering RNA (siRNA) results in susceptibility to oxidative stress, endoplasmic reticulum (ER) stress, and proteasome inhibition.19, 20 The L166P mutant, DJ-1, causes cells to become sensitized to oxidative stress, despite the fact that the pathogenic L166P mutant DJ-1 is highly unstable, and is degraded by the 20S/26S proteasome system.19, 21, 22, 23, 24, 25, 26 Thus far, several lines of evidence also appear to indicate that DJ-1 performs a function in cell death. Firstly, the overexpression of DJ-1 results in PKB/AKT hyperphosphorylation, and enhances cell survival through the suppression of PTEN.27, 28 Secondly, DJ-1 sequesters Daxx within the nucleus, and prevents it from gaining access to the cytoplasm, which keeps Daxx from binding to and activating its effector kinase, apoptosis signal-regulating kinase 1 (ASK1).29 Thirdly, in cultured mammalian cells, DJ-1 has been shown to quench reactive oxygen species and, therefore, protects against reactive oxygen species-induced cell death.19, 29 Nevertheless, despite the many studies conducted regarding the subject, the physiological role of DJ-1 in cell death remains largely unknown. Here, we have demonstrated that DJ-1 modulates the JNK signaling pathway through negative regulation of the functions of mitogen-activated protein kinase/extracellular signal-regulated kinase kinase kinase 1 (MEKK1), through physical interaction.
Results
DJ-1 suppresses oxidative stress-induced JNK1 activation
To determine the possible role of DJ-1 in the regulation of the c-Jun N-terminal Kinase (JNK1) signaling pathway, we subjected human embryonic kidney 293 (HEK293) cells to transient transfection with an expression plasmid encoding for Flag epitope-tagged DJ-1 (Flag-DJ-1), and then evaluated the UV-induced activation of JNK1 in the transfected cells. As UV clearly induced JNK1 activation in control cells, this effect was markedly inhibited in the DJ-1 transfected cells (Figure 1a, Supplementary Figure 1A). We then assessed the UV-induced activation of JNK1 in HEK293 cells that had been stably transfected with an expression vector for Flag epitope-tagged human DJ-1 (HEK293-DJ-1 cells), or with the corresponding empty vector (HEK293-neo cells). The UV-induced activation of endogenous JNK1, which was apparent in the control HEK293-neo cells, was abolished in the HEK293-DJ-1 cells (Figure 1b, Supplementary Figure 1B). To investigate whether the expression and modification of DJ-1 is altered in UV-exposed cells, we performed two dimensional gel electrophoresis experiment. Previous reports have described two main isoforms at pI=5.8 and 6.2.15, 16, 17 Samples were extracted from cells transfected with Flag-tagged wild-type DJ-1, from either untreated cells or cells exposed to UV. We found that the immunoreactivity in the low-pI region of UV-treated cells (Figure 1c). The role of DJ-1 in the JNK1 signaling pathway was further evaluated by the knockdown of DJ-1 and L166P mutant expression using short interference DJ-1 (Supplementary Figures 3A and B). Unlike what was observed in the control short interference plasmid-transfected cells, the cells transfected with short interference DJ-1 harbored low levels of DJ-1 (Figure 1d). The inhibition of endogenous JNK1 activity was restored in accordance with the coexpression of short interference DJ-1 in the HEK293-DJ-1 cells (Figure 1d, Supplementary Figure 1C). Figure 1e also indicates that endogenous JNK1 enzyme activity, which was stimulated by UV, was promoted through the downregulation of DJ-1 expression (Supplementary Figure 1D). These results indicate that the level of DJ-1 expression results in the inhibition of the JNK1 signaling pathway.
DJ-1 inhibits upstream and downstream components of JNK1
We then attempted to determine whether DJ-1 influences signaling upstream of JNK1, including the SEK1 and MEKK1 pathway.30, 31 As UV was found to markedly stimulate the activities of ectopically-expressed SEK1 (data not shown) and MEKK1 (data not shown) in the HEK293 cells, these effects were greatly reduced in DJ-1 transfected HEK293 cells (data not shown). We then determined that UV-induced endogenous SEK1 and MEKK1 activity in HEK293-DJ-1 and HEK293-neo cells. The UV-induced stimulation of endogenous SEK1 (Figure 2a, Supplementary Figure 2A) and MEKK1 (Figure 2b, Supplementary Figure 2B) activities were reduced remarkably in HEK293-DJ-1 cells, in comparison with HEK293-neo control cells. These data showed that DJ-1 overexpression also blocked UV-induced SEK1 and MEKK1 activity.
In the previous reports, it has been suggested that DJ-1 eliminated hydrogen peroxide in vitro by oxidizing itself.19 UV also increases the quantity of intracellular ROS, which is critical with regard to the activation of JNK1 signaling.32 Therefore, we evaluated the role of DJ-1 in the ROS-independent activation of JNK1 using a constitutive active mutant of MEKK1 (ΔMEKK1) (Supplementary Figure 4). Surprisingly, DJ-1 was found to suppress the JNK1 (Figure 2c, Supplementary Figure 2C) and SEK1 (Figure 2d, Supplementary Figure 2D) activity stimulated by ΔMEKK1 in the HEK293 cells. These results indicated that, at least in part, the suppression of the JNK1 signaling pathway by DJ-1 is not connected to ROS scavengers. We then assessed ΔMEKK1 autophosphorylation activities in the HEK293 cells. DJ-1 overexpression prevents the autophosphorylation of ΔMEKK1 (Figure 2e). The inhibition of ΔMEKK1 enzyme activity by DJ-1 was restored according to the coexpression of short interference DJ-1 in HEK293 cells (Figure 2f, Supplementary Figure 2E). Figure 2g also indicates that ΔMEKK1 enzyme activity was promoted through the downregulation of DJ-1 expression. Furthermore, immune complex kinase assay with endogenous MEKK1 revealed that UV treatment increased the kinase activity of endogenous MEKK1 in cells expressing short interference control vector, and that these effects were enhanced in cells expressing short interference DJ-1 (Figure 2h). Thus, our data indicate that MEKK1 may be the principal target protein of DJ-1 in the MEKK1-SEK1-JNK1 signaling cascade through a certain mechanism.
The transcription factor c-Jun is a physiological target of JNK1.33 Thus, we attempted to determine whether DJ-1 influences the JNK1 signaling-dependent increase in the transactivation activity of c-Jun, through a luciferase reporter gene assay. UV caused an increase in c-Jun-dependent luciferase activity and c-Jun Phosphorylation, and this effect was inhibited by DJ-1 (Figure 3a). In HEK293 cells, overexpressed ΔMEKK1 functions as a constitutive active form of the enzyme and enhances c-Jun-dependent luciferase activity. The ΔMEKK1 induced a transcription-stimulating activity of c-Jun, which was blocked by DJ-1 (Figure 3b). Unlike the control short interference plasmid-transfected cells, the short interference DJ-1-transfected cells evidenced an elevated basal level of c-Jun (Figure 3c) and ΔMEKK1-dependent c-Jun transactivation activity (Figure 3c). These results indicate that DJ-1 inhibits the MEKK1-dependent transactivation activity of c-Jun.
DJ-1 inhibits MEKK1 activity, but not SEK1 and JNK1 activities in vitro
The effect of DJ-1 on MEKK1 activity in vitro was assessed to determine whether DJ-1 directly targets MEKK1. Compared to endogenous and ectopic expressed DJ-1, similar level of purified DJ-1 proteins were used in this experiment (Supplementary Figure 3C). An active MEKK1 was acquired through the immunoprecipitation of UV-exposed HEK293 cells using anti-MEKK1 antibody. In the in vitro kinase assay, the pretreatment of active MEKK1 with purified DJ-1 protein resulted in an inhibition of MEKK1 activity (Figure 4a). By way of comparison, DJ-1 pretreatment had little effect on the enzymatic activity of either SEK1 (Figure 4b) or JNK1 (Figure 4c) in vitro. DJ-1 also inhibited the enzymatic activity of ΔMEKK1 (Figure 4d). Furthermore, pretreatment with DJ-1 did not directly influence the activities of any other MAP3Ks in vitro, including MLK3 and TAK1 (Figure 4e). Additional experiment shows that GST and BSA did not suppress the enzyme activity of MEKK1 (Figure 4f). Thus, our data indicate that MEKK1 was the principal target protein of DJ-1 in the MEKK1-SEK1-JNK1 signaling cascade. DJ-1 was not phosphorylated by MEKK1 (data not shown).
DJ-1 interacts physically with MEKK1 in intact cells
We then evaluated the physical interactions occurring between DJ-1 and MEKK1. Coimmunoprecipitation analysis revealed that MEKK1 did associate physically with DJ-1 (Figure 5a). We also attempted to characterize the interaction occurring between MEKK1 and the pathogenic L166P mutant form of DJ-1. We transfected the cells with an increased quantity of the L166P encoding vector to attain an expression level comparable to that of the wild-type DJ-1. Despite the similar amounts of wild-type and DJ-1 (L166P), the interaction between MEKK1 and the mutant was much weaker than with the wild-type DJ-1 (Figure 5a). This result implies that the impaired binding of DJ-1(L166P) to MEKK1 is attributable not to its lower expression level, but rather to a conformational change imposed by the mutation. We also detected a direct interaction occurring between ectopically expressed wild-type DJ-1 and endogenous MEKK1, whereas the L166P mutant of DJ-1 was not detected in an identical immunocomplex with endogenous MEKK1 using HEK293-neo, HEK293-DJ-1, and HEK293-L166P stable cell lines (Figure 5b). We also examined whether endogenous DJ-1 and MEKK1 could interact in intact cells. Immunoblot analysis using anti-DJ-1 antibody of the MEKK1 immunoprecipitates indicated that the physical association of two endogenous DJ-1 and MEKK1 in intact cells (Figure 5c). Immunoblot analysis using anti-DJ-1 antibody of the MEKK1 immunoprecipitates indicated that UV treatment suppresses the physical association of two endogenous DJ-1 and MEKK1 in HEK293 cells. However, exposure of the cells to UV could not prevent the physical interaction between ectopic expressed DJ-1 and endogenous MEKK1 (Figure 5c). Furthermore, we were unable to detect any association between the DJ-1 or L166P mutant and JNK1, or SEK1 by subsequent experiments (data not shown).
Next, we examined the effect of DJ-1 on the physical interaction between two MEKK1 and SEK1 proteins by coimmunoprecipitation. Coimmunoprecipitation assay with endogenous MEKK1 and SEK1 revealed that the binding in cells expressing short interference control vector, and that these effects were enhanced in cells expressing short interference DJ-1 (Figure 5d). Thus, our data indicate that MEKK1 may be the principal target protein of DJ-1 in the MEKK1-SEK1-JNK1 signaling cascade through disruption of binding with downstream substrate.
We then attempted to ascertain which of the domains might be involved in the interaction between MEKK1 and DJ-1. In vitro binding analysis with GST-fused MEKK1 deletion mutants showed that the kinase domain of MEKK1 interacted with DJ-1 (Figure 5e). Subsequent experiments revealed that the carboxyl-terminal (amino acids 165–189) of DJ-1 prevented MEKK1 kinase activity (Figure 5f). The findings of this study indicated that DJ-1 inhibits the activation of MEKK1 without its quenching ability against reactive oxygen species, but that physical interaction is crucial for the function of DJ-1.
DJ-1 sequesters MEKK1 in the cytoplasm and L166P mutant enhances the nuclear accumulation of MEKK1
In an attempt to determine the role of the pathogenic L166P mutant form of DJ-1 in the JNK1 signaling pathway, we conducted an endogenous JNK1 kinase assay using HEK293-neo and L166P mutant stable-expressing cells (HEK293-L166P). The UV-induced activation of endogenous JNK1, SEK1, and MEKK1 apparent in the control HEK293-neo cells was enhanced to a greater extent in the HEK293-L166P cells (Figure 6a). Furthermore, we observed that the DJ-1 mutants (C46A, C53A) induced significant MEKK1 activation, whereas C106A was ineffective in this regard (Figure 6b). MEKK1 is localized principally to the cytoplasm in unstimulated cells. During signal propagation, MEKK1 is translocated to the nucleus and thereby regulate gene expression.34, 35 Wild-type DJ-1 is distributed throughout both the cytoplasm and nucleus. The L166P mutant has been reported to be localized, in part, in both the mitochondria and nucleus.5 We determined that UV stress induces the nuclear accumulation of MEKK1 using confocal study (Figure 6c) and cell fractionation (Supplementary Figure 5, Figures 6e and f). To characterize the effects of the wild-type DJ-1 and L166P mutant on the translocation of MEKK1 from the cytoplasm to the nucleus, the cells were cotransfected with HA-MEKK1, Flag-DJ-1, and Flag-DJ-1 (L166P). The expression of wild-type DJ-1 substantially blocked the UV-induced translocation of MEKK1 into the nucleus (Figures 6c and e). The pathogenic mutant of DJ-1 facilitates the nuclear translocation of MEKK1 (Figures 6c and d). Nuclear localization of MEKK1 in the presence of L166P mutant was corroborated through the immunoblotting of extracts of the nuclear fractions (Figures 6e and f). The results of this experiment demonstrate the protective role of DJ-1 and also show that the stimulatory role of the pathogenic mutant of DJ-1 in the JNK1 signaling pathway might be associated with the regulation of MEKK1 subcellular localization.
Different role of wild-type and L166P mutant DJ-1 against UV-induced cell death
Next, we evaluated the effects of DJ-1 on JNK1-dependent cell death. UV irradiation induced incremental apoptotic cell death in HEK293-neo cells, and this cell death might be repressed by wild-type DJ-1 and nonpathogenic R98Q mutant of DJ-1 (Figure 7a). The results of a recent report have demonstrated the loss of the antiapoptotic activities of DJ-1 mutants.19, 21 In our cell death experiment, we determined that the pathogenic M26I, D149A and L166P mutant of DJ-1 facilitates UV-induced cell death (Figure 7a). We then assessed the effects of wild-type DJ-1 and the L166P mutant of DJ-1 on ΔMEKK1-induced cell death. The overexpression of ΔMEKK1, a constitutively active form of MEKK1, resulted in an increase in apoptotic cell death in HEK293 cells, and this apoptotic effect could be repressed by wild-type DJ-1 (Figure 7b). However, the expression of L166P mutant DJ-1 in HEK293 cells was determined to be more sensitive to ΔMEKK1-induced cell death than that was in wild-type DJ-1 expression, and this function was successfully suppressed by coexpression with the dominant negative form of SEK1 (Figure 7b). These data thus indicate that the pathogenic L166P mutant of DJ-1 was most sensitive to UV-induced apoptotic cell death, and this sensitivity may be dependent on the MEKK1-SEK1-JNK1 pathway. Furthermore, DJ-1 siRNA was found to enhance ΔMEKK1-induced apoptotic cell death (Figure 7c). We then tested the effects of DJ-1 deletion mutants on ΔMEKK1-induced cell death in HEK293 cells transiently transfected with plasmids expressing ΔMEKK1 and the DJ-1 proteins. Our result shows that ΔMEKK1-dependent cell death was repressed by full-length DJ-1 or DJ-1-C, but not by DJ-1-N (Figure 7d). Taken together, these data strongly indicate that DJ-1, through binding to MEKK1, suppresses UV-induced apoptosis, and that the pathogenic L166P mutant of DJ-1 resulted in cell death.
Discussion
We have determined that DJ-1 physically interacts with MEKK1 and inhibits MEKK1 activation, resulting in the suppression of MEKK1-mediated cell death. DJ-1 is a potent inhibitor of the oxidative stress-induced cell death signaling pathway. These observations indicate that the inhibition of MEKK1 is an important mechanism by which DJ-1 operates as a negative regulator of the JNK1 signaling pathway.
DJ-1 prevents the cell death triggered by a variety of apoptotic stimuli, including hydrogen peroxide, dopamine, UV irradiation, and MPP+.19, 20, 21 DJ-1 expression was determined to be induced through oxidative stress.16, 17, 18 Thus far, DJ-1 has been implicated in the modulation of several signaling processes associated with the regulation of cell death. A recent report has demonstrated that the hydrogen peroxide-quenching ability of DJ-1 may not be sufficient to account for its overall cytoprotective functions.29 Our findings in this study represent compelling evidence that wild-type DJ-1 and the pathogenic L166P mutant of DJ-1 evidence identical hydrogen peroxide quenching abilities. However, wild-type and L166P mutant DJ-1 play different roles in the oxidative stress-induced activation of the JNK1 signaling pathway and cell death. Therefore, we can surmise that the hydrogen peroxide quenching ability of DJ-1 is not sufficient to regulate oxidative stress-mediated JNK1 activation and cell death.
The Mouradian group has suggested that DJ-1 may interact with Daxx, sequestering it within the nucleus and preventing it from gaining access to the cytoplasm, thereby blocking Daxx-mediated ASK1 activation.29 Several studies have proposed that dopaminergic neuronal death is increased through the activation of the JNK signaling pathway.36, 37, 38, 39 These observations indicate that JNK may exert an important effect in the mediation of dopaminergic neuronal death, and that the blockage of JNK signaling through specific inhibitors may prevent or effectively retard the progression of PD and other neurodegenerative diseases. Our data now reveal a new mechanism: wild-type DJ-1 directly interacts with and thereby inhibits MEKK1 activation, thus resulting in the suppression of MEKK1-mediated cell death. Considering that MEKK1 is an MAP3K that participates in the JNK signaling cascade, the inhibition of MEKK1 activation by DJ-1 may also be an important mechanism through which DJ-1 inhibits the JNK signaling pathway in the progression of PD.
Deletion and point mutations of DJ-1 have recently been implicated in the onset of familiar PD. In both the previous reports and in this study, the results of cell-death experiments have indicated that DJ-1 gene silencing enhances susceptibility to oxidative stress. These results could explain why the deletion of DJ-1 elicits the early onset of PD. The pathogenic L166P mutant of DJ-1 does not appear to play a pivotal role in ASK1-Daxx-dependent cell death.29 However, the L166P mutant of DJ-1 was identified as a JNK1-dependent cell death inducer, as it was shown to stimulate UV irradiation-induced cell death.21 Our findings also indicate that the L166P mutant of DJ-1 may also participate in the stimulation of the MEKK1-SEK1-JNK1 cascade as well as in UV or ΔMEKK1-induced cell death, thereby suggesting that L166P may play a crucial role in JNK1-dependent cell death. Furthermore, the pathogenic L166P mutant of DJ-1 facilitates oxidative stress-induced cell death, despite its relatively low stability. The findings of this study indicate that DJ-1 may play a critical role in the control of this death pathway, a function which can be abrogated as the result of a PD-causing mutation.
Materials and Methods
Cell culture and transfection
Human embryonic kidney 293 cells were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), penicillin (100 U/ml) and streptomycin (100 μg/ml). For plasmid DNA transfection, the cells were plated at a density of 2 × 106 cells/100-mm dish, grown overnight, then transfected with the appropriate expression vectors in the presence of the indicated combinations of plasmid DNAs, through the calcium phosphate and liposome method. For stable transfection, the HEK293 cells were transfected with pcDNA3 empty vector or pcDNA3-Flag-DJ-1, using Lipofectamine-plus reagent. The total DNA amount was adjusted with carrier vector. After 48 h of transfection, the cells were replated at a dilution of 1/20 and maintained in complete medium containing 500 μg/ml G418 (Invitrogen-GIBCO, USA) to select the neomycin-resistant cells.32
Cloning and preparation of recombinant proteins
A murine MEKK1 gene and deletion mutants were constructed through standard PCR, and inserted into the bacterial expression vector, pGEX4T-1 (Amersham Pharmacia). The MEKK1 deletion mutants constructed in the present study were as follows: MEKK1-N1 (amino-acid residues 1–396), MEKK1-N2 (amino-acid residues 397–821), MEKK1-N3 (amino-acid residues 822–1172), and MEKK1-N4 (amino-acid residues 1173–1493). The expression of the recombinant GST-MEKK1 proteins or pET30a expressing His6-tagged DJ-1 proteins within the transformed bacteria were induced using isopropyl-β-D-thiogalactopyranoside (Sigma). The GST-MEKK1 mutant proteins or His6-DJ-1 proteins were purified with GSH–agarose (Sigma) or Ni2+-NTA–agarose (Qiagen), respectively, in accordance with the manufacturer's instructions. The expression plasmids were verified by automatic DNA sequencing.
Immunocomplex kinase assay
To analyze the kinase activity, confluent cells were harvested and lysed in lysis buffer.40 Cell lysates were then subjected to 10 min of centrifugation at 12 000 × g at 4°C. The soluble fraction was incubated for 1 h with appropriate antibodies against the indicated protein kinases at 4°C. The immunocomplexes were then coupled with protein G-agarose during an additional hour of incubation at 4°C, after which they were pelleted through centrifugation. The immunopellets were rinsed three times with lysis buffer and then twice with 20 mM HEPES, at a pH of 7.4. Immunocomplex kinase assays were conducted through the incubation of the immunopellets for 30 min at 30°C with 2 μg of substrate proteins in 20 μl reaction buffer containing 0.2 mM sodium orthovanadate, 10 mM MgCl2, 2 μCi [γ32P]ATP, 20 mM HEPES, and pH 7.4. The phosphorylated substrates were then visualized through SDS-PAGE, and quantified using a Fuji BAS 2500 phosphoImager.
Two-dimensional gel electrophoresis
For 2DGE, 500 mg of protein was separated first on 13 cm immobilized pH gradient (IPG; Amersham Biosciences) strips using 3.0±10.0 linear gradients according to the manufacturer's instructions on the IPGPhor system (Amersham Biosciences). The second dimension was resolved on 10±20% SDS-PAGE gels and visualized using immunoblot. pI was calibrated using creatine phosphokinase carbamylated standards (Amersham Biosciences) and molecular weight using Precision prestained markers (Fermentas).
Reporter assay
The cells were lysed in chemiluminescent lysis buffer (18.3% of 1 M K2HPO4, 1.7% of 1 M KH2PO4, 1 mM phenylmethyl sulfonyl fluoride (PMSF), and 1 mM dithiothreitol (DTT)) and assayed for luciferase activity with a luciferase assay kit (Promega). The activity of the luciferase reporter protein in the transfected cells was normalized in reference to the β-galactosidase activity in the same cells.32
DJ-1 knockdown in cells
The siRNA targeting DJ-1 was used as described previously.19 Sham control or DJ-1 siRNA was transfected into HEK293 cells using Lipofectamine Plus reagent (Invitrogen), in accordance with the manufacturer's instructions.
Immunofluorescence staining
Assays were conducted as previously described with HEK293 cells plated at 1 × 105 cells per well onto cover slips (Fisher). A total of 0.5 μg of appropriate DNA per well was then transfected using the Geneporter 2 system (Genetherapysystems). The transfected cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS), and then permeabilized with 0.1% Triton X-100 in PBS. Mouse anti-FlagM2 antibody (Sigma) and anti-HA antibody (Novus Biologicals) were used as the primary antibodies at a dilution of 1 : 100. Rhodamine Red or fluorescein-conjugated anti-mouse secondary antibody (1 : 100) was added, and then 4′, 6-diamidino-2-phenylindole (DAPI) staining was applied. The stained cells were evaluated for localization through confocal microscopy (Leica TCS SP5). The final images were obtained and analyzed using confocal microscopy with LAS AF software (Leica).
In vitro binding assay
The recombinant GST fusion proteins were expressed in Escherichia coli strain BL21, using the pGEX system as indicated. The GST fusion proteins were then purified using glutathione-agarose beads (Sigma), in accordance with the manufacturer's instructions. Equal amounts of GST or GST–MEKK1 fusion proteins were incubated with the lysates of HEK293 cells, which had been transfected for 3 h with combinations of expression vectors at 4°C, with rotation. After incubation, the beads were washed three times with ice-cold PBS, and boiled with 20 μl of Laemmli sample buffer. The precipitates were separated through SDS-PAGE, and the pull-down proteins were detected through immunoblotting with specific antibodies.
Coimmunoprecipitation assays
The cells were lysed in 1 ml of RIPA buffer for 30 min at 4°C. After 20 min of centrifugation at 12 000 × g, the supernatants were subjected to immunoprecipitation with appropriate antibodies coupled to protein A-agarose beads. The resulting immunoprecipitates were washed three times with phosphate-buffered solution (PBS, pH 7.4). Laemmli sample buffer was then added to the immunoprecipitated pellets; the pellets were heated for 5 min at 95°C and analyzed through SDS-PAGE. Western blotting was conducted using the indicated antibodies.32
Apoptotic cell death assay using TUNEL staining
The TUNEL staining was carried out using the in situ cell death detection kit (Roche Molecular Biochemicals) according to the manufacturer's protocol as described previously.32 Briefly, cells in culture were fixed and fluorescein-12-dUTP was catalytically incorporated into the fragmented DNA at the 3′-OH by terminal deoxynucleotidyl transferase. The fluorescein-dUTP-labeled DNA was visualized directly by fluorescence microscopy using an excitation wavelength at 488 nm. TUNEL-positive cells were determined from cells for at least 200 nuclei.
Abbreviations
- PD:
-
Parkinson's disease
- siRNA:
-
short interfering RNA
- MEKK1:
-
mitogen-activated protein kinase/extracellular signal-regulated kinase kinase kinase 1
- JNK1:
-
c-Jun N-terminal Kinase
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Acknowledgements
We thank Hiroyoshi Ariga, Mark R Cookson, and Liang Tong for providing the DJ-1 clones and also we thank Drs. Sang Ryeul Lee and Tae-Hoon Lee for technical assistance. This work was supported by a grant from Brain Research Center of the 21st Century Frontier Research Program and Korea Research Foundation (MOEHRD, Basic Research Promotion Fund) (KRF-2005-070-C00105) (H-S, Park) from the Republic of Korea.
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Mo, JS., Kim, MY., Ann, EJ. et al. DJ-1 modulates UV-induced oxidative stress signaling through the suppression of MEKK1 and cell death. Cell Death Differ 15, 1030–1041 (2008). https://doi.org/10.1038/cdd.2008.26
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DOI: https://doi.org/10.1038/cdd.2008.26
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