Abstract
ARTS (Sept4_i2) is a pro-apoptotic tumor suppressor protein that functions as an antagonist of X-linked IAP (XIAP) to promote apoptosis. It is generally thought that mitochondrial outer membrane permeabilization (MOMP) occurs before activation of caspases and is required for it. Here, we show that ARTS initiates caspase activation upstream of MOMP. In living cells, ARTS is localized to the mitochondrial outer membrane. In response to apoptotic signals, ARTS translocates rapidly to the cytosol in a caspase-independent manner, where it binds XIAP and promotes caspase activation. This translocation precedes the release of cytochrome C and SMAC/Diablo, and ARTS function is required for the normal timing of MOMP. We also show that ARTS-induced caspase activation leads to cleavage of the pro-apoptotic Bcl-2 family protein Bid, known to promote MOMP. We propose that translocation of ARTS initiates a first wave of caspase activation that can promote MOMP. This leads to the subsequent release of additional mitochondrial factors, including cytochrome C and SMAC/Diablo, which then amplifies the caspase cascade and causes apoptosis.
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Main
Apoptosis is important for regulating cell numbers and maintaining tissue homeostasis. The main executioners of apoptosis are caspases, a family of cysteine proteases that cleave substrates after aspartate.1 In the mitochondrial pathway, release of pro-apoptotic factors, including cytochrome C (cytoC) and Smac/Diablo (SMAC), from the mitochondrial intermembrane space (IMS) to the cytosol promotes caspase activation. This release requires mitochondrial outer membrane permeabilization (MOMP).2 A holoenzyme complex known as the ‘apoptosome’ is formed when cytoC is released from mitochondria and binds to apoptotic protease-related factor-1 (Apaf-1) to activate procaspase-9.3
The best-studied family of caspase inhibitors is the inhibitor of apoptosis (IAP) proteins.4 IAP proteins contain at least one baculoviral IAP repeat (BIR) domain which can directly interact with caspases and inhibit their apoptotic activity, a RING domain that bestows E3-ubiquitin ligase activity and an Ubiquitin-associated (UBA) domain, which enables the binding of polyubiquitin conjugates via lysine 63.5, 6, 7 X-linked IAP (XIAP) directly inhibits caspases-3, -7 and -9.8 XIAP is considered to be the most potent inhibitor of caspases in vitro, and elevated levels of this protein are found in human cancers.9 Although XIAP-null mice are viable, it was recently shown that loss of XIAP function causes elevated caspase-3 activity and sensitizes certain primary cells toward apoptosis.7 In dying cells, apoptosis can be overcome through the release of caspases from their binding to IAP proteins.10, 11, 12, 13
Several mammalian XIAP antagonists have been identified, including SMAC,14, 15 Omi/HtrA216 and ARTS.17, 18 SMAC and Omi/HtrA2 are located in the mitochondrial IMS, contain a conserved IAP-binding motif (IBM) and are released to the cytosol upon apoptotic induction.14, 15 Genetic inactivation of SMAC and Omi/HtrA2 has failed to reveal any physiological requirement in apoptosis, IAP regulation or tumor suppression,19, 20 possibly because of functional redundancy of different IAP antagonists in the mouse. ARTS (Sept4_i2), derived from the human Sept4 gene, does not contain an IBM, instead it uses unique sequences to bind XIAP.18 ARTS expression is frequently lost in acute lymphoblastic leukemia patients, indicating that it functions as a tumor suppressor protein.21 Sept4/ARTS-null mice show accelerated tumor development, elevated XIAP levels and have stem cells with increased resistance to cell death, demonstrating a physiological role of ARTS for regulating XIAP levels and apoptosis in vivo.22, 23 Furthermore, these phenotypes of Sept4/ARTS-null mice are suppressed by inactivation of XIAP, demonstrating that ARTS acts mainly by targeting XIAP, in vivo.22
It is generally thought that MOMP occurs before activation of caspases and is required for it.2, 24 In this study, we provide evidence that ARTS initiates caspase activation upstream of MOMP. We show that ARTS is localized to the outer membrane of mitochondria (MOM). Following induction of apoptosis, ARTS rapidly translocates to the cytosol in a caspase-independent manner where it binds XIAP. The translocation of ARTS from mitochondria precedes the release of cytoC and SMAC and leads to degradation of XIAP, but not cIAP1, before MOMP. Moreover, knockdown of ARTS inhibits the release of SMAC and cytoC, suggesting that ARTS is required for proper ‘on time’ release of these proteins. This early ARTS-induced caspase activation can promote the cleavage of the BH3-containing protein Bid. We propose that ARTS translocation initiates a first wave of caspase activation through antagonizing XIAP, which leads to de-repression of caspases. According to our model, this initial caspase activity feeds back to mitochondria causing cleavage of Bid and possibly other factors to initiate MOMP, thereby amplifying the caspase cascade leading to apoptosis.
Results
ARTS is localized to the MOM
ARTS localizes to mitochondria of living cells and translocates to the cytosol in response to apoptotic stimuli.17, 18 Other mitochondrial pro-apoptotic proteins, including cytoC and SMAC, reside in the IMS.25, 26 To gain more insight into the mechanism and kinetics by which ARTS is released from mitochondria, we determined the precise localization of ARTS within mitochondria using several distinct approaches. First, we used immunogold labeling with a monoclonal anti-ARTS antibody to visualize ARTS in COS-7 cells. Both endogenous and overexpressed ARTS were detected at the MOM (Figure 1a, white arrows). In order to investigate whether ARTS is readily accessible at the surface of intact mitochondria, mitochondrial fractions of COS-7 cells expressing ARTS were treated with increased concentrations of proteinase K (PK). We observed a reduction in ARTS levels at the highest PK concentration (400 μg/ml) similar to VDAC, a known MOM protein (Figure 1b). In contrast, Hexokinase I, a known peripherally associated protein, was completely digested with all concentrations of PK. Upon treatment with 1% Triton X-100, which solubilizes mitochondria membranes, all shown proteins were digested by even the lowest PK concentration (50 μg/ml) (Figure 1b). Therefore, by this criterion, ARTS appears to be localized to the MOM, similar to VDAC, and the association with MOM protects it from digestion by PK. Next, we treated mitochondrial fractions with alkaline carbonate buffer to determine whether ARTS behaves as a peripheral or integral MOM protein. ARTS, Bcl-XL and Bcl-2 were resistant to carbonate extraction whereas Hexokinase I was carbonate sensitive (Figure 1c). Furthermore, SMAC and cytoC were released into the supernatant fraction during alkaline treatment (Figure 1c), consistent with their known localization in the IMS.25, 26 Finally, treatment of the mitochondrial fraction with increasing concentrations of digitonin demonstrated again that ARTS behaves like an integral MOM protein, very similar to VDAC, Bcl-2 and Bcl-XL (Figure 1d).
Translocation of ARTS from mitochondria precedes the release of SMAC and cytoC
Overexpression of ARTS in certain cultured cells is sufficient to induce the release of cytoC from mitochondria and promote apoptosis.27 ARTS can also induce the release of SMAC to the cytosol (Figure 2a). Several reports have shown that the release of SMAC and cytoC occurs simultaneously.28 Therefore, we considered the possibility that the translocation of ARTS may precede the exit of SMAC and cytoC. In order to compare the kinetics by which endogenous ARTS and SMAC are released from mitochondria, we used HeLa cells that contain relatively high levels of endogenous ARTS and SMAC. Following treatment with staurosporine (STS), we detected cytosolic ARTS as short as 15–30 min after apoptosis induction (Figure 2c; Supplementary Figure S1c). The translocation of ARTS to the cytosol preceded that of SMAC and cytoC by several hours (Figure 2c; Supplementary Figures S1a, c and d). Similar results were seen in SH-SY5Y human neuroblastoma cells, and in mouse embryonic fibroblasts (Supplementary Figures S2a and b), and using UV irradiation in HeLa cells (Figure 6c). Importantly, early translocation of ARTS to the cytosol (30 min) was not blocked by the caspase inhibitor Q-VD, suggesting that it does not require caspase activity (Figure 2d). In contrast, at the later time point (180 min), presumably after MOMP, translocation of ARTS and SMAC was caspase dependent (Figure 2d). Next, to determine whether the early translocation of ARTS to the cytosol affects the release of SMAC and cytoC, we used HeLa cells in which ARTS expression was knocked down by short-hairpin RNAs (shRNAs) (ARTS KD HeLa) (Figure 2b; Supplementary Figure S1f). ARTS KD HeLa cells exhibited a clear reduction in the levels of three different apoptotic markers in response to STS; H2AX, cleaved PARP and active caspase-9 (Figure 2b). This suggests that lack of ARTS protects HeLa cells against apoptosis. Moreover, SMAC and cytoC were found in the cytosol of ARTS KD HeLa cells only after 6 h of STS induction (Figure 2c; Supplementary Figure S1a). Similar results were seen using immunofluorescence (IF) assay with mCherry-tagged ARTS and mCherry-tagged SMAC (Figures 3a and b), demonstrating that loss of ARTS inhibits the release of SMAC to the cytosol. Next, we performed an IF assay with HeLa cells double stained for ARTS and SMAC (Figures 3c and d). Background levels of apoptosis were detected for ARTS and SMAC at time 0 as a result of transfection. However, >70% of cells showed cytosolic ARTS staining after 15 min and >90% after 30 and 60 min of STS treatment. In contrast, a significant release of SMAC into the cytosol was only detected after 180 min of STS induction in 76% of cells, and a significant loss of mitochondrial SMAC staining was also observed at this time (Figure 3d). These results confirm our western blot data that translocation of ARTS to the cytosol precedes that of SMAC. Likewise, HeLa cells stably transfected with cytoC-GFP lost the typical mitochondrial staining for cytoC-GFP and endogenous SMAC after 3 h of STS treatment (Figure 4a). In contrast, endogenous ARTS was detectable in the cytosol already at 15–30 min following STS induction (Figure 4b). To confirm that the early increase in levels of ARTS at the cytosol reflects the translocation of mitochondrial ARTS rather than newly synthesized ARTS which is not properly inserted into MOM, we performed a fractionation assay on HeLa cells in the presence or absence of inhibitor of protein translation cycloheximide (CHX). Supplementary Figure S1eII demonstrates accumulation of ARTS in the cytosol of CHX-treated cells indicating, that at least some, if not the majority of ARTS present in the cytosol following apoptotic induction, is caused by its rapid translocation from mitochondria. Together, these results reveal that ARTS translocates to the cytosol before cytoC and SMAC, and that the function of ARTS is required for the proper ‘on time’ release of cytoC and SMAC from mitochondria during apoptosis.
An ARTS-XIAP complex forms rapidly in response to apoptotic stimuli, and XIAP levels decrease before the release of cytoC and SMAC
ARTS can induce apoptosis, at least in part, by binding to XIAP,17 but the kinetics by which an ARTS-XIAP complex forms have not been determined. In order to visualize the interaction of ARTS and XIAP, we used the split-Venus, Bimolecular Fluorescence Complementation (BiFC) system.29 The fluorescent signal that serves as a measurement for ARTS-XIAP proximity was evaluated by IF (Figure 5a) and flow cytometric analyses (Figure 5b). Both approaches show that fluorescence indicative of ARTS-XIAP complex formation can be seen as early as 15 min after STS treatment. The fluorescent signal continues to increase and peaks at 60 min after STS induction. Since we never observed the release of either cytoC or SMAC at time points earlier than 3 h after STS induction, we conclude that ARTS forms a complex with XIAP before MOMP.
We also investigated the levels of XIAP, cIAP1 and other apoptotic proteins during STS treatment (Figure 6a), and in response to UV irradiation (Figure 6c). This revealed that XIAP, but not cIAP1 levels were decreased 30–120 min after STS induction. Over this time period, we also observed processing of caspase-9 (Figure 6a). Significantly, knockdown of ARTS blocked the decrease of XIAP levels and also delayed caspase processing (Figures 6a and b). Although the signal representing the ARTS-XIAP complex increases for the first hour after induction of apoptosis (Figures 5a and b), XIAP protein levels decline (Figure 6a). This indicates that the fraction of XIAP that is bound to ARTS increases during the first hour of apoptosis induction while there is concomitant degradation of total XIAP protein. By 180 min after STS induction, the ARTS-XIAP-Venus signal is significantly decreased, presumably due to the overall reduced XIAP levels. This suggests that a complex between ARTS and XIAP forms rapidly upon induction of apoptosis and before MOMP, and that the early function of ARTS promotes caspase activation.
The early translocation of ARTS to the cytosol promotes activation of caspase-9 and cleavage of Bid
Cleavage of the pro-apoptotic Bcl-2 family member Bid by caspases has been shown to promote MOMP and the release of cytoC.30, 31 To determine whether ARTS influences MOMP, and the release of cytoC and SMAC via Bid, we examined the levels of truncated Bid (tBid) in HeLa and ARTS KD HeLa cells following STS treatment. Bid levels are reduced 30 min after UV induction (Figure 6c) and tBid appears 60 min after STS induction (Figure 6a). Since the reduction of Bid levels is blocked by caspase inhibitors we attribute it to caspase cleavage (Figure 6d). Furthermore, only small amounts of tBid were seen 180 min after STS induction in ARTS KD HeLa cells, indicating that ARTS is required for promoting the observed activation of Bid (Figure 6a). Importantly, the presence of cleaved caspase-9 was demonstrated in HeLa cells, 30 and 60 min after STS and UV treatment (Figures 6a–c, respectively) and caspase-9 activity was demonstrated 60 min following STS induction (Figure 6b). Interestingly, in ARTS KD HeLa cells activation of caspase-9 was significantly delayed (Figure 6a), and activation of caspase-3 could not be detected (Figures 6a and c). Caspase-2 and caspase-3 activation was detected only after 3 h in HeLa cells (Figures 6a and c). We could not detect activation of caspase-8 following STS treatment (data not shown). Caspase-9 was shown to promote truncation of Bid.32 Thus, it seems that ARTS can promote MOMP, at least in part, through inducing caspase-9 activation and cleavage of Bid. An early decrease in XIAP and Bid, but not in cIAP1 levels was seen 30 min after STS as well as UV induction (Figures 6a and c). This early reduction of XIAP is associated with caspase-9 activation and Bid cleavage (Figures 6a–c) and ARTS seems to be required for it (Figures 6a and b). Downregulation of cIAP1 was seen only after 180 and 360 min of STS treatment (Figures 6a and 8c1). We, therefore, suggest that de-repression of caspase-9 occurring 30–60 min following apoptotic induction by binding of ARTS to XIAP, can lead to cleavage of Bid which is known to feedback to mitochondria and induce MOMP and the release of cytoC.
Inactivation of ARTS in HeLa cells leads to resistance toward apoptosis
To assess cell death in HeLa versus ARTS KD HeLa cells, we have performed several assays including XTT assay, counting of DAPI-stained nuclei, clonogenic survival assay and counting of terminal deoxynucleotidyltransferase-mediated deoxyuridine triphosphate nick end-labeling (TUNEL)-positive cells (Figure 7). All four different assays showed reduced cell death in ARTS KD cells following treatment with STS when compared with wt HeLa (Figures 7a–d). These data support our hypothesis that early activation of caspases induced by ARTS promotes cell death.
ARTS promotes rapid and specific degradation of XIAP but not cIAP1 protein upon induction of apoptosis
Upon induction of apoptosis, XIAP protein is degraded by the ubiquitin-proteasome system (UPS).4 SMAC and/or small-molecule derivatives (‘SMAC-mimetics’) selectively reduce the levels of cIAP1 and cIAP2, but not that of XIAP.33 ARTS can bind to multiple IAP family members; cIAP1 (Figure 8a), ML-IAP (Livin) (data not shown) and XIAP (Figures 5a, b and 8a; Gottfried et al.17). Therefore, we investigated the possibility that ARTS promotes degradation of IAPs. First, we overexpressed ARTS and found this to strongly reduce endogenous XIAP protein levels, but not cIAP1 (Figure 8b). Next, we treated HeLa cells with STS and evaluated XIAP and cIAP1 levels at different incubation times (Figure 8cI). We saw that XIAP steady-state protein levels began to decrease already 30 min after STS induction, and this decrease continued for several hours. In contrast, there was no detectable change in cIAP1 protein levels over the 3-h induction (Figure 8cI). Moreover, incubation of the cells with the proteasome inhibitor MG132 blocked most of the decline of XIAP protein. This indicates that XIAP is degraded by the UPS in cells stimulated to undergo apoptosis, consistent with earlier reports (Figure 8cI; Salvesen and Duckett4). In order to investigate the function of endogenous ARTS for controlling XIAP levels, we compared XIAP and cIAP1 levels in HeLa versus ARTS KD HeLa cells treated with STS (Figure 8cII). We found that knockdown of ARTS blocked the decrease of XIAP protein almost as well as MG132 (Figure 8cII). Taken together, our results suggest that ARTS is required for the rapid, early reduction of XIAP in response to STS treatment.
Discussion
The release of pro-apoptotic mitochondrial factors, such as cytoC and SMAC, has been traditionally viewed as the initiation stage of the mitochondrial pathway, promoting caspase activation. This redistribution of cytoC and SMAC from mitochondria to the cytosol requires MOMP.24 Although several studies indicate that the release of SMAC and cytoC can occur independently of caspases,34 others suggest that caspase activity is required for this.35, 36 Here, we provide evidence that the mitochondrial IAP-antagonist ARTS may be the ‘missing link’ enabling MOMP, and the translocation of cytoC and SMAC in paradigms where this release depends on caspase activation. In particular, we suggest that ARTS has a critical role in initiating the mitochondrial apoptotic pathway upstream of MOMP, and that it acts by a different mechanism than other known IAP antagonists.
The first phase of ARTS translocation from mitochondria as well as binding of ARTS to XIAP occurs in a caspase-independent manner (Figure 2d; Gottfried et al.17). Unlike SMAC, which resides in the IMS,25 ARTS is localized to the MOM (Figures 1a–d). Since ARTS does not contain any known trans-membrane domain, ARTS is either partially or fully embedded in the MOM, or it is covalently linked to integral MOM proteins. The localization of ARTS is highly relevant for understanding the kinetics by which ARTS translocates from mitochondria compared with SMAC and cytoC.
Despite a large number of studies, the precise timing of the release of SMAC and cytoC from mitochondria remains controversial. Several reports concluded that SMAC exits the mitochondria simultaneously with cytoC,28 while others have argued that the release of cytoC precedes that of SMAC.37 We now show that ARTS translocation precedes the release of both cytoC and SMAC (Figures 2c, 3a, c and 4b; Supplementary Figures S1a, c and d), and that downregulation of ARTS significantly delays the release of both proteins (Figures 2c and 3b; Supplementary Figure S1a). Therefore, ARTS is required for the normal ‘on time’ release of cytoC and SMAC and functions upstream of MOMP.
Consistent with the idea of a biphasic action of ARTS in antagonizing XIAP, we found a progressive release of ARTS from the MOM (Supplementary Figure S1b). Both XIAP and cIAP1 levels are downregulated in cells undergoing apoptosis.4 We confirmed these results and also found a coordinated decrease in both XIAP and cIAP1 levels 3–6 h after apoptosis induction (Figures 6a, c and 8c). However, we observed a selective reduction of XIAP, but not cIAP1, at a much earlier stage, 30 min of STS and UV induction (Figures 6a, c and 8c). This early reduction of XIAP protein was blocked by knockdown of ARTS in HeLa cells (Figures 6a and 8cII) and in Sept4/ARTS-null mice,22 suggesting that the early reduction of XIAP can be mainly attributed to ARTS. We also observed a consistent increase in XIAP levels in different tissues from Sept4/ARTS-null mice (data not shown). In contrast, cIAP1 levels were not altered in these mice. This demonstrates that ARTS has a physiological and selective role for determining XIAP protein levels in vivo. Treatment of cells with the proteasome inhibitor MG132 blocked ARTS-induced reduction of XIAP levels, indicating that ARTS promotes degradation of XIAP via the UPS (Figure 8c). XIAP is an ubiquitin-ligase and its E3-ligase activity is important for both self-conjugation and caspase regulation.4, 7 Therefore, ARTS may stimulate auto-ubiquitination of XIAP, or promote degradation of XIAP by activating another E3 ligase involved in this process, such as Siah1.38 We also observed cleavage of XIAP hours before MOMP in HeLa, but not in ARTS KD HeLa, suggesting that ARTS is required for caspase-dependent cleavage of XIAP (data not shown).
Pro-apoptotic Bcl-2 family members, such as Bax, Bak and Bid, promote MOMP and the release of pro-apoptotic mitochondrial proteins.39 Bid can be cleaved by caspases-3, -9 and -8, and cleaved Bid promotes MOMP.31, 32 Therefore, tBid is one attractive candidate by which ARTS-induced caspase activation may feedback to mitochondria and promote MOMP. Consistent with a possible role of Bid, caspase-mediated cleavage of Bid was observed 60 min following STS induction (Figure 6a). This effect was significantly delayed in ARTS KD HeLa cells, suggesting that ARTS is required for the early cleavage of Bid. However, it is unlikely that Bid is the only target for ARTS-induced MOMP.
Several recent publications suggest that initiation of apoptosis in mammalian cells can occur through de-repression of caspases from their binding to IAP proteins.12, 13 Our results provide a novel perspective to explain this phenomenon. The location of ARTS at the MOM may explain why ARTS translocates to the cytosol in a caspase-independent manner, before other mitochondrial pro-apoptotic proteins, which reside in the IMS.
In summary, we show that ARTS inhibits XIAP by a mechanism that is distinct from any other known mammalian IAP antagonist,40 and that it has an important role in initiating the mitochondrial pathway. We suggest that an initial caspase-independent exit of ARTS from mitochondria acts rapidly to specifically reduce XIAP protein levels. According to our model (Figure 9), this causes direct activation of low, non-lethal levels of caspase activity (Figure 6) and reduces the threshold toward apoptosis. Upon sustained apoptotic stimuli, elevated caspase levels are expected to feedback to mitochondria, at least in part by cleaving Bid, and promote MOMP, thereby initiating the non-reversible amplified phase of apoptosis (Figure 9). The exact mechanism by which caspase activation promotes MOMP remains to be elucidated, but this is beyond the scope of this study. Because ARTS acts very early in the initiation of a caspase cascade and is lost in human cancers, ARTS-based drugs may offer new opportunities to manipulate apoptosis for therapeutic purposes.
Materials and Methods
Antibodies
Antibodies to the various proteins were purchased from the indicated companies, and used as instructed. Importantly, unless noted otherwise, in all our assays we used the monoclonal anti-ARTS antibody (Sigma-Aldrich, Saint Louis, MO, USA), which is the only currently commercially available antibody directed against the unique C-terminus of ARTS. Antibodies against Hexokinase I (G-1, sc-46695), VDAC (N-18, sc-8828), Bcl-XL (A-20, sc-7122), Bcl-2 (N-19, sc-492), GAPDH (FL-335, sc-25778) and GST (B-14, sc-138) were purchased from Santa Cruz (Santa Cruz, CA, USA); antibodies against SMAC (#567365) and H2AX (DR1016) are from Calbiochem (La Jolla, CA, USA). Additionally, antibodies against cytoC (2050_9, Clontech, Mountain View, CA, USA), XIAP (#610716, BD, San Jose, CA, USA), cIAP (AF818, R&D Systems, Minneapolis, MN, USA), Bid (ab2561, Abcam, Cambridge, UK; #2002, Cell Signaling), cleaved Caspase-9 (D315, #9505, Cell Signaling, Beverly, MA, USA), cleaved Caspase-3 (D175, #9661, Cell Signaling), Caspase-2 (ADI-905-685, Enzo Life Sciences, Plymouth Meeting, PA, USA), PARP (2660, SA-253, Biomol, Tebu, Le Perray en Yvelines, France) and actin (#69100, MP Biomedicals, Solon, OH, USA) were used.
Mammalian cell cultures and various treatments
COS-7, HeLa cells were grown in Dulbecco's modified Eagle medium (DMEM) with 4.5 g/l D-glucose. All media were supplemented with 10% fetal calf serum (FCS), penicillin 100 U/ml, streptomycin 100 mg/ml, sodium pyruvate 1 mM and L-glutamine 2 mM (Biological Industries, Kibbutz Beit Haemek, Israel). HeLa cells stably transfected with cytoC-GFP were kindly provided by Douglas Green and Sandy Simon. To induce apoptosis, the cells were incubated with STS (Sigma), or exposed to UV irradiation for 5 min. MG132 (20 μM, Sigma) for 6 h was used to inhibit proteasome activity in cells. To inhibit caspase activity, the cells were incubated with the pan-caspase inhibitor Q-VD (3 μM) for 2 h before treatment with pro-apoptotic factors.
Constructs
pEF1-AU5 and pEF1-AU5-ARTS constructs contain an AU5 tag that is attached to the N-terminus of ARTS.20 ARTSD4aa – mutant ARTS lacking the first 4 aa was generated in our laboratory. The pSC2-6Myc-ARTS was generated using PCR with the following primers: F BamHI-5′-TCGAGGATCCATCAAGCGTTTCCTGGAGGACACCACGG-3′ and the reverse primer: pEG ARTS-R TACCGCTCGAGCTAGTGGCAGCCCTGCCC. The pEBG mammalian expression construct encoding N-terminus GST fusion protein with or without XIAP was a kind gift from Colin Duckett. mCherry-ARTS borrowing the mCherry tag within the ARTS was produced by GenScript (Piscataway, NJ, USA), while mCherry-SMAC was a kindly gift from Sandy Simon.
Transient transfection of cells
jetPEI (Polyplus Transfection Inc., New York, NY, USA) and Transfectol (GeneChoice, Frederick, MD, USA) were used according to the manufacturer’s protocol.
Western blot analysis
Western blot analysis was performed as described in Lotan et al.29 Visualization was performed using LAS4000 luminescent image analyzer (Fujifilm, Tokyo, Japan). Densitometry analyses of western blot results were done using TotalLab TL100 graphic software (Nonlinear Dynamics Ltd, Durham, NC, USA).
Transmission electron microscopy imaging of ARTS immunogold labeling
The transmission electron microscopy (TEM) imaging of ARTS immunogold labeling was carried out at the Electron Microscopy Unit, Weizmann Institute of Science, Rehovot, Israel. COS-7 cells were prepared for immunogold labeling according to the Tokuyasu method. For single immunogold labeling, the sections were incubated with anti-ARTS antibody (1 : 50 dilution), followed by anti-mouse coupled with 9 nm gold antibody, all in blocking medium (5% FCS, 0.15% glycine in PBS). The sections were washed with PBS and H2O, incubated with 0.3% uranyl acetate, 1.8% methylcellulose on ice and air dried. Staining using only secondary antibody was used as negative control. The immunogold-labeled sections were examined in an electron microscope (Tecnai-12, FEI, Eindhoven, Netherlands, operating at 120 kV).
Mitochondria isolation for localization assays
Mitochondria-enriched fraction was obtained by cell homogenization with Dounce homogenizer in 20 mM HEPES-KOH pH 7.5, 10 mM KCl, 1.5 mM MgCl2, 1 mM sodium EDTA, 1 mM sodium EGTA and 1 mM DTT in the presence of 250 mM sucrose and protease inhibitors (Mini-Complete, Roche Diagnostocs, Indianapolis, IN, USA). Homogenates were centrifuged at 500 × g for 5 min at 4°C, and the supernatant was centrifuged at 10 000 × g for 20 min to obtain mitochondria. Mitochondria were washed with homogenization buffer.
PK treatment of mitochondrial fractions
Purified mitochondria were resuspended in Tris buffer (30 mM Tris pH 7.6, 1 mM CaCl2). The suspension was divided into equal aliquots for PK treatment. Aliquots of mitochondria were treated with 50 and 400 μg/ml PK in a total volume of 100 μl. PK digestion reactions were incubated in ice for 30 min. PMSF was added to a final concentration of 2 mM to terminate the PK activity, followed by 5 min of incubation. Digested mitochondria were pelleted by centrifugation at 13 000 × g for 20 min, washed in homogenization buffer and resuspended in SDS-PAGE sample buffer. To confirm the activity of the PK, the mitochondrial fraction was solubilized with 1% Triton X-100, incubated with PK, centrifuged at 13 000 × g, and the supernatants were analyzed by western blotting as described previously.
Digitonin permeabilization
For digitonin permeabilization, isolated mitochondria were incubated with 0–6 mg/ml digitonin (final concentration) for 30 min on ice. Following digitonin treatment, the samples were centrifuged at 16 000 × g for 20 min at 4°C. The supernatant was subjected to SDS-PAGE and western blotting.
Carbonate extraction of mitochondria
Mitochondria-enriched fraction, which was prepared as described above, was resuspended in homogenization buffer and 10 μl were further divided into half for carbonate treatment. In all, 5 μl of mitochondria suspension was treated with 20 μl of 0.1 M sodium carbonate pH 11.5, whereas the other 5 μl mitochondrial suspension was mock treated with 20 μl sucrose 0.25 M in 0.1 M Tris-HCl pH 7.5 for 30 min in ice. Supernatants were retained after treatment by centrifugation at 50 000 r.p.m. for 15 min at 4°C and were resuspended in SDS-PAGE sample buffer. The pellets were washed in homogenization buffer and resuspended in SDS-PAGE sample buffer.
Fluorescence assay
For all experiments, cells were seeded in 24-well plates on coverglasses previously coated with fibronectin (5 μg/ml, Biological Industries). Following apoptotic induction (1.75 μM STS), cells were fixed with 4% paraformaldehyde in PBS for 20 min at room temperature and washed with PBS. For fluorescence assay, HeLa cells were transiently transfected with mCherry-ARTS or mCherry-SMAC and stained with DAPI (#157574, MP). Image analysis was carried out using fluorescent microscope (Nikon 50i, Kawasaki, Kanagawa, Japan). For IF assays, HeLa cells were co-transfected with AU5-ARTS and Flag-SMAC and stained with MitoTracker Red (M-7512, Molecular Probes, Eugene, OR, USA). HeLa cells stably transfected with cytoC-GFP were used for visualization of endogenous proteins. In both experiments, IF staining were preformed with anti-ARTS and anti-SMAC antibodies followed by fluorescent secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA, USA). Visualization was carried out using Nikon 50i fluorescent microscope for endogenous proteins, and with Zeiss AxioPhot (Carl Zeiss AG, Feldbach, Switzerland) for the transfected proteins.
GST pull-down binding studies
COS-7 cells were co-transfected with GST empty vector, GST-XIAP or GST-cIAP1 (in pEBG, mammalian GST expression vector) together with pCS2-6Myc-ARTS. The cells were lysed in RIPA buffer (150 mM NaCl, 50 mM Tris-HCl pH 8, 1% NP-40, 0.5% deoxycholate acid containing protease inhibitors; Mini-Complete, Roche). The samples were left rotating for 4 h at 4°C with the GST fusion proteins coupled with the glutathione beads. Samples were centrifuged at 4000 r.p.m. at 4°C for 4 min and washed five times in lysis buffer. Proteins were eluted from beads following 5 min of boiling in sample buffer and separated by SDS-PAGE, followed by western blot analysis.
Cell fractionation by digitonin
Following induction of apoptosis, cytosolic fraction was generated using a digitonin-based subcellular fractionation technique, as described in Adrain et al.38 Special care was taken while performing this procedure to avoid translocation of ARTS from mitochondria to the cytosol at time 0, when cells were not treated with pro-apoptotic inducer, and ARTS was localized to the MOM. Briefly, cells were harvested and centrifuged at 300 × g for 10 min, washed in TBS 2.5 mM pH 7.5 and repelletted. Cells were permeabilized for 5 min on ice with cytosolic extraction buffer (250 mM sucrose, 70 mM KCl, 137 mM NaCl, 4.3 mM Na2HPO4, 1.4 mM KH2PO4 pH 7.2, protease inhibitor cocktail; Complete, Roche) containing freshly prepared digitonin (200 μg/ml, D-5628, Sigma). Cytosolic fraction was isolated by collecting the supernatant after centrifugation at 1000 × g for 5 min at 4°C. Cytosolic fraction was separated by SDS-PAGE and transferred onto nitrocellulose membranes.
Generation of ARTS KD constructs
Four small interference RNA sequences were identified according to the Whitehead siRNA Selection Web Server and Oligoengine shockwave program as follows.
shRNA1: 5′-gatccccGCCTGAGGCTCCTGGCACCttcaagagaGGTGCCAGGAGC CTCAGGCttttta-3′ and complementary strand, 5′-agcttaaaaaGCCTGAGGCTCCTGGCACCtctcttgaaGG TGCCAGGAGCCTCAGGCggg-3′;
shRNA2: 5′-gatccccGGGAACAGGCCAAGACACTtcaagagaG TGCTCTTGGCCTGTTCCCttttta-3′ and complementary strand, 5′-agcttaaaaaGGGAACAGGCCAAGAGCACtctcttgaaGTGCTCTTG GCCTGTTCCCggg-3′;
shRNA3: 5′-gatccccCAGGCCAAGAGCACCAGGGttcaagagaCCCTGGTGCTCTTGGCCTGttttta-3′ and complementary strand, 5′-agcttaaaaaCAGGCCAAGAGCACCAGGGtctcttgaaCCCTGGT GCTCTTGGCCTGggg-3′;
shRNA4: 5′-gatccccGAGCACCAGGGGCAGGGCTttcaagagaAGCCCTGCCC CTGGTGCTCttttta-3′ and complementary strand, 5′-agcttaaaaaGAGCACCAGGGGCAGGGCTtctcttgaaAGCCCTGCCC CTGGTGCTCggg-3′;
shRNA for lacZ (control shRNA): 5′-gatccccCTTAATCGCCTTGCAGCACttcaagagaGTGCTGC AAGGCGATTAAGttttta-3′ and complementary strand, 5′-agcttaaaaaCTTAATCGCCTTGCAGCACtctcttgaaGTGCTGCAAG GCGATTAAGggg-3′.
The 3′-and 5′-ends of the oligonucleotide primers were adapted for cloning into the BglII and HindIII site of the pSUPER vector. Equimolar amounts of oligonucleotide pairs were annealed and ligated into pSUPER digested with BglII and HindIII. Positive clones were sequenced to ensure accuracy of RNA interference. The efficiency of RNA interference was initially tested by transfecting each shRNA construct into HeLa cells and performing western blot analysis. shRNA4 reduced ARTS protein levels when compared with control cells.
Generation of ARTS KD HeLa cells
To generate HeLa cells stably expressing ARTS shRNA, subconfluent cultures were transfected with shRNA4 construct or the control shRNA construct (2 μg per 35 mm plate), together with pCDNA 3.1 for selection (1 : 20 ratio). Transfection was performed with jetPEI reagent (Polyplus transfection) according to the manufacturer’s instructions. Following selection with 0.5 mg/ml G418 (Sigma), mass cultures were tested for the reduction of endogenous ARTS protein. These cells were used in this study.
BiFC assay examining ARTS-XIAP direct binding
The split-Venus, BiFC system31 was used to evaluate the kinetics of ARTS and XIAP complex formation in HeLa cells following STS treatment. For this purpose, ARTS and XIAP were fused to the split-Venus proteins (a brighter and more photostable variant of YFP) – VC 155–239 and VN 1–173, respectively. These constructs were expressed by transient transfection in HeLa cells together with pdsRED plasmid, which was used as a transfection efficiency marker.
Flow cytometry analysis of BiFC-transfected cells
Transfection was performed using 200 ng of each plasmid along with 50 ng of pdsRED. Thirty-six hours later, 1.75 μM STS was added to HeLa cells for the indicated time periods to induce apoptosis. Cells were trypsinized, washed with PBS and resuspended in 0.5 ml PBS. Flow cytometry analyses of cell suspensions were performed using FACS (FACSCantoII; BD Biosciences, San Jose, CA, USA) equipped with an argon laser emitting at 488 nm. Analysis was restricted to live cells. Results were analyzed using FACSDIVA software (BD Biosciences). To normalize the results according to transfection efficiency, ratio between YFP and Red fluorescence was calculated for each time point and represented relatively to that without apoptotic induction (t=0).
IF analysis of BiFC-transfected cells
HeLa cells were seeded on coverslides placed in 24-well plates. Cells were transfected with 100 ng of each plasmid (XIAP-VN and ARTS-VC) along with 25 ng of pdsRED. Thirty-six hours after transfection, the cells were treated with STS for the indicated time periods to induce apoptosis. Then, cells were fixed in 4% PFA and washed with PBS. Image analysis was carried out using confocal laser microscopy (Zeiss LSM 510, Carl Zeiss AG).
Caspase-9 activity assay
Caspase-9 activity was determined in HeLa and ARTS KD HeLa cells using measuring cleavage of fluorescently labeled substrate of caspase-9 (Caspase-9 Fluorometric Assay Kit, K118, BioVision, Mountain View, CA, USA) according to the manufacturer's instructions.
In situ cell death detection assay – TUNEL
HeLa and ARTS KD HeLa cells were treated with STS for different time periods. TUNEL assay (In Situ Cell Death Detection Kit; Roche, #12-156-792-910) was performed on the slides followed by image analysis using fluorescent microscopy (Zeiss AxioPhot). DAPI stain (MP, 157574) was used to assess total cell number. Ratio of TUNEL-positive cells out of total cells represented the number of apoptotic cells. The percentage of TUNEL-positive cells was calculated as the number of TUNEL-positive cells divided by the total number of cells.
XTT viability assay
XTT cell viability assay was performed in 96-well plate according to the manufacturer's instructions (Cell proliferation kit – XTT-based colourometric assay, Biological Industries), following STS (1.75 μM) treatment of cells for the indicated time periods. Absorbance of the water-soluble formazan salt was measured on PowerWaveXS Elisa reader (BioTek, Winooski, VT, USA) at primary wavelength of 450 nm and a reference wavelength of 630 nm. Each plate contained the samples, positive control, negative control and blank. The assay was performed in quadricates and the results were presented as a ratio values between each sample and the negative control ±S.E.
Clonogenic assays
Apoptosis was induced in HeLa and ARTS KD HeLa cells by 1.75 μM STS. Then, cells were split and 300 cells were seeded in 6-cm dishes and allowed to grow for 12 days. Colonies >50 cells were counted.
Survival assay
HeLa and ARTS KD HeLa cells were treated with STS for different time periods. In all, 4% paraformaldehyde was used to fix the cells for 30 min, followed by two repeated washes with PBSx1. Permeabilization was performed using 0.5% Triton X-100 for 5 min. Staining with DAPI (MP, 157574) and scoring cells were done using fluorescent microscope Nikon 50i. Counts for each sample represent the average number of viable cells±S.E. in eight different fields.
Abbreviations
- BIR:
-
baculoviral IAP repeat
- CytoC:
-
cytochrome C
- IAP:
-
inhibitor of apoptosis proteins
- IF:
-
immunofluorescence
- IMS:
-
intermembrane space
- KD:
-
knockdown
- MOM:
-
mitochondrial outer membrane
- MOMP:
-
mitochondrial outer membrane permeabilization
- PK:
-
proteinase K
- shRNA:
-
short-hairpin RNA
- STS:
-
staurosporine
- UPS:
-
ubiquitin-proteasome system
- XIAP:
-
X-linked IAP
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Acknowledgements
We thank John Silke, Colin Duckett, Sandy Simon, Chunying Du, Douglas Green and Changdeng Hu for generously providing us with materials. We are very grateful to Michael Loewenstein, Sagie Schif-Zuck, Alana Bahjan Persaud and Travis Gorenc for technical assistance and to Hermann Steller for critical reading of this manuscript. This work was supported by funds from BSF (US Israel Binational Science Foundation) grant #2003085 (to SL), ISF (Israel Science Foundation) grant #1264/06 (to SL), a grant from Israel Cancer Association (ICA) (to SL), and by a generous donation from the Charles Wolfson Charitable Trust, England.
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Edison, N., Zuri, D., Maniv, I. et al. The IAP-antagonist ARTS initiates caspase activation upstream of cytochrome C and SMAC/Diablo. Cell Death Differ 19, 356–368 (2012). https://doi.org/10.1038/cdd.2011.112
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DOI: https://doi.org/10.1038/cdd.2011.112
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