Abstract
Inhibitory PAS (Per/Arnt/Sim) domain protein (IPAS) is a dominant negative transcription factor that represses hypoxia-inducible factor 1 (HIF-1) activity. In this study, we show that IPAS also functions as a pro-apoptotic protein through binding to pro-survival Bcl-2 family members. In a previous paper, we reported that NF-κB-dependent IPAS induction by cobalt chloride repressed the hypoxic response in PC12 cells. We found that prolonged incubation under the same conditions caused apoptosis in PC12 cells. Repression of IPAS induction protected cells from apoptosis. Furthermore, knockdown of IPAS recovered cell viability. EGFP-IPAS protein was localized in both the nucleus and the cytoplasm, with a large fraction associated with mitochondria. Mitochondrial IPAS induced mitochondria depolarization and caspase-3 activation. Immunoprecipitation assays revealed that IPAS is associated with Bcl-xL, Bcl-w and Mcl-1. The association of IPAS with Bcl-xL was also observed in living cells by the FLIM-based FRET analysis, indicating direct binding between the two proteins. IPAS contributed to dysfunction of Bcl-xL by inhibiting the interaction of Bcl-xL with Bax. These results demonstrate that IPAS functions as a dual function protein involved in transcription repression and apoptosis.
Similar content being viewed by others
Main
Hypoxia-inducible factor 1 (HIF-1) is a transcription factor that functions as a master regulator of gene expression in response to hypoxia.1, 2, 3 HIF-1 consists of an oxygen-regulated HIF-1α subunit and a constitutively expressed Arnt (HIF-1β) subunit. In normoxia, HIF-1α is rapidly degraded by the ubiquitin–proteasome system. On the other hand, in hypoxia, HIF-1 is stabilized, translocated into the nucleus and bound to hypoxia-response element (HRE) to induce downstream gene expression of hypoxia-responsive proteins, such as erythropoietin, vascular endothelial growth factor and tyrosine hydroxylase. The inhibitory PAS (Per/Arnt/Sim) domain protein (IPAS), one of the alternatively spliced variants of HIF-3α, functions as a dominant negative inhibitor of HIF-1.4 IPAS directly interacts with HIF-1α and HIF-1α-like factor (HLF, also known as HIF-2α or EPAS1) and prevents their dimerization with Arnt and subsequent DNA binding. IPAS is predominantly expressed in the Purkinje cells of the cerebellum and corneal epithelium. In addition, it is reported that IPAS is induced by hypoxia in the heart and lung through activation of HIF-1.5 Thus, IPAS functions as a negative feedback inhibitor of HIF-dependent hypoxic response.
Hypoxic cell death can be mediated by HIF-1.6 It can induce apoptosis through activation of BNIP3 and Noxa, which were identified as HIF-1-responsive pro-apoptotic proteins.7, 8 However, HIF-1 also functions to prevent cell death and even stimulate cell proliferation.9 Constitutive HIF-1 stabilization is observed in many types of cancers, in which it is associated with resistance to hypoxic apoptosis.9 Recent papers have shown that some anti-apoptotic molecules are identified as HIF-1 targets, such as Bcl-xL and Mcl-1.10, 11 Although it is considered that a delicate balance determines cell fate between self-killing of cells by apoptosis via induction of pro-apoptotic factors and adaptation of cells to the hypoxic environment through induction of anti-apoptotic factors and cell growth-related proteins, the complex roles of HIF-1 in cell death and cell survival are not fully understood and remain to be elucidated.
Hypoxic cell death is influenced by the presence of reactive oxygen species (ROS). ROS are known to accumulate after ischemic/hypoxic reoxygenation or intermittent hypoxia leading to extensive cell death.12, 13 This situation is observed in several pathophysiological conditions such as cerebral and myocardial infarction.13, 14 Although the role of ROS in cell death is not well understood, they can attack and oxidize nucleic acids, proteins and lipids, resulting in neuronal apoptosis.13, 15
Rat pheochromocytoma-derived PC12 cells are widely used as in vitro model cells for investigating neuronal apoptosis in hypoxia/ischemic conditions.16, 17, 18, 19 There are several reports showing that cobalt chloride (CoCl2) or nitric oxide could mimic hypoxia/ischemic conditions, including generation of ROS, and induces mitochondria damage and apoptosis.16, 17, 18, 19, 20 Recent reports have shown that CoCl2-induced ROS leads to accumulation of HIF-1α in many mammalian cells, including PC12 cells.21, 22, 23 Moreover, our recent report showed that CoCl2-induced ROS also upregulates the expression of IPAS mRNA through NF-κB activation in PC12 cells.23 Although there is increasing evidence suggesting a close relationship between hypoxic cell death and ROS generation in hypoxia as described above, the underlying mechanism linking them is not fully understood.
Our analyses here demonstrate that IPAS functions as a pro-apoptotic factor acting in the mitochondria, although it is also localized in the nucleus. We also show that IPAS directly interacted with pro-survival Bcl-2 family members, such as Bcl-xL, and this binding led to dysfunction of Bcl-xL by blocking its anti-apoptotic activity to bind to Bax. These results demonstrate that IPAS functions as a dual function protein involved in gene regulation in response to hypoxia and hypoxic apoptosis.
Results
Apoptosis induced by CoCl2 in RPMI-cultured PC12 cells
To examine CoCl2-induced cell death in PC12 cells under different nutritional conditions, PC12 cells were cultured in DMEM or RPMI in the presence of CoCl2 for 24 h, and cell viability was measured by trypan blue exclusion. Figures 1a and b show that CoCl2-induced cell death in PC12 cells occurred when cultured in RPMI medium depending on the concentration of CoCl2, but not at all in DMEM. We have previously shown that IPAS was induced by CoCl2 in PC12 cells when cultured in RPMI but not in DMEM, and that addition of three nutrients, magnesium, histidine and threonine (3Mix) to RPMI abolished the induction.23 CoCl2-induced cell death in PC12 cells found in RPMI medium was similarly blocked by the addition of 3Mix (Figures 1a and b). Hereafter 150 μM CoCl2 was used to induce cell death because of its potent effect. To determine whether CoCl2-induced cell death of RPMI-cultured PC12 cells had the characteristics of apoptosis, we carried out DNA fragmentation assay and nuclear staining with Hoechst 33342. DNA fragmentation assay showed that a clear DNA ladder was observed 16 h after exposure of PC12 cells to CoCl2 (Figure 1c) only when cultured in RPMI. Nuclear condensation was detected in CoCl2-treated PC12 cells when cultured in RPMI, but not in DMEM or RPMI with 3Mix (Figure 1d). In addition, Annexin-V/propidium iodide (PI) staining showed that CoCl2 induced apoptosis rather than necrosis, and this cell death was inhibited by a caspase inhibitor, Z-VAD-FMK (Figure 1e). Furthermore, release of mitochondrial cytochrome c and activation of caspase-3 were observed in CoCl2-treated PC12 cells when cultured in RPMI (Figures 1f–h).
Induction of IPAS in RPMI-cultured PC12 cells and its role in induction of apoptosis
We have already shown that IPAS expression was significantly induced in response to CoCl2 only when PC12 cells were cultured in RPMI (Figure 2a).23 Therefore, we hypothesized that IPAS was one of the causal factors in CoCl2-induced apoptosis in RPMI-cultured PC12 cells, and we investigated this possibility by the treatment of PC12 cells with siRNA for IPAS. We have already shown that the endogenous IPAS mRNA level was decreased by IPAS/HIF-3α siRNA treatment (Figure 2b).23 Downregulation of HIF-3α expression by the treatment was hardly detectable as it was very weakly expressed (Figure 2b). In addition, ectopic expression of Myc-tagged mouse IPAS was greatly decreased following IPAS/HIF-3α siRNA treatment (Figure 2c). IPAS/HIF-3α siRNA treatment significantly recovered the cell viability of RPMI-cultured PC12 cells (Figure 2d) and decreased the genomic DNA fragmentation of PC12 cells exposed to CoCl2 (Figure 2e). Next, we examined the effect of siRNA treatment on mitochondrial membrane potential by JC-1 dye. In non-apoptotic cells, most of the JC-1 accumulated as aggregates in the mitochondria (red fluorescence) and only a small part of the JC-1 localized in the cytoplasm as a monomer (green fluorescence) as shown in Figure 2f. Green fluorescence of JC-1 was enhanced by CoCl2 treatment and the siRNA treatment inhibited this enhancement (Figure 2f). Furthermore, release of mitochondrial cytochrome c and activation of caspase-3 were inhibited in the cells by the siRNA treatment (Figures 2g and h). Immunoblot analysis also revealed that the siRNA treatment resulted in a significant reduction in the cleaved and active form of caspase-3 (Figure 2i).
Induction of mitochondrial clustering and nuclear condensation due to mitochondrial localization of IPAS
To examine the role of IPAS in CoCl2-induced apoptosis, we constructed a EGFP-tagged IPAS (IPAS WT) expression plasmid together with C-terminal deletion (IPAS N and N2) plasmids and two N-terminal deletion (IPAS C and IPAS C2) plasmids as shown in Figure 3a, and transfected them into PC12 cells (Figures 3 and 4), human embryonic kidney (HEK) 293T cells (Supplementary Figures 1, 2a and b) and Chinese hamster ovary (CHO)-K1 cells (Supplementary Figure 2c). MitoRed was used to reveal mitochondrial morphology. IPAS WT was localized in both the nucleus and cytoplasm with different nuclear to cytoplasmic ratios for each cell (Figures 3b and 4a and Supplementary Figures 1a and 2). Moreover, IPAS WT induced mitochondrial clustering near the nucleus and nuclear condensation of PC12 cells (Figures 3b and c) and HEK293T cells (Supplementary Figures 1a and b). Further investigation of the cytoplasmic localization with the caspase inhibitor using a confocal microscope showed that IPAS WT was localized in the mitochondria (Figure 4b). Mitochondrial clustering due to IPAS was diminished by the caspase inhibitor in PC12 cells (Figure 4b), indicating that this effect on mitochondrial morphology due to IPAS is downstream of caspase activation and is therefore a consequence of cell death and the condensation of the cell. Nuclear localization of IPAS WT was confirmed by colocalization of PI staining using a confocal microscope (Figure 4c). IPAS N was exclusively localized in the nucleus (Figures 3b, 4a and b, and Supplementary Figures 1a, 2a and b). IPAS N2 was mainly localized in the nucleus. On the other hand, IPAS C colocalized with MitoRed and strongly induced perinuclear mitochondrial clustering, especially in HEK293T cells. IPAS C2 was diffusely localized in the cytoplasm, partially colocalized with MitoRed and induced mitochondrial clustering to some extent (weaker in the effect than WT and C). IPAS C and C2 induced nuclear condensation of PC12 cells (Figure 3c) and HEK293T cells (Supplementary Figure 1b). IPAS N did not induce nuclear condensation. IPAS N2 induced nuclear abnormality to a much lower extent in PC12 cells.
Mitochondrial depolarization, release of cytochrome c and activation of caspase-3 induced by IPAS
To determine whether mitochondrial localization of IPAS initiates intracellular apoptosis signaling, we examined mitochondrial membrane potential by JC-1 fluorescent staining, release of cytochrome c and activation of caspase-3. When Cerulean-IPAS WT was expressed in PC12 cells, the percentage of cells exhibiting green fluorescence was enhanced (Figure 5a). When Cerulean-IPAS C was expressed, green fluorescence was observed in about 25% of PC12 cells (Figure 5a), suggesting that IPAS C was the more powerful inducer of mitochondrial depolarization. IPAS C2 induced mitochondrial depolarization to a similar extent as IPAS WT. IPAS N was unable to induce depolarization of mitochondria (Figure 5a). Next, we investigated release of cytochrome c in IPAS-expressed cells. IPAS WT, C and C2, but not IPAS N, induced release of cytochrome c in PC12 cells (Figure 5b). Ability of IPAS-deletion mutants to release cytochrome c was associated with their activity to induce depolarization of mitochondrial membranes. High levels of active caspase-3 were observed in the cells expressing IPAS WT, C and C2, whereas IPAS N was unable to activate caspase-3 (Figure 6 and Supplementary Figure 3).
Specific binding of IPAS to pro-survival Bcl-2 family proteins through its C-terminal region and inhibition of Bcl-xL/Bax complex formation by IPAS
The possibility was considered that IPAS might inhibit the function of Bcl-2 family proteins. To test this possibility, binding between IPAS and Bcl-2 family members was examined. Immunoprecipitation experiments in HEK293T cells showed that IPAS was able to bind to Bcl-xL, but not to Bcl-2 and Bax (Figure 7a). Similar results were obtained in the presence of Z-VAD-FMK (Supplementary Figure 4a). When Bcl-2 was highly expressed, a modest interaction with IPAS was observed (Supplementary Figure 4b). In addition, ectopically expressed IPAS was bound to endogenous Bcl-xL, but not to Bcl-2 and Bax (Figures 7b and c). Next, we examined whether IPAS binds to other pro-survival Bcl-2 family members. Figure 7d shows that IPAS bound to Bcl-xL, Bcl-w and Mcl-1 variant (Mcl-1V) with similar affinity, but not to Bcl-2 and A1a (also known as A1/Bfl-1). The C-terminal region of IPAS was found to interact with Bcl-xL whereas the N-terminal region did not interact with Bcl-xL (Figure 7e). This result accords well with the apoptotic activity of the C-terminal region (Figures 5 and 6 and Supplementary Figure 3). Although binding of IPAS C2 to Bcl-xL was examined, Myc-IPAS C2 was not detected in the cell lysate when Bcl-xL was co-expressed, possibly due to degradation because of its shorter tag. We investigated subcellular localization of IPAS and Bcl-xL using confocal fluorescent microscopy to examine their colocalization. Although Bcl-xL was not localized in the nucleus, it was colocalized with IPAS WT in the cytoplasm. IPAS C colocalized with Bcl-xL in the cytoplasm, particularly in the cluster of mitochondria (Figure 7f). Furthermore, we performed fluorescence resonance energy transfer (FRET) analysis between two fluorescent proteins using fluorescence lifetime imaging microscopy (FLIM) to examine the interaction between IPAS and Bcl-xL in living cells (Figures 7g and h). Lifetimes of IPAS-Cerulean were calculated to be 1.25 and 3.18 ns, with fraction ratios of 36.3 and 63.7%, respectively (Figure 7h). The fluorescence decay of IPAS-Cerulean was considerably accelerated in the presence of Bcl-xL-Citrine by energy transfer (Figures 7g left and h). Short and long lifetimes, τ1 and τ2, were reduced to 0.95 and 2.89 ns, respectively. This reduction in donor lifetimes was observed in the cytoplasm, but not in the nucleus, as shown in the FLIM map (Figure 7g bottom). On the other hand, homogeneous lifetimes were observed all over the cell when IPAS-Cerulean was expressed without Bcl-xL-Citrine. Corresponding to the reduction of donor fluorescence lifetimes, a fluorescence rise in the decay curve of Bcl-xL-Citrine was observed (data not shown), confirming the FRET. Similar results were obtained using a pair of IPAS-Cerulean and Citrine-Bcl-xL (Supplementary Figure 4c). Bcl-xL-Citrine was localized diffusely in the cytoplasm with a fraction associated with mitochondria probably because of C-terminal tag. FRET signals were detected in both cases, indicating that the interaction between IPAS and Bcl-xL is not dependent on their cytoplasmic localization. Next, the fluorescence decay of IPAS C-Cerulean was accelerated in the presence of Bcl-xL-Citrine by energy transfer (Figures 7g right and 7h). An N-terminal tag pair, Cerulean-Bcl-xL and Citrine-IPAS, did not exhibit the accelerated fluorescence decay (Supplementary Figure 4d), suggesting that the distance between the N-termini of IPAS and Bcl-xL is longer than that of the C-termini. These results demonstrate that the IPAS C-terminal domain directly binds to Bcl-xL and strongly suggest that it can trigger a series of events leading to activation of apoptosis. To confirm this possibility, we examined whether the interaction of IPAS with Bcl-xL inhibits the function of Bcl-xL to bind to Bax. Immunoprecipitation assays showed that IPAS inhibited the interaction of Bcl-xL with Bax by 60% (Figure 8a). We examined localization of endogenous Bax in PC12 cells treated with CoCl2. Figure 8b shows that Bax is predominantly cytosolic in resting cells and translocates into membrane fractions in response to CoCl2 treatment. Furthermore, we examined the effect of IPAS/HIF-3α siRNA treatment on binding of Bcl-xL with Bax in PC12 cells. Figure 8c shows that Bcl-xL formed a complex with Bax in resting cells and stimulation with CoCl2 resulted in the complete dissociation of the complex. IPAS/HIF-3α siRNA treatment inhibited this dissociation, suggesting that endogenous IPAS enhanced the dissociation of Bcl-xL/Bax complex in CoCl2-treated cells. Finally, we examined whether ectopic expression of Bcl-xL inhibits IPAS-mediated activation of caspase-3. As shown in Figure 8d, Bcl-xL strongly inhibited the activation of caspase-3. Bcl-2 inhibited this activation to some extent, probably because Bcl-2 weakly bound to IPAS and/or strongly bound to free Bax.
Binding of IPAS to HIF-1α and HLF
IPAS was originally identified as a negative regulator of HIF-1-mediated hypoxic response and is reported to directly bind to HIF-1α and HLF.4 We examined whether our IPAS constructs bind to HIF-1α and HLF. As expected, IPAS WT and IPAS N bound to HIF-1α and HLF in the co-immunoprecipitation assay (Supplementary Figures 5a and 6a). IPAS C also interacted with HIF-1α and HLF, with an intensity similar to IPAS N. When co-expressed with HIF-1α or HLF, most of the IPAS WT was colocalized with HIF-1α and HLF in the nucleus, particularly in the nuclear speckles (Supplementary Figures 5b and 6b). Similar colocalization of IPAS N with HIF-1α and HLF was observed in the nuclear speckles. Unexpectedly, HIF-1α and HLF were colocalized with IPAS C in the clusters of mitochondria. Binding affinity between IPAS WT and HIF-1α was compared with that between IPAS WT and Bcl-xL. IPAS WT bound to both Bcl-xL and HIF-1α, suggesting that similar affinity was present between the two interactions (Supplementary Figure 7). On the other hand, binding of IPAS C to HIF-1α was not observed when Bcl-xL was co-expressed. Similar results were obtained when HLF was used instead of HIF-1α. These results demonstrate that IPAS binds to HIF-1α and HLF using both the N-terminal and C-terminal regions and that the C-terminal region of IPAS has two functional regions. One acts as a region for binding to Bcl-xL and the other a region for binding to HIF-1α and HLF.
Discussion
Helix-loop-helix and PAS A domains that function as dimerization domains are localized in the N-terminal region of IPAS. On the other hand, no noticeable domains were reported in the C-terminal region. We determined that a specific sequence that is involved in the induction of apoptosis is localized in the C-terminal region, especially in the most C-terminal part. C-terminal 67 amino acids used for IPAS C2 constructs are encoded by exon 16 (final exon) and this exon is not used in other splicing variants of HIF-3α,5 suggesting that other splicing variants may have no pro-apoptotic activity. Figure 8e shows our present model for the action of IPAS on apoptosis. IPAS was able to bind to some of pro-survival Bcl-2 members, such as Bcl-xL through the C-terminal region on the mitochondria and may block the interaction between Bcl-xL and Bax, thus activating a Bax-dependent apoptotic cascade, followed by release of cytochrome c and activation of caspase-3. This study shows that the C-terminal region of IPAS is responsible for binding to Bcl-xL. Observations that interaction between Bcl-xL and Bax was decreased by overexpression of IPAS and increased by knockdown of IPAS support the model (Figures 8a and c). Finding that endogenous Bax was translocated to membrane fractions in response to CoCl2 treatment (Figure 8b) also supports the model. The mode of action of IPAS is reminiscent of BH3 only proteins. We surveyed sequences analogous to the BH3 domain within the C-terminal region, and found a region with very weak similarity to the BH3 domain. However, introduction of point mutations in the sequence did not inhibit the binding to Bcl-xL, suggesting that the binding mode of IPAS to pro-survival Bcl-2 members may be different from that of BH3 only proteins. Preferential binding of IPAS to Bcl-xL, Bcl-w and Mcl-1, but not Bcl-2 and A1 may be dependent on the distinctive binding mode of IPAS to pro-survival Bcl-2 members. Further experiments are required to characterize key sequences within IPAS necessary for binding to pro-survival Bcl-2 members.
Though we focused on the interaction of Bcl-xL with Bax, Bcl-xL is also known to bind to and inhibit activation of Apaf-1, a transmitter of apoptotic signals from mitochondrial damage to activate caspases.24 IPAS may also inhibit binding of Bcl-xL to Apaf-1 and other interacting proteins. Bcl-xL is critical for the survival of immature neurons, whereas Bax and Apaf-1 are crucial for neuronal cell death in the nervous system.25 Therefore, the functions of IPAS associated with these proteins may be important for neuronal cell death in vivo. We observed that IPAS was induced in the brain including substantia nigra and locus ceruleus in response to CoCl2, suggesting that IPAS expression may be related to neuronal cell death occurring in Parkinson’s disease and other neurodegenerative diseases. ROS seems to contain the driving force for neurodegeneration in Parkinson’s disease.15, 26 It is known that IPAS is predominantly expressed in the Purkinje cells of the cerebellum.4 However, it seems that these cells, despite IPAS expression, do not undergo apoptosis. One possible reason is that post-translational modification of IPAS occurs to avoid cell death in these cells. Although evidence for the modification of IPAS is presently not found, there are multiple consensus phosphorylation sites recognized by signal-regulated protein kinases in the IPAS sequence. This study demonstrates that IPAS was localized in nucleus rather than cytoplasm in HEK293T cells, and almost all IPAS was translocated to the nucleus when expressed with HIF-1α and HLF. Possible inhibitory proteins that interact with IPAS may forcibly sequester IPAS in the nucleus or other organelles to suppress its pro-apoptotic activity.
We confirmed that IPAS forms a complex with HIF-1α and HLF. The N-terminal region of IPAS exhibited binding activity to HIF-1α and HLF. It is plausible to assume that this association occurs through the bHLH (basic helix-loop-helix) and PAS domains present in the N-terminal region since the domains are used for a common dimerization interface in bHLH-PAS proteins. However, association of the C-terminal region with HIF-1α and HLF was unexpected. We believe that HIF-1α and HLF were accumulated in the mitochondria through a part of the IPAS C-terminal region (Supplementary Figures 6 and 7). Known-sequence motifs serving as protein interaction were not found in the region by a computer database search. It is necessary to delineate the regions that contributed to the binding in both the IPAS C-terminal region and the HIF-1α/HLF sequences.
Molecular mechanisms for switching from transcription factor to pro-apoptotic protein and vice versa are presently unknown, and elucidation of the mechanism will be needed for understanding of ROS-induced cell death.
Materials and Methods
Cell culture and DNA transfection
PC12 cells, HEK293T cells and CHO-K1 cells were obtained from the Cell Resource Center for Biomedical Research, Tohoku University, Sendai, Japan. PC12 cells were maintained in RPMI-1640 (Wako Pure Chemical Industries, Osaka, Japan) supplemented with 10% fetal bovine serum in collagen IV-coated dishes (BD Biosciences, San Jose, CA, USA) under 5% CO2 at 37°C, and transferred every three days. HEK293T cells and CHO-K1 cells were maintained as described.27, 28 PC12 and CHO-K1 cells were transfected using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. HEK293T cells were transfected using Lipofectamine 2000 and as described.27, 29
Cell viability assay with trypan blue dye exclusion
Cell number and viability were determined with a conventional hemocytometer by trypan blue dye exclusion. PC12 cells were plated in 6-well plate at 1.4 × 106 cells per well. After incubation for 24 h, cells were treated with 100 or 150 μM CoCl2 for 24 h in the indicated medium, and collected with 5 mM EDTA in Hank’s balanced salt solution (Invitrogen).
Determination of DNA fragmentation
PC12 cells were plated in collagen IV-coated 60 mm dish at 4 × 106 cells per dish. After incubation for 24 h, cells were treated with 150 μM CoCl2 for 16 h in the indicated medium, collected and lysed in 100 μl of lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM EDTA and 1% Triton X-100). After centrifugation at 1100 × g for 10 min, DNA was extracted from the supernatant and analyzed by agarose gel electrophoresis.
Nuclear staining with Hoechst 33342
PC12 cells were treated with 150 μM CoCl2 for 16 h in the indicated medium, stained with Hoechst 33342 for 30 min, fixed in 4% paraformaldehyde-PBS and mounted with Mowiol (Calbiochem, San Diego, CA, USA).
Annexin V/PI staining
Annexin V-FITC Apoptosis detection kit was purchased from B-bridge (San Jose, CA, USA). PC12 cells on glass coverslips were treated with or without 150 μM CoCl2 for 16 h in the indicated medium. The assays were carried out according to the manufacturer’s protocol. Cells were mounted in Mowiol and examined using a fluorescence microscope (Olympus IX71, Olympus, Tokyo, Japan). Caspase inhibitor, Z-VAD-FMK was purchased from Medical Biological Laboratories (Nagoya, Japan).
RT-PCR
PC12 cells were treated with or without 150 μM CoCl2 for 6 or 10 h in the indicated medium. Total RNA was extracted from cells using RNAiso (Takara Bio, Otsu, Japan). cDNAs were synthesized using oligo random hexamers (Invitrogen) and ReverTra Ace transcriptase (Toyobo, Osaka, Japan). A fraction (1 μl) of synthesized cDNA was amplified in a 20 μl reaction mixture containing 5 units of Ex Taq HS polymerase (Takara Bio). PCR cycles were chosen within the linear range of amplification. The PCR procedure consisted of 16 cycles of reaction for 18 S rRNA cDNA, 35 for IPAS and HIF-3α: 94°C for 30 s, 60°C for 30 s and 72°C for 20 s. The primers used for the reaction were described previously.23
siRNA
RNA oligonucleotides (21 nucleotides) homologous to mouse/rat HIF-3α/IPAS were designed as follows. Forward, 5′-GCUCAUUGGACACAGUAUCTT-3′; reverse, 5′-GAUACUGUGUCCAAUGAGCTT-3′. Control siRNA homologous to GFP sequence, forward, 5′-GGCUACGUCCAGGAGCGCATT-3′; reverse, 5′-UGCGCUCCUGGACGUAGCCTT-3′. PC12 cells were treated with annealed siRNAs by using Oligofectamine or Lipofectamine 2000 (Invitrogen) according to the manufacturer's protocol. At 24 h after transfection, cells were treated in the indicated medium with 150 μM CoCl2 for indicated times.
Molecular cloning and plasmid construction
Mouse IPAS cDNA was kindly provided by Dr. Y. Makino. All IPAS cDNA fragments were prepared by PCR and ligated into pEF-BOS vector containing three Myc tags, pEGFP-C1 vector or pCerulean-C1 vector. The full-length cDNAs of mouse Bcl-2, Bcl-xL, Bcl-w and Bax were synthesized by PCR using mouse brain cDNA derived from C57BL6J as a template. Mouse Mcl-1V and A1a were synthesized using mouse lung cDNA. Mcl-1V is a splicing variant of MCl-1 and lacks 46 amino acids residues within Mcl-1. These cDNAs were ligated into the pEF-BOS vectors containing three Myc, three FLAG or an HA tag. pBOS-FLAG human HIF-1α and pBOS-FLAG mouse HLF were described previously.30 The full-length cDNAs of HIF-1α and HLF were ligated into the pCitrine-C1 vector. All tags were added to the N-terminus of each protein. For FLIM-FRET analysis, IPAS, IPAS C and Bcl-xL were ligated into the pCerulean-N1 and pCitrine-N1 vector for a C-terminal tag, and pCerulean-C1 and pCitrine-C1 for an N-terminal tag.
Immunostaining
After fixation by 4% paraformaldehyde-PBS solution and permeabilization with 0.5% (or 0.1% for anti-cytochrome c) Triton X-100 in PBS for 5 min, cells were incubated with indicated primary antibodies at room temperature for 1 h, and then with the appropriate secondary antibodies at room temperature for 1 h. Cells were finally mounted in Mowiol and examined using a laser scanning confocal microscope (Olympus FV300) or a fluorescence microscope (Olympus IX71). An antibody against cytochrome c and active-caspase-3 was purchased from BD Pharmingen (San Jose, CA, USA) and Promega (Fitchburg, WI, USA), respectively. In PI staining, cells were treated by 100 μg/ml RNase at 37°C for 20 min after fixation by 4% paraformaldehyde-PBS solution, and incubated by 400 ng/ml PI for 10 min.
Determination of mitochondrial transmembrane potential with JC-1
PC12 cells were transfected with indicated constructs and treated with Z-VAD-FMK. 24 h after transfection, cells were incubated with JC-1 (Cayman Chemical, Ann Arbor, MI, USA) and Hoechst 33342 for 20 min at 37°C, and observed by using a fluorescence microscope (Olympus IX71).
Immunoprecipitation and immunoblotting
Immunoprecipitation assays were carried out as described previously.31 Cells were lysed in lysis buffer containing 20 mM Hepes (pH7.5), 100 mM NaCl, 1.5 mM MgCl2, 1 mM EGDA, 10 mM Na2P2O7, 10% glycerol, 1% Nonidet P-40, 1 mM dithiothreitol, 1 mM Na3VO4, 1 mM PMSF and 1% Aprotinin (Sigma, St. Louis, MO, USA). For Bax-Bcl-xL binding assay, cells were lysed in CHAPS buffer (PBS (pH 7.4) containing 10 mM CHAPS, 150 mM NaCl, 1 mM NaF, 10 mM Na-phosphate, 1 mM dithiothreitol, 1 mM Na3VO4, 1 mM PMSF and 1% Aprotinin) for 1 h on ice and centrifuged at 12 000 × g. The anti-Bcl-xL antibody was added to the supernatant of the CHAPS lysate and immunoprecipitates were analyzed. Binding reaction mixtures were loaded on 10∼13% SDS-polyacrylamide gels. After electrophoresis, the proteins were blotted onto a nitrocellulose membrane (GE Healthcare, Little Chalfont, UK) and probed with indicated first antibodies. Anti-Bcl-2 was purchased from Santa Cruz (Santa Cruz, CA, USA). Anti-Bcl-xL, anti-Bax and anti-caspase-3 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-Myc and anti-HA antibodies were purchased from Medical Biological Laboratories. Anti-FLAG and anti-β-tubulin was from Sigma. Horseradish peroxidase-linked goat anti-rabbit IgG (Jackson ImmunoReseach, West Grove, PA, USA) was used as the second antibody. The membrane was developed with the ECL Plus detection system (GE Healthcare).
FLIM measurements
FLIM measurements were carried out as described previously.28 In brief, fluorescence lifetimes of cells were measured on an inverted microscope (Zeiss (Jena, Germany): Axiovert 135, × 100 oil immersion objective with numerical aperture of 1.3) equipped with a disk anode microchannel plate photomultiplier (Europhoton, Berlin, Germany), which can detect photons in a time-resolved and space-resolved manner by using a time-correlated single-photon-counting technique. Fluorescence from live cell samples incubated at 37°C was sequentially collected at 475±25 nm for Cerulean and 575±25 nm for Citrine by bandpass filters at a count rate below about 0.5 counts (pixel·s)−1.
Subcellular fractionation
PC12 cells were lysed in lysis buffer containing 20 mM Hepes (pH7.5), 100 mM NaCl, 1.5 mM MgCl2, 1 mM EGDA, 10 mM Na2P2O7, 10% glycerol, 1 mM dithiothreitol, 1 mM Na3VO4, 1 mM PMSF and 1% Aprotinin. Cell lysates were vortexed for 15 s and centrifuged at 16 000 × g for 20 min. Pellets contained the enriched mitochondrial fraction, whereas supernatants contained mainly the cytosolic fractions. Pellets were re-lysed into lysis buffer shown above with Nonidet P-40. 20 μg of proteins were analyzed by immunoblotting with antibodies against Bax, Bcl-xL and GAPDH (Trevigen, Gaithersburg, MD, USA).
Statistical analysis
Data are given as mean±standard deviation, with the number of the experiments indicated. Statistical significance was established by Student’s t tests.
Abbreviations
- bHLH:
-
basic helix-loop-helix
- CHO:
-
Chinese hamster ovary
- CoCl2:
-
cobalt chloride
- FLIM:
-
fluorescence lifetime imaging microscopy
- FRET:
-
fluorescence resonance energy transfer
- HEK:
-
human embryonic kidney
- HIF-1:
-
hypoxia-inducible factor 1
- HLF:
-
HIF-1α-like factor
- HRE:
-
hypoxia-response element
- IPAS:
-
inhibitory PAS domain protein
- Mcl-1V:
-
Mcl-1 variant
- PAS:
-
Per/Arnt/Sim
- PI:
-
propidium iodide
- ROS:
-
reactive oxygen species
References
Hirota K, Semenza GL . Regulation of hypoxia-inducible factor 1 by prolyl and asparaginyl hydroxylases. Biochem Biophys Res Commun 2005; 338: 610–616.
Kaelin Jr WG . The von Hippel-Lindau protein, HIF hydroxylation, and oxygen sensing. Biochem Biophys Res Commun 2005; 338: 627–638.
Schofield CJ, Ratcliffe PJ . Signalling hypoxia by HIF hydroxylases. Biochem Biophys Res Commun 2005; 338: 617–626.
Makino Y, Cao R, Svensson K, Bertilsson G, Asman M, Tanaka H et al. Inhibitory PAS domain protein is a negative regulator of hypoxia-inducible gene expression. Nature 2001; 414: 550–554.
Makino Y, Uenishi R, Okamoto K, Isoe T, Hosono O, Tanaka H et al. Transcriptional up-regulation of inhibitory PAS domain protein gene expression by hypoxia-inducible factor 1 (HIF-1): a negative feedback regulatory circuit in HIF-1-mediated signaling in hypoxic cells. J Biol Chem 2007; 282: 14073–14082.
Carmeliet P, Dor Y, Herbert JM, Fukumura D, Brusselmans K, Dewerchin M et al. Role of HIF-1alpha in hypoxia-mediated apoptosis, cell proliferation and tumour angiogenesis. Nature 1998; 394: 485–490.
Bruick RK . Expression of the gene encoding the proapoptotic Nip3 protein is induced by hypoxia. Proc Natl Acad Sci USA 2000; 97: 9082–9087.
Kim JY, Ahn HJ, Ryu JH, Suk K, Park JH . BH3-only protein Noxa is a mediator of hypoxic cell death induced by hypoxia-inducible factor 1alpha. J Exp Med 2004; 199: 113–124.
Semenza GL . Targeting HIF-1 for cancer therapy. Nat Rev Cancer 2003; 3: 721–732.
Piret JP, Minet E, Cosse JP, Ninane N, Debacq C, Raes M et al. Hypoxia-inducible factor-1-dependent overexpression of myeloid cell factor-1 protects hypoxic cells against tert-butyl hydroperoxide-induced apoptosis. J Biol Chem 2005; 280: 9336–9344.
Chen N, Chen X, Huang R, Zeng H, Gong J, Meng W et al. BCL-xL is a target gene regulated by hypoxia-inducible factor-1 alpha. J Biol Chem 2009; 284: 10004–10012.
Xu W, Chi L, Row BW, Xu R, Ke Y, Xu B et al. Increased oxidative stress is associated with chronic intermittent hypoxia-mediated brain cortical neuronal cell apoptosis in a mouse model of sleep apnea. Neuroscience 2004; 126: 313–323.
Fujimura M, Tominaga T, Chan PH . Neuroprotective effect of an antioxidant in ischemic brain injury: involvement of neuronal apoptosis. Neurocrit Care 2005; 2: 59–66.
Lipton P . Ischemic cell death in brain neurons. Physiol Rev 1999; 79: 1431–1568.
Scherz-Shouval R, Elazar Z . ROS, mitochondria and the regulation of autophagy. Trends Cell Biol 2007; 17: 422–427.
Wang G, Hazra TK, Mitra S, Lee HM, Englander EW . Mitochondrial DNA damage and a hypoxic response are induced by CoCl2 in rat neuronal PC12 cells. Nucleic Acids Res 2000; 28: 2135–2140.
Jung JY, Kim WJ . Involvement of mitochondrial- and Fas-mediated dual mechanism in CoCl2-induced apoptosis of rat PC12 cells. Neurosci Lett 2004; 371: 85–90.
Jung JY, Han CR, Jeong YJ, Kim HJ, Lim HS, Lee KH et al. Epigallocatechin gallate inhibits nitric oxide-induced apoptosis in rat PC12 cells. Neurosci Lett 2007; 411: 222–227.
Jung JY, Roh KH, Jeong YJ, Kim SH, Lee EJ, Kim MS et al. Estradiol protects PC12 cells against CoCl2-induced apoptosis. Brain Res Bull 2008; 76: 579–585.
Araya J, Maruyama M, Inoue A, Fujita T, Kawahara J, Sassa K et al. Inhibition of proteasome activity is involved in cobalt-induced apoptosis of human alveolar macrophages. Am J Physiol Lung Cell Mol Physiol 2002; 283: L849–L858.
Chachami G, Simos G, Hatziefthimiou A, Bonanou S, Molyvdas PA, Paraskeva E . Cobalt induces hypoxia-inducible factor-1alpha expression in airway smooth muscle cells by a reactive oxygen species- and PI3K-dependent mechanism. Am J Respir Cell Mol Biol 2004; 31: 544–551.
Griguer CE, Oliva CR, Kelley EE, Giles GI, Lancaster Jr JR, Gillespie GY . Xanthine oxidase-dependent regulation of hypoxia-inducible factor in cancer cells. Cancer Res 2006; 66: 2257–2263.
Torii S, Kobayashi K, Takahashi M, Katahira K, Goryo K, Matsushita N et al. Magnesium deficiency causes loss of response to intermittent hypoxia in paraganglion cells. J Biol Chem 2009; 284: 19077–19089.
Hu Y, Benedict MA, Wu D, Inohara N, Nunez G . Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation. Proc Natl Acad Sci USA 1998; 95: 4386–4391.
Yuan J, Yankner BA . Apoptosis in the nervous system. Nature 2000; 407: 802–809.
Shen J, Cookson MR . Mitochondria and dopamine: new insights into recessive parkinsonism. Neuron 2004; 43: 301–304.
Yasumoto K, Kowata Y, Yoshida A, Torii S, Sogawa K . Role of the intracellular localization of HIF-prolyl hydroxylases. Biochim Biophys Acta 2009; 1793: 792–797.
Tomokuni Y, Goryo K, Katsura A, Torii S, Yasumoto K, Kemnitz K et al. Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy in living cells. Febs J 2010; 277: 1310–1318.
Carpentier E, Paris S, Kamen AA, Durocher Y . Limiting factors governing protein expression following polyethylenimine-mediated gene transfer in HEK293-EBNA1 cells. J Biotechnol 2007; 128: 268–280.
Ema M, Hirota K, Mimura J, Abe H, Yodoi J, Sogawa K et al. Molecular mechanisms of transcription activation by HLF and HIF1alpha in response to hypoxia: their stabilization and redox signal-induced interaction with CBP/p300. Embo J 1999; 18: 1905–1914.
Torii S, Kusakabe M, Yamamoto T, Maekawa M, Nishida E . Sef is a spatial regulator for Ras/MAP kinase signaling. Dev Cell 2004; 7: 33–44.
Acknowledgements
We are grateful to Dr. Y Makino for the gift of mouse IPAS cDNA. We also thank K Saka, N Tajikawa and A Suzuki for technical assistance.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no conflict of interest.
Additional information
Edited by V De Laurenzi
Supplementary Information accompanies the paper on Cell Death and Differentiation website
Supplementary information
Rights and permissions
About this article
Cite this article
Torii, S., Goto, Y., Ishizawa, T. et al. Pro-apoptotic activity of inhibitory PAS domain protein (IPAS), a negative regulator of HIF-1, through binding to pro-survival Bcl-2 family proteins. Cell Death Differ 18, 1711–1725 (2011). https://doi.org/10.1038/cdd.2011.47
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/cdd.2011.47
Keywords
This article is cited by
-
Cobalt chloride postconditioning as myoprotective therapy in cardiac ischemia–reperfusion
Pflügers Archiv - European Journal of Physiology (2022)
-
Attenuation of inhibitory PAS domain protein-induced cell death by synthetic peptides derived from Mcl-1 transmenbrane domain
Cell Death Discovery (2021)
-
Direct protein–protein interaction between Npas4 and IPAS mutually inhibits their critical roles in neuronal cell survival and death
Cell Death Discovery (2021)
-
Inhibitory PAS domain protein is a substrate of PINK1 and Parkin and mediates cell death in a Parkinson’s disease model
Cell Death & Disease (2015)
-
Involvement of inhibitory PAS domain protein in neuronal cell death in Parkinson’s disease
Cell Death Discovery (2015)