Abstract
The TP53 tumour-suppressor gene is expressed as several protein isoforms generated by different mechanisms, including use of alternative promoters, splicing sites and translational initiation sites, that are conserved through evolution and within the TP53 homologues, TP63 and TP73. Although first described in the eighties, the importance of p53 isoforms in regulating the suppressive functions of p53 has only become evident in the last 10 years, by analogy with observations that p63 and p73 isoforms appeared indispensable to fully understand the biological functions of TP63 and TP73. This review summarizes recent advances in the field of ‘p53 isoforms’, including new data on p63 and p73 isoforms. Details of the alternative mechanisms that produce p53 isoforms and cis- and trans-regulators identified are provided. The main focus is on their biological functions (apoptosis, cell cycle, aging and so on) in cellular and animal models, including mouse, zebrafish and Drosophila. Finally, the deregulation of p53 isoform expression in human cancers is reviewed. Based on these latest results, several developments are expected in the future: the identification of drugs modulating p53 isoform expression; the generation of animal models and the evaluation of the use of p53 isoform as biomarkers in human cancers.
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Thirty years of research have shown that the p53 tumour-suppressor protein, encoded by TP53 gene (OMIM 191170), integrates endogenous and exogenous signals to modulate cell fate to stress and cellular environments.1 In addition, it has emerged that p53 is more than just a ‘stress response’ factor as it regulates embryo implementation.2 The ability of p53 to integrate signals implies the existence of multiple and subtle levels of regulation. Over the years, transcriptional, translational and post-translational regulatory mechanisms have been uncovered.3, 4 This biochemical diversity echoes the genetic diversity of the TP53 locus, which contains multiple genetic polymorphisms defining over 100 distinct TP53 haplotypes.5 Recently, an additional layer of regulatory mechanism has emerged through identification of p53 isoforms, which are physiological proteins expressed in normal cells from the TP53 gene owing to the use of alternative promoters, splicing sites and/or translational initiation sites.6, 7
The p53 isoforms were first identified in early studies investigating p53 expression patterns. In 1984, Matlashewski et al. cloned an N-terminal variant of the human p53 mRNA, whereas in 1985, Rotter and co-workers detected an alternatively spliced C-terminal variant of mouse p53, latter isolated in human cells.8, 9, 10 However, the ‘p53 isoform’ field has only really emerged in the past 10 years, when it became clear that TP53 retained the elaborate patterns of isoform expression that characterizes its homologues, TP63 and TP73. The rapid accumulation of descriptive, functional and clinical data on p53 isoforms has led to the emergence of a research community, which held its First International Meeting at the International Agency for Research on Cancer in Lyon, France, in September 2010.11 This review provides a brief survey of the ‘p53 isoforms’ field at a time when it is emerging at the forefront of p53 research.
TP63 and TP73: the Isoform Paradigm
The two p53-related proteins, p63 and p73, share strong structural, biochemical and biological homologies.12, 13 In particular, they bind specifically to DNA onto conserved p53 response elements (p53REs) by using their DNA-binding domain. In the late nineties, the cloning of TP63 and TP73 revealed an elaborate pattern of mRNA expression resulting in several protein isoforms.14, 15 Several N-terminal forms, produced by the use of alternative promoters and/or alternative splicing (i.e., TA forms, which contains the transactivation domain (TAD), versus ΔN forms, produced from an internal promoter resulting in the presence of a different TAD), were found combined with several C-terminal forms generated by alternative splicing (five for p63: α to ɛ; seven for p73: α to η).13 Far from being of minor component, the ΔNp63 and ΔNp73 forms are the major forms expressed in certain cell types.14, 16 In mice, knockout of the entire TP63 or TP73 loci (targeting all isoforms) revealed the roles of p63 and p73 in epithelial differentiation and neuronal development, respectively,15, 17 whereas no impact was observed in stem cell commitment.18 However, with the generation of isoform-specific knockout mice, a subtle interplay between the N-terminal isoforms has recently emerged, with the dynamic expression of N-terminal p63 or p73 isoforms appearing critical for maintaining the normal sequence of cell development (from stem to committed progenitors and then differentiated cells).19, 20 Building on this idea, Aberdam and co-workers analysed the impact of ΔNp63 and TAp63 isoforms in cellular commitment. In murine embryonic stem cells, ΔNp63, but not TAp63, is highly expressed during epidermal commitment and is critical for the expression of the cytokeratins K14 and K5, two markers of keratinocyte proliferation, indicating that only ΔNp63 is required for the commitment of ectodermal into epidermal cells.21, 22 Mills et al. observed that ΔNp63α overexpression in mouse embryonic fibroblasts (MEFs) resulted in the bypass of Ras-mediated senescence and enhanced carcinoma development in mice, suggesting that ΔNp63α inhibits senescence and therefore acts as an oncogene.23 By contrast, overexpression of TAp63 forms in p53−/− MEFs increased senescence and reduced tumour development in vivo, consistent with a p53-independent effect.24 Studying mice deficient for specific p73 protein isoforms, Mak, Melino and co-workers revealed that, like p63, the different p73 isoforms had dual cellular roles. In particular, TAp73−/− mice are spontaneously tumour-prone whereas ΔNp73−/− cells show impaired tumour formation in nude mice.25, 26 In addition to these cancer-related effects, knockout of either TAp73 or ΔNp73 isoforms resulted in isoform-specific defects in neurogenesis or neurodegeneration, respectively. Overall, these studies show that each N-terminal p63/p73 form has specific roles in regulating distinct cell differentiation pathways. Moreover, they demonstrate that the ΔN forms, by preventing senescence and maintaining progenitor cell status, may act as oncogenes, whereas TA forms, through their capacity to switch cells into a post-mitotic state, may act as tumour suppressors.
Generation of Human p53 Isoforms by Diverse Regulatory Mechanisms
Like TP63 and TP73, the human TP53 gene encodes several p53 protein isoforms through conserved mechanisms.6, 7 The main and most abundant p53 isoform is the canonical p53 protein, also termed TAp53α, as it contains an entire TAD and the longest C-terminal domain (Figure 1). In addition to the TA forms, three ΔN forms have been identified that differ by their translation initiation site. This is used to designate them as Δ40p53, Δ133p53 and Δ160p53. The four N-terminal p53 forms can be combined with three different C-terminal domains (α, β, γ). Recently, some cis- and trans-regulators have been identified as specific modulators of p53 isoform expression (Figure 1).
Δ40p53 expression: one form, several mechanisms
Compared with p53, the human N-terminal Δ40p53 forms lack the first 39 amino acids corresponding to the main TAD (Figure 1c). Matlashewski et al.27 identified a p53 mRNA species retaining the entire intron-2 (p53I2), indicating that an alternative splicing event leading to the retention of the TP53 intron-2 can occur (Figure 1b). They later observed that stop codons in intron-2 of the p53I2 mRNA prevent p53 expression from the first AUG.28 However, p53I2-transfected cells were found to express a 45-kDa protein, undetectable using antibodies recognizing the p53-TAD epitopes (DO1 or DO7), that corresponds to Δ40p53 initiated at a second AUG at codon-40, encompassed within a strong Kozak consensus.29 Alternative splicing of intron-2 can be regulated through G-quadruplex structures located in intron-3 of the p53 pre-mRNA.30 Using reporter assays and RNA–G-quadruplex ligands, it appears that G-quadruplex structures promote the correct splice-out of intron-2, leading to the fully spliced p53 (FSp53) mRNA encoding the full-length p53 protein; G-quadruplex disruption however favours the retention of intron-2 and thus p53I2 mRNA expression. This observation is the first clue that the TP53 sequence itself can modulate its own isoforms’ expression through regulation of alternative splicing.
In addition to alternative splicing, Δ40p53 forms can be encoded from the FSp53 mRNA through an internal initiation of translation at codon-40.29 Two internal ribosomal entry sequences (IRES) have been identified that regulate the translation of either p53 or Δ40p53 (Figure 1b).31, 32 However, the relative contribution, in vivo, of each of these mechanisms, which are producing Δ40p53, remains to be fully established.
Production of Δ133p53 and Δ160p53 forms from the internal P2 promoter
As for TP63 and TP73, TP53 contains an internal promoter that controls the expression of two N-terminal forms (Figure 1a).33 In addition to the proximal P1 promoter regulating p53 and Δ40p53 expression, an internal P2 promoter located between intron-1 and exon-5 regulates the transcription of p53 mRNAs initiated in intron-4 (p53I4) (Figure 1b).33 This internal P2 promoter is different from the P* promoter in TP53 intron-1 identified by Reisman et al.34, 35 that regulates the expression of an unrelated p53 transcript encoded by TP53 intron-1. Site-directed mutagenesis and siRNA methods revealed that translation of p53I4 mRNAs can be initiated at two distinct codons, AUG133 and AUG160, leading to the expression of the Δ133p53 and Δ160p53 proteins, respectively, that lack the TAD and part of the DNA-binding domain (Figure 1c).36 Surprisingly, Δ160p53 forms are expressed in K562 cells, which are considered as ‘p53-null’ cells. The TP53 mutation in K562 cells results in a premature stop codon between AUG133 and AUG160, thus preventing the expression of the TA, Δ40 and Δ133 forms without compromising the Δ160p53 reading frame. Thus, it should be kept in mind that some cells or tumours considered as ‘p53-null’ because of the presence of frameshift or nonsense mutations, may retain the capacity to express one or several p53 isoforms.29, 36
Experimental studies showed a p53-dependent regulation of Δ133p53 expression.37, 38 The internal P2 promoter contains p53REs located at the junction of exon-4/intron-4. Promoter deletion, site-directed mutagenesis and chromatin IP experiments demonstrate that the direct binding of p53 onto the p53REs results in an increased expression of both Δ133p53 mRNAs and the Δ133p53α protein. Some experimental evidence suggests that protein expression of Δ133p53α can be induced through p53-independent mechanisms. In particular, the accumulation of the Δ133p53α protein was observed in response to the knockdown of p68, a DEAD Box helicase involved in multiple transcriptional regulatory processes, and more recently in response to the expression of some p63 or p73 isoforms.39
The C-terminal spliced p53 forms, β and γ
The alternative splicing of intron-9 of human TP53 produces three different C-terminal p53 forms (α, β and γ) (Figure 1a).8, 33 Complete excision of intron-9 results in the expression of the α-forms corresponding to the classical p53 C-terminal domain (oligomerization domain, OD) (Figures 1b and c). On the other hand, partial retention of intron-9 generates the β- or γ-forms, in which the OD is replaced by 10 or 15 new amino acids, respectively. However, the mechanisms that control the alternative splicing of intron-9 are unknown.
The classical p53 C-terminal domain contains the main post-translational modification sites regulating p53 stability, such as the lysines residues ubiquitinated by Mdm2, an E3-ubiquitin ligase regulating p53 stability and activity.3 Their absence in β- and γ-forms led to the investigation as to whether the stability of the p53β and p53γ proteins is regulated by the Mdm2–ubiquitin–proteasome pathway. To date, results remain controversial. For instance, whereas constitutively overexpressed FLAG-tagged p53β or p53γ do not appear to interact with Mdm2 or to be degraded in a proteasome-dependent manner,40 Bourdon and co-workers observed that the endogenous p53β protein can be degraded by the proteasome in an Mdm2-dependent manner.
Biological Functions of Human p53 Isoforms
Based on early studies detailed below, the N-terminal isoforms lacking the TAD (i.e., Δ40p53, Δ133p53 and Δ160p53) were expected to only act as dominant-negative regulators of p53 activity. For instance, ectopic Δ40p53α expression downregulated the p53-induced transactivation on reporter genes and counteracted p53-dependent growth suppression in colony formation assays.28, 29 In addition, in human diploid fibroblast WI38 cells, expression of endogenous Δ40p53α increased during the G1/S transition, in parallel with decreased expression of p21.29 Furthermore, as Δ40p53α lacks the Mdm2-binding site, it escapes Mdm2-mediated degradation and does not accumulate in response to DNA damage, its expression persisting at low but stable amounts in many cell types.29, 41 These data support the notion that Δ40p53α inhibits basal p53 activities during cell-cycle progression. However, whether Δ40p53α can exert p53-independent effects is still unknown. Hainaut and co-workers observed that Δ40p53α contains an intact DNA-binding domain able to bind to p53REs in vitro. Thus, Δ40p53α may also exert intrinsic regulatory effects by competing with p53 for p53RE binding and thereby modulating their accessibility for other transcription factors, regulating the cell fate outcome depending upon cell type and cell context. In addition, Δ40p53α lacks the first TAD, which has been shown to be dispensable for p53 transcriptional activity, and retains the second TAD, which can regulate gene expression.42, 43 Therefore, with our current knowledge, one should be cautious in considering Δ40p53 as a simple dominant-negative inhibitor of p53.
Compared with Δ40p53α, the available data have clearly revealed that Δ133p53 controls p53 activity. In reporter assays, Δ133p53α can also behave as a dominant-negative inhibitor of p53.13, 33 Δ133p53α does not bind to consensus p53REs in vitro, consistent with its partial lack of the DNA-binding domain, and thus can also behave as a dominant mutant p53 protein.38 Experimental studies suggested that, instead of being a strict dominant inhibitor, Δ133p53α is instead a fine modulator of p53's suppressive activity as its expression determines cell fate in response to stress. The knockdown of Δ133p53α expression promotes p53-mediated apoptosis and G1 cell-cycle arrest in response to doxorubicin treatment, without altering the p53-dependent G2 cell-cycle arrest.37 These effects may be due to the ability of Δ133p53α to modulate gene expression in a promoter-dependent manner, as observed for p21WAF1, Mdm2 and Bcl-2.37 Interestingly, Δ133p53 silencing has also been associated with replicative, but not oncogene-induced, senescence in normal human fibroblasts through transcriptional regulation of p53-target genes, including p21WAF1 and mir-34a.44 Overall, these results are consistent with an oncogenic capacity of Δ133p53.
The biological functions of the C-terminal p53 isoforms (i.e., p53β and p53γ) remain poorly described and controversial. Bourdon et al.33 showed that, in the absence of stress, endogenous p53β bound to the Bax and p21WAF1 promoters, but only weakly to that of Mdm2. Moreover, in luciferase reporter assays in the absence of stress, the co-expression of p53β and p53 enhanced the p53 transcriptional activity on the p21WAF1 promoter but not on the Bax promoter, suggesting a promoter-dependent effect. These observations are consistent with the demonstration that p53β cooperates with p53 to accelerate senescence in human fibroblasts.44 By contrast, experimental studies failed to observe binding of FLAG-tagged p53β or p53γ onto p53RE consensus and to show a role of FLAG-tagged p53β or p53γ in p53-dependent apoptosis or senescence in cells constitutively overexpressing FLAG-tagged p53β or p53γ, and selected to grow in presence of neomycin.40 Thus, there is still debate on whether p53β or p53γ exert their activities in an autonomous manner or through an interaction with p53. Furthermore, there is no evidence of distinct biological activities for p53β or p53γ.
Overall, current experimental data on the biological roles of p53 isoforms are fragmented. Given that p53 isoforms differ from each other in the three functional domains (TAD, DNA-binding and OD), their potential to modulate p53-dependent responses is expected to be diverse and cell type-dependent. Further insight into which of these functions are of physiological or pathological relevance may come from animal model studies.
Animal Models: clues to the Physiological Significance of Isoforms
The simplest animal model to study p53 isoforms, Drosophila melanogaster
The diversification of the p53 gene family into three members occurred in vertebrates. Thus, invertebrates such as Drosophila contain a single p53-related gene, which encodes three protein isoforms (Figure 2): Dp53, corresponding to the human full-length p53; DΔNp53, a general counterpart of the human N-terminal p53 forms as it is encoded by an mRNA transcribed from an internal promoter (i.e., human Δ133) and contains a truncated TAD followed by a complete DBD and OD (i.e., human Δ40); and Dp53ΔC, encoded by a short transcript leading to a putative isoform bearing only the TAD.33, 45, 46, 47 The Drosophila p53 gene is activated by irradiation and exerts broad suppressive effects recapitulating those of the p53-family members, including regulation of apoptosis, aging, autophagy, differentiation and growth.48 Historically, DΔNp53 was the first form identified and previously termed Dp53; hence most of the functional studies to date have focused on the role of this particular isoform (Table 1).
Studies on the morphogenesis of imaginal discs have highlighted the role of DΔNp53 isoforms in the control of cell death.45, 47, 49, 50 In this system, DΔNp53 controls apoptosis through the Reaper–Hid–Grim (RHG) cascade. Indeed, irradiated imaginal discs from flies with a mutant Dp53 gene show reduced apoptosis but normal cell-cycle arrest, suggesting a specific regulatory role of the Dp53 gene product in apoptosis.45, 49 So far, the main isoform implicated in apoptosis appears to be DΔNp53, which directly regulates reaper (rpr) expression.45 However, DΔNp53 also exerts effects through other pathways. In particular, DΔNp53 may activate either apoptotic or non-apoptotic responses in photoreceptor cells depending on the cell differentiation status.50, 51, 52 Moreover, DΔNp53 appears to inhibit cellular differentiation in the retina independently of its apoptotic function.50
The Dp53 gene product also exerts important roles in controlling lifespan in a sex- and stage-dependent manner. When overexpressed in adult flies (Table 1), DΔNp53 limited lifespan in females and extended it in males. By contrast, when overexpressed during development, DΔNp53 exerted a similar dose-dependent effect on longevity in both sexes.53 Conversely, inactivation of the Dp53 locus increased lifespan in females but had only minor effects in males. Similar phenotypes were observed in Drosophila expressing dominant-negative Dp53 mutant transgenes or overexpressing DΔNp53, suggesting that DΔNp53 interferes with Dp53 activity.54 The role of Drosophila p53 has also been investigated in detail in other cellular functions such as DNA repair or compensatory proliferation.48, 55, 56 However, the specific roles of each Drosophila p53 isoform, Dp53 and DΔNp53, have not been studied in sufficient detail to understand the exact contributions of each isoform to development, stress responses or longevity.
Danio rerio, the historical model to study p53 isoforms
The zebrafish p53 protein, Zp53, recapitulates the suppressive and pro-apoptotic functions of human p53 upon genotoxic stress.57 So far, only N-terminal Zp53 isoforms have been identified (Figure 2): Zp53, corresponding to the human p53 protein;58 ZΔNp53, produced through an alternative splicing of intron-2 and thus similar to the human Δ40p53 forms;59 and ZΔ113p53, produced by an internal promoter located within the zebrafish Zp53 gene that is regulated by Zp53 itself, and thus equivalent to the human Δ133p53 isoform.37, 38, 60 In contrast to the human Δ40p53, AUG1 of ZΔNp53 is located within the partial intronic sequence retained by alternative splicing.59 Thus in ZΔNp53, the 38 N-terminal residues containing the TAD are replaced by 33 residues derived from the intron-2 sequence.
There is evidence that the ZΔNp53 transcript accumulates in response to γ-ray irradiation.59 In addition, ectopic expression of ZΔNp53 resulted in a strong developmental phenotype with hypoplasia and malformation of the head, eyes and somites (Table 1). This phenotype is dependent upon the presence of Zp53, the two isoforms forming a protein complex through their ODs. Overexpression of an OD-mutant ZΔNp53 or wild-type ZΔNp53 in a mutant Zp53 background is phenotypically ineffective. These observations suggest that ZΔNp53 exerts its effects by modulating the activity of Zp53 during zebrafish development.
ZΔ113p53 was discovered in a different context (Table 1). Cheng and co-workers found that in zebrafish embryos, loss of the Def gene (Digestive organ Expansion Factor) led to defects in the morphogenesis of digestive organs. In a genome-wide screen, they identified a shorter form of Zp53 whose expression was upregulated in def−/− embryos.60 Upregulation of ZΔ113p53 correlated with increased expression of p53-target genes involved in cell-cycle progression such as cyclin-G1 and p21WAF1, whereas pro-apoptotic genes such as Bax and Reprimo were not activated. Furthermore, ZΔ113p53 was found to selectively upregulate the Bcl-2L anti-apoptotic gene.61 This pattern of effects is consistent with the notion that ZΔ113p53, like Δ133p53, operates as a modulator of p53 in selectively activating defined target genes.
Mouse, the next generation of animal models
Six p53 isoforms have been described in mice, resulting from combination of three N-terminal p53 isoforms with two different C-terminal isoforms (Figure 2). In addition to the full-length mouse Mp53, Rotter and co-workers identified an alternative p53 mRNA retaining part of intron-10 that encodes a shorter isoform with new residues in place of the usual OD (Mp53AS), homologous to the human p53β.10, 62 Later, Mowat et al.63 isolated MΔ41p53, the mouse counterpart of human Δ40p53 forms. In addition, Khoury et al. isolated a shorter N-terminal form produced by an internal promoter within the mouse p53 gene, MΔ157p53, equivalent to the human Δ160p53 form. Khoury et al. also showed that MΔ41p53 and MΔ157p53 can be expressed as a C-terminal AS variant. As for the human p53 isoforms, Rotter and co-workers reported that Mp53AS modulates Mp53-mediated apoptosis and Mp53 transcriptional activity in luciferase reporter assays.64
In 2004, Maier et al.65 described a transgenic mouse overexpressing MΔ41p53 (Table 1). When expressed in a p53-null background, this isoform did not induce any particular phenotype. However, when expressed in a p53-competent background, an increased dosage of MΔ41p53 led to reduced size, accelerated aging and a shorter lifespan associated with hypo-insulinemia and glucose insufficiency.65, 66, 67 These effects were attributed to the hyper-activation of the insulin-like growth factor (IGF)-signalling axis by MΔ41p53, setting in motion a cascade that clamps unimpeded growth through p21.65 Furthermore, these MΔ41p53-overexpressing mice show cognitive decline and synaptic impairment early in life, also attributable to the hyper-activation of the IGF-1-signalling pathway.67 These observations are consistent with studies performed in vitro and in the zebrafish model, indicating that Δ40p53 isoforms may regulate growth suppression through modulation of p53 activity.
A premature aging phenotype was also reported in a non-physiological knock-in p53 mouse model expressing a ‘MΔ122p53’ form, truncated for the first 122 residues (Table 1).68 Although MΔ122p53 has no physiological equivalent in mouse, it can be considered as an ‘intermediate’ between the MΔ41p53 and MΔ157p53 isoforms, as it lacks the TAD and part of the DNA-binding domain. During adulthood, transgenic p53Δ122p53/Δ122p53 mice showed premature aging symptoms, such as balding and arthritis, similar to that observed in the p53+/m mice (deletion exon 1–6).68, 69 In addition, earlier tumour onset and shortened lifespan were observed in p53+/Δ122p53 mice as compared with p53+/− mice. Overall, these results support the hypothesis that mouse N-terminal p53 forms may operate as dominant oncogenes to promote cell proliferation and inflammation.
The studies summarized above highlight common themes in the ‘p53 isoform’ field. First, overall patterns of isoform expression are well-conserved throughout evolution. Second, N-terminal isoforms have a major role as regulators of physiological processes related to development, aging, lifespan and, possibly, carcinogenesis. In this respect, two key mechanisms are emerging. The Δ40p53 form exerts regulatory effects on signalling cascades controlled by p53, perhaps through direct interaction between the two isoforms. Conversely, isoforms corresponding to Δ133p53α modulate cell response by regulating gene expression in a p53-dependent and -independent manner. It should, however, be remembered that our current view of isoform activities remains fragmentary and that further studies are needed to better understand their roles and underlying mechanisms.
p53 Isoforms and Human Cancers
Genetic polymorphisms: effects on p53 isoform expression
The TP53 gene is highly polymorphic, with over 80% of known single-nucleotide polymorphisms (SNPs) located within introns or non-coding 5′ and 3′ sequences.70 Hainaut and co-workers showed that G-quadruplex structures, formed in intron-3 and regulating intron-2 splicing (and thus Δ40 form expression), overlap a common polymorphism, TP53 PIN3, which consists of a 16-bp duplication (A1, non-duplicated allele; A2, duplicated allele).30, 71 This polymorphism may therefore modulate the structure and/or the stability of the G-quadruplexes, and affect Δ40p53 expression (Table 2). Consistent with a functional effect, TP53 PIN3 has recently been identified as a strong genetic modifier of germline TP53 mutations. Indeed, carriers bearing A1A1 genotypes developed their first cancer on average 20 years earlier than carriers with an A1A2 genotype.72 This effect was detected in a Brazilian cohort, in which the p.R337H mutation of partial penetrance is very common owing to a widespread founder effect.5 It remains to be demonstrated whether a similar effect is observed in carriers of other TP53 mutation types.
Landi and co-workers have identified 11 different haplotypes defined by eight SNPs in a region from exon-3 to intron-4, overlapping part of the internal P2 promoter regulating Δ133p53 and Δ160p53 expression.73 Using these different haplotypes as promoters to drive luciferase expression, they found significant differences in basal promoter activity that were confirmed by analysing endogenous Δ133p53 expression in samples of normal colonic mucosa. Furthermore, in silico and in vitro DNA binding analyses suggested that an SNP in intron-4 (rs179287; C>T) affects proteins binding within the P2 promoter.73 These results suggest that genetic polymorphisms may modulate basal Δ133p53 expression. Whether these effects influence cancer risk remains to be demonstrated.
Mutations affecting the production of isoforms
Given the role of N-terminal p53 forms as inhibitors of p53 transactivation, it can be expected that overexpression of certain isoforms may represent an alternative to a mutation in TP53 for inactivating p53 in cancers, as observed in some small clinical studies.33, 74, 75 Analysis of the IARC TP53 database identified 1019 somatic cancer mutations that are predicted to disrupt the p53 coding sequence in the N-terminal region while leaving intact the sequence encoding at least Δ160p53. This represents 3.65% of all somatic mutations reported to date (version R15; www-p53.iarc.fr).70 Therefore, rare and/or silent mutations in TP53 may differentially affect the expression of p53 isoforms, as observed recently for mutations present in the IRES regulating the relative expression of p53 and Δ40p53 forms encoded by the full-length FSp53 mRNA.76 With respect to C-terminal isoforms, Hofstetter et al.77 have used an yeast-base functional assay and RT-PCR to show that mutations at splice sites of TP53 intron-9 can lead to aberrant expression of p53β mRNA in primary cultures of ovarian cancer cells. By contrast, somatic mutations at splice sites of introns 6 and 9 were found to generate spliced mutant p53 mRNAs (p53ζ, p53δ and p53ɛ), distinct from the physiological isoforms. However, the role of such mutant isoforms in carcinogenesis is not known.
Isoform expression and cancer outcomes
There is emerging evidence that p53 isoform expression is deregulated in human cancers (Table 2). In a study of 245 primary ovarian cancers, Hofstetter et al.77 observed that expression of p53β was associated with serous and poorly differentiated cancers, and, when expressed together with the functional p53 protein, it was correlated with poor recurrence-free and overall survival. By contrast, tumours expressing functional p53 and Δ40p53 showed improved recurrence-free survival of patients compared with tumours expressing no Δ40p53. Studies of patients with acute myeloid leukemia have shown that elevated expression of p53β and/or p53γ in blood cells was correlated with improved responses to chemotherapy,78 which may predict decreased chemoresistance and improved overall survival. An effect of isoform expression has also been observed on breast cancer prognosis. In a cohort of 127 breast cancer patients, Thompson and co-workers reported that patients whose tumours expressed both mutant p53 and p53γ mRNAs had a prognosis as good as patients whose tumours expressed a wild-type p53, suggesting that the expression of p53γ may abrogate the poor prognosis commonly associated with TP53 mutations.79
Conclusion
The field of ‘p53 isoforms’ is still in its infancy, but the increasing number of genetic, biochemical and clinical studies have clearly established that p53 isoforms are fundamental and important components of the p53 pathway. Data obtained from animal and cellular models indicate that p53 isoforms regulate the cell fate in response to developmental defects and cell damages by differentially regulating gene expression, both in a p53-dependent and -independent manner. Furthermore, the current data suggest that p53 isoforms have roles in all biological activities regulated by p53 (Figure 3). Therefore, one can reasonably expect that the characterization of the biochemical and biological activities of p53 isoforms will impact on the fields of cancer, embryo development and aging.
Future experiments will be needed to gain further insight into how p53 isoforms modulate the different biological activities. However, based on our current understanding of the p63, p73 and p53 isoforms, we have come to realize that the p53 pathway should no longer be considered as regulated by only the p53 protein, but by a set of p63, p73 and p53 isoforms that interplay with each other in regulating physiological functions. Further progress on this front will require the development of robust and standardized tools for the identification and quantification of p53, p63 and p73 protein isoform expression in experimental systems as well as in human tissues.
Abbreviations
- MEFs:
-
mouse embryonic fibroblasts
- TAD:
-
transactivation domain
- p53REs:
-
p53 response elements
References
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Acknowledgements
We thank all the speakers for their participation in the 1st International p53 Isoforms Meeting, and Michelle Wrisez for logistic help. The meeting was funded by IARC, the College of Medicine of the University of Dundee and the Laboratory of Molecular Biology of the Cell (UMR5239, CNRS at ENS de Lyon). Links: IARC TP53 database, http://www-p53.iarc.fr/; abstracts of the International p53 Isoforms Meeting, http://www-p53.iarc.fr/Download/Book_p53Isoforms2010_Final.pdf. VM is supported by a fellowship from the Breast Cancer Campaign; M-LD-D and BM by CNRS ATIP, Fondation pour la Recherche Médicale (FRM) and La Ligue contre le cancer; CS by a PhD grant from the French Ministry of Research; JH by INSERM and Institut Curie; PH and JH by l′Insititut National contre le Cancer (INCa, France; Projet Libre 2009, 2009-192); and J-CB by Cancer Research UK.
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Marcel, V., Dichtel-Danjoy, ML., Sagne, C. et al. Biological functions of p53 isoforms through evolution: lessons from animal and cellular models. Cell Death Differ 18, 1815–1824 (2011). https://doi.org/10.1038/cdd.2011.120
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DOI: https://doi.org/10.1038/cdd.2011.120
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