Abstract
Alkylating DNA-damage agents such as N-methyl-N′-nitro-N′-nitrosoguanidine (MNNG) trigger necroptosis, a newly defined form of programmed cell death (PCD) managed by receptor interacting protein kinases. This caspase-independent mode of cell death involves the sequential activation of poly(ADP-ribose) polymerase-1 (PARP-1), calpains, BAX and AIF, which redistributes from mitochondria to the nucleus to promote chromatinolysis. We have previously demonstrated that the BAX-mediated mitochondrial release of AIF is a critical step in MNNG-mediated necroptosis. However, the mechanism regulating BAX activation in this PCD is poorly understood. Employing mouse embryonic knockout cells, we reveal that BID controls BAX activation in AIF-mediated necroptosis. Indeed, BID is a link between calpains and BAX in this mode of cell death. Therefore, even if PARP-1 and calpains are activated after MNNG treatment, BID genetic ablation abolishes both BAX activation and necroptosis. These PCD defects are reversed by reintroducing the BID-wt cDNA into the BID−/− cells. We also demonstrate that, after MNNG treatment, BID is directly processed into tBID by calpains. In this way, calpain non-cleavable BID proteins (BID-G70A or BID-Δ68-71) are unable to promote BAX activation and necroptosis. Once processed, tBID localizes in the mitochondria of MNNG-treated cells, where it can facilitate BAX activation and PCD. Altogether, our data reveal that, as in caspase-dependent apoptosis, BH3-only proteins are key regulators of caspase-independent necroptosis.
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Main
When and how a cell dies is one of the essential questions to understand the biology of the cell. A cell is considered dead when the integrity of its plasma membrane is compromised, when its fragments are engulfed by an adjacent or a professional cell or when its components, including the nucleus, are fragmented. It is more complicated to outline how a cell could die. Historically, two major types of cell death have been distinguished: apoptosis, a controlled or programmed cell death (PCD), and necrosis, an uncontrolled or accidental death.1 Cell biologists have particularly focused their attention on apoptotic PCD, helping to characterize it at both genetic and biochemical levels.2 Fascinatingly, the use of apoptotic inhibitors or cells that are deficient in key apoptotic molecules has revealed the existence of new PCD pathways. As a consequence, active forms of cell death started to be referred to as caspase-dependent or caspase-independent.3 More recently, it has been accepted that necrosis is more than an unregulated type of death. Indeed, a cell can use different mechanisms/pathways with underlying apoptotic or necrotic features to accomplish its proper demise.4
The analysis of the tumor necrosis factor (TNF) signaling path has shed new light on the existence of PCD pathways with necrotic features. When TNF binds its receptor, two ways can be engaged: (i) caspase-dependent apoptosis, mediated by activation of caspase-8, and (ii) something similar to necrosis, which is caspase-independent, and is mediated by receptor interacting protein (RIP) kinases, RIP1 and RIP3. Pharmacological and genetic approaches have revealed a panoply of modulators of TNF-induced necroptosis, a term used to define this finely regulated form of cell death.5 Strikingly, PCD by necroptosis (programmed necrosis) is also induced by high doses of the alkylating DNA-damage agent N-methyl-N′-nitro-N′-nitrosoguanidine (MNNG).6 This necroptotic process, which is also regulated by the kinase RIP1,7 is executed by the harmonic activation of poly(ADP-ribose) polymerase-1 (PARP-1), Ca2+-dependent calpain Cys-proteases, and the pro-apoptotic BCL-2 member BAX.8 A key consequence of the activation of these three enzymes is the mitochondrial release of truncated AIF (tAIF), a major effector in caspase-independent PCD.9, 10, 11, 12, 13 Cytosolic tAIF rapidly redistributes to the nuclear compartment where, assisted by γH2AX and cyclophilin A (CypA), it promotes chromatinolysis and cell-viability loss.14, 15
Among the features of MNNG-induced necroptosis, the implication of BCL-2 family members appears as a milestone. The BCL-2 set of proteins is distributed in three subgroups according to their properties.16 The first subfamily comprises BCL-XL, BCL-2, BCL-W and MCL-1. They contain three or four BCL-2 homology (BH) domains that are known to be necessary for their anti-apoptotic function. Through their interactions with other BCL-2 members, they negatively regulate the mitochondrial release of pro-apoptotic proteins.16 The second subgroup corresponds to pro-apoptotic proteins, such as BAX and BAK, which are able to form pores or associate with pore-forming proteins in the outer mitochondrial membrane. This process induces mitochondrial permeabilization and the release of cell death-promoting proteins.17 The third BCL-2 subfamily regroups the proteins that only have a short BH3 domain. These BH3-only proteins interact with both anti- and pro-apoptotic BCL-2 members to induce PCD.16
In MNNG-induced necroptosis, the key role of the two first subgroups of the BCL-2 family has been recently established: BCL-2 and BAX manage the mitochondrial release of tAIF.8 However, a pivotal question remains: How is BAX activated here? With the help of a panel of murine embryonic fibroblast (MEF) knockout cells, the aim of the present work was to examine this issue. This approach revealed that, through its pro-apoptotic function, BID regulates BAX activation and PCD. Cleaved into tBID via a calpain cleavage at Gly70, this BH3-only protein is indeed the link between calpains and BAX in MNNG-mediated necroptosis.
Results
MNNG-induced caspase-independent necroptosis: a highly regulated PCD process
Treatment of MEFs with MNNG induces necroptosis, a RIP1 kinase-dependent mode of PCD modulated by necrostatin-1 (Supplementary Figure 1).5, 6, 7 This type of cell death, enabled here by the action of PARP-1, is a caspase-independent process with significant alterations detected as soon as 6 h of MNNG treatment (Figure 1a).8, 14 As in staurosporine (STS)-induced caspase-dependent apoptosis (our positive control), MNNG-treated cells undergo Annexin V-detectable phosphatidylserine (PS) exposure. However, in contrast to STS-induced PCD, MNNG treatment provokes a double Annexin V/PI(propidium iodide)-positive labeling (Figure 1a), which is indeed a hallmark of this type of PCD.18 MNNG-induced death is also characterized by the sequential activation of calpains and BAX (Figures 1b and c).8 Once in mitochondria, BAX provokes a mitochondrial damage that is accompanied by the release of tAIF, the truncated form of AIF, to the cytosol and nucleus (Figures 1d and e).8, 14 In the nucleus, this apoptogenic protein generates TUNEL-detectable 3′-OH DNA breaks in DNA (Figure 1f). Altogether, these events represent the biochemical hallmarks of MNNG-mediated necroptosis.8, 14
BAX activation is critical in MNNG-induced necroptosis
The presence of tAIF in extramitochondrial compartments is vital in the PCD process triggered by MNNG. As demonstrated by BAX genetic ablation, tAIF release and necroptosis are specifically controlled by the mitochondrial action of activated BAX (Figures 2a and b).8 But, how is BAX activated in necroptosis? We have previously described that BAX activation depends on calpain activity.8 Thus, a first possibility is that calpains cleave inactive BAX at Asp33 (D33) to yield a p18 active protein.19 By an immunoblotting approach, we thus tested whether the treatment of MEFs with MNNG generated the p18 active form of BAX. As depicted in Figure 2c, in contrast to STS, BAX does not become cleaved at D33 even 9 h after MNNG treatment. This result indicates that, in MNNG-induced necroptosis, BAX is not directly processed by calpains.
BID, but not BIM or BAD, is necessary for MNNG-induced BAX activation
The negative results represented in Figure 2c led us to analyze other possibilities for calpain-mediated BAX activation. Two manuscripts have already described that calpains control JNK1 activation20 and that AIF is released from mitochondria by JNK1-mediated activation.7 Moreover, it has been demonstrated that JNK phosphorylation and activation of the BH3-only proteins, BIM and BAD, couple the stress-activated signaling pathway to the BAX-dependent cell death machinery.21, 22 Thus, a working hypothesis is that calpains activate JNK1 which, by means of BIM or BAD phosphorylation, yields active BAX. A third BH3-only protein, BID, could also be implicated in calpain-mediated BAX activation. Indeed, calpains cleave BID in a 15-kDa fragment similar to the pro-apoptotic caspase-cleaved tBID.23 Once generated, tBID could provoke mitochondrial AIF release.13, 24, 25 Thus, a second BH3-only working hypothesis is that calpains cleave BID into tBID. Truncated BID could then regulate BAX activation, which provokes mitochondrial tAIF release and necroptosis.
We assessed the potential role of the BH3-only proteins BIM, BAD, and BID in MNNG-induced PCD by means of gene knockout MEFs. When comparing with WT, we observed that in BIM−/− or BAD−/− cells, both MNNG-induced necroptosis and BAX activation remained unaffected. In contrast, MNNG-induced BAX activation and death were efficiently delayed in BID−/− MEFs (Figures 3a–c). In these cells, the percentage of PS exposure and loss of viability reached ∼10% 9 h after MNNG treatment. At the same time, MNNG-induced death in about 60% of WT cells. As expected from the absence of BAX activation in BID−/− MEFs (Figure 3c), the presence of tAIF in extramitochondrial compartments was negative (Figure 3d). As internal control, we confirmed that WT, BIM−/−, BAD−/−, and BID−/− cells treated with the caspase-dependent apoptosis inducer STS displayed similar cell death levels (Figure 3a), confirming that these MEFs remained sensitive to other PCD inducers. In any case, BID and not BIM or BAD deficiency disabled BAX activation and necroptosis.
By reintroducing BID-wt cDNA into BID−/− cells by lentiviral transduction, we corroborated that the loss of the BAX-dependent necroptotic hallmarks was due to the specific lack of BID. Indeed, BID-wt cDNA transduction fully resensitized BID−/− cells to MNNG-mediated PCD, including mitochondrial damage, BAX activation, TUNEL positivity, and cell-viability loss (Figures 4a–d).
The BAX-dependent necroptotic hallmarks are abolished in MNNG-treated BID−/− MEFs
To gain insights into the role of BID in the MNNG-mediated PCD pathway, we next determined whether the absence of BID exclusively affected BAX activation or if, on the contrary, BID deficiency disabled other key necroptotic events. We first verified whether PARP-1 and calpains, which act upstream of BAX in MNNG-mediated PCD, were activated or not in BID−/− MEFs. PARP-1 activation is assessed by the inverse relationship existing between poly(ADP-ribose) (PAR) and NAD+ and ATP cellular levels. Note that we have previously demonstrated that the specific activation of PARP-1 in MNNG-mediated necroptosis is responsible for both the synthesis of the PAR polymers observed and the depletion of NAD+ and ATP cellular pools.8 As shown by the formation of PAR polymers, PARP-1 was equally activated in WT and BID−/− MEFs (Figures 5a and b). This demonstrated that, even in the absence of BID, PARP-1 is an active enzyme. In line with that, MNNG caused a rapid and similar decrease in NAD+ and ATP cellular levels in both WT and BID−/− MEFs (Figures 5c and d). The specificity of the PARP-1 dependent NAD+ loss associated with MNNG treatment in these cells was confirmed using a pharmacological PARP inhibitor, PJ34. Meanwhile, calpain activity was measured with the help of a cell-permeable substrate that reports calpain activity in live cells. This approach showed that MNNG induced rapid calpain activation in WT and BID−/− MEFs (Figure 5e). Overall, these results indicate that BID−/− MNNG-treated MEFs activate PARP-1 and calpains in exactly the same way as WT cells. In contrast, MNNG-treated BID−/− MEFs never present BAX-dependent necroptotic hallmarks: ΔΨm drop, tAIF release, and TUNEL positivity (Figures 5f–h).
These data raised an additional question: How can MNNG-treated BID−/− MEFs be viable with activated PARP-1/calpains, consumed NAD+/ATP pools, and massive DNA damage (assessed by a COMET assay, Supplementary Figure 2A)? To answer this question, we performed long-term experiments in MNNG-treated WT and BID−/− MEFs. As depicted in Supplementary Figure 2b, at 12 h post MNNG treatment, 98% of WT MEFs were necroptotic. In contrast, only ∼20% of BID−/− cells exposed the characteristic double Annexin V/PI-positive labeling. Strikingly, a significant population of BID−/− MEFs (∼45/50%) remained viable even 24 h post MNNG incubation (Supplementary Figures 2B and C). The rest of the BID−/− population exploded (Supplementary Figure 2D). Overtime, similarly to what has been previously reported for MNNG-treated H2AX−/− MEFs,14 it seems that the cell is unable to achieve the necroptotic PCD program in the absence of BID. As a result, the ‘highly damaged’ cell, which is unable to proliferate/divide (Supplementary Figure 3), explodes by an uncontrolled form of necrosis.
Altogether, our results identify BID as a key component of MNNG-induced necroptosis. By controlling BAX activation, BID manages this type of PCD.
MNNG-induced DNA damage and necroptosis is not influenced by the pro-survival function of BID
BID is not only a pivotal executioner of PCD. In response to a DNA double-strand breaks (DSB) damage such as that triggered by etoposide, BID activates a pro-survival cell cycle regulatory activity mediated by its phosphorylation at Ser61 and 78 (S61 and S78).26, 27 Thus, to achieve the characterization of the role of BID in MNNG-induced DNA damage, which also provokes DSB in DNA,14 we evaluated the relevance of the pro-survival role of BID. Specific immunoblotting detection successfully revealed a time-dependent BID phosphorylation in MNNG-treated cells (Supplementary Figure 3A), suggesting that the pro-survival role of BID was enabled. We explored this possibility by a cell cycle analysis performed in MNNG-treated WT and BID−/− cells. Surprisingly, contrary to what had been previously reported for etoposide,26 the percentages of the different cell cycle phases remained similar before and after MNNG treatment in both cell types (Supplementary Figures 3B, C and 4). This result opens two possibilities: (i) MNNG treatment causes the arrest of the cell cycle or (ii) this alkylating agent does not alter the cellular cycling at all. We verified these two alternatives by a BrdU/PI assessment. As revealed by the absence of proliferating cells, treatment with MNNG provoked the cell cycle halt in both WT and BID−/− MEFs (Supplementary Figure 3D). Altogether, our results differentiate the response of BID−/− cells to MNNG and etoposide, and suggest that necroptosis is not modulated by the cell cycle/DNA repair mechanisms implicating phosphorylated BID. This is substantiated by a flow cytometry assessment performed in BID−/− MEFs stably transfected with BID-wt or a non-phosphorylatable BID-S61A/S78A cDNA (Ser61 and 78 of BID-wt were mutated to Ala). Our data show that, contrary to what has been reported after etoposide DSB generation, MNNG does not induce a different rate of necroptosis in BID−/− cells expressing BID-S61A/S78A or BID−/− cells expressing BID-wt (Supplementary Figure 3E). Therefore, we conclude that the phosphorylation of BID does not regulate the response of MEFs to the DNA damage provoked by MNNG. This necroptosis is exclusively modulated by the apoptogenic function of BID.
BID is cleaved into tBID by calpains in MNNG-mediated necroptosis
As indicated above, the pro-apoptotic properties of the BH3-only protein, BID, are pivotal in the BAX activation that controls caspase-independent necroptosis. In apoptotic caspase-dependent PCD, this apoptogenic action is generally arbitrated by the caspase-cleaved BID, tBID.28, 29 In apoptotic PCD, tBID has a double role in BAX activation. On the one hand, tBID opens the N-terminal moiety of BAX.30 On the other hand, tBID redistributes to mitochondria where it favors BAX recruitment and activation.16, 31, 32 Hence, it seems mandatory to verify whether, in MNNG-treated MEFs, BID exerts its BAX activation function by means of the truncated form. As indicated in Figure 6a, a kinetic immunoblot analysis performed with a specific antibody demonstrated that a fraction of BID became cleaved into tBID after MNNG treatment. The time-dependent tBID generation correlated to MNNG-induced necroptosis (Figure 1a). Moreover, as was to be expected, tBID localized in mitochondria, where it was able to achieve its BAX-activating function (Figure 6a, right panels).
How does BID become activated/cleaved in caspase-independent necroptosis? Are calpains implicated in this cleavage? To answer these questions, we first tested whether BID was cleaved in calpain-inactivated knockout MEFs (CAPN4−/−).20 As depicted in Figure 6b, MNNG-induced BID cleavage was abolished in CAPN4−/− cells. Note that these cells treated with STS displayed similar levels of tBID to WT cells. This confirmed that BID could be caspase-dependent cleaved in CAPN4−/− MEFs. Interestingly, as could be expected from the absence of tBID in MNNG-treated CAPN4−/− MEFs, BAX activation, ΔΨm drop, PS exposure, and cell-viability loss were also negative (Figures 6c–e). These results place BID between calpains and BAX in this mode of death and indicate that calpains are implicated in the tBID generation associated to MNNG-mediated PCD.
Indeed, is BID directly cleaved into tBID by calpains or is tBID generated by a protease downstream of calpains? To fully analyze these two possibilities we assessed whether calpain non-cleavable (nc) BID restored MNNG-mediated necroptosis in BID−/− MEFs. The reactivation of MNNG-mediated necroptosis in these cells would indicate that a protease other than calpains generates the processed fragment of BID. The opposite result (calpain ncBID is not processed into tBID after MNNG treatment and is unable to restore PCD) would indicate that BID is directly cleaved into tBID by calpains to promote BAX activation. To design the calpain ncBID vectors, we took into consideration theoretical sequential/structural determinants of calpain-mediated cleavage33 and previous work suggesting that calpains processed BID into tBID at position Gly70.34 Thus, we generated two constructs: BID-G70A, which contains the Gly70 mutated to Ala, and BID-Δ68–71, in which the entire ‘calpain-cleavable region of BID’ has been deleted. Note that, similarly to the caspase-mediated BID cleavage occurring at D59 position,28 the cleavage at G70 provoked the removal of the BID N-terminus and exposed the amphipathic α helix BH3 on the active C-terminal tBID p15 fragment (Figure 7a). As illustrated in Figures 7b–e and Supplementary Figure 5, transduction of BID−/− MEFs with lentiviruses carrying vectors of BID-wt, a caspase ncBID-D59A mutant,35 or the two calpain ncBID mutants defined above revealed that: (i) BID-wt and BID-D59A resensitized BID−/− cells to MNNG-induced necroptosis. This confirmed our results showing that AIF-mediated necroptosis is a caspase-independent mode of PCD promoted by BID; (ii) BID-G70A or BID-Δ68-71 transduction does not restore the MNNG ability to induce necroptosis in BID−/− MEFs; and (iii) as was to be expected, BID-wt and BID-D59A are processed after MNNG treatment. On the contrary, BID-G70A and BID-Δ68-71 remain as precursor proteins. Note that BID−/− cells transduced with calpain ncBID mutants retain the apoptotic function (Supplementary Figure 6). Altogether, these data reveal that, in AIF-mediated necroptosis, calpains cleave BID into tBID at Gly70. The mutation/deletion of the calpain cleavage site of BID is sufficient to prevent BID processing, BAX activation, mitochondrial damage, and cell-viability loss.
Discussion
The present work uncovers a milestone in understanding the molecular mechanisms that regulate the necroptotic pathway induced by MNNG: the pivotal role of the BH3-only protein, BID. Together with our previous results8, 14 our present data provide a detailed sequence of events for DNA damage-mediated necroptosis: MNNG treatment induces a PARP-1 hyperactivity that leads to calpain activation. Calpains generate the cleaved form of BID (tBID), which redistributes from the cytosol to mitochondria, where it regulates BAX activation. Once activated, BAX provokes mitochondrial damage and tAIF mitochondrial release. tAIF relocalizes to the nucleus and, associated to γH2AX and CypA, induces chromatinolysis and cell-viability loss (Figure 8).
This work strengthens the idea that necroptosis is underpinned by a paradoxal PCD program, which is different from but similar to the broadly studied apoptotic program. It is different from apoptosis in as much as necroptosis substitutes caspases for calpains, enables a specific DNA-degrading module, and generates a controlled double Annexin V/PI labeling.18 It is similar to apoptosis because mitochondria has a pivotal role and operates under the control of typical apoptotic molecules (e.g., PARP-1, BID, BCL-2, BAX). The apoptotic and the necroptotic pathways therefore represent alternate outcomes of similar PCD programs.
When assessing how BAX could be activated in necroptosis induced by high doses of MNNG, we have focused on calpains.8 Ruling out the hypothesis of the direct activation of BAX by calpain cleavage, and keeping in mind that BCL-2 and BAX regulate MNNG-mediated PCD, we have examined the third subfamily of BCL-2 proteins: the BH3-only subgroup. More precisely, our attention was directed to BAD, BIM and BID, three BH3-only proteins that could be activated by calpains. In this sense, our data willingly demonstrate that BAD and BIM are dispensable, whereas BID is essential to MNNG-induced necroptosis. More precisely, our data reveal that the Ca2+-dependent calpain Cys proteases control BAX activation via a cleavage of BID into tBID at Gly70. Notably, a single mutation in this amino acid is sufficient to preclude AIF-mediated necroptosis. This is an important finding that provides a major target in this PCD program. Additionally, calpain inhibitors and calcium chelators are now evinced as attractive tools in the regulation of the necroptotic pathway.
BID appears to link PARP-1 and calpains activity to BAX activation and tAIF-release in MNNG-mediated necroptosis. This is a key issue because the presence of tAIF in the cytosol and further in the nucleus is essential to the generation of the DNA-degrading complex that manages necroptosis.14 These findings are in line with previous work on the relationships between BID and tAIF performed on purified mitochondria, on neuronal cell death induced by excitotoxic stimuli, oxidative stress, β-amyloid exposure, and on breast cancer cell death exposed to photodynamic therapy.13, 24, 25, 36, 37
As part of the molecular exploration of necroptosis, this work is indeed a first step in the analysis of the role of the BH3-only protein, BID. Ensuing questions arise: could BID favor AIF mitochondrial release by altering the mitochondrial morphology, as in other apoptotic systems?25 Are MTCH2/MIMP38 implicated in the mitochondrial recruitment of tBID in necroptosis? Future steps in the study of this BH3-only protein in necroptosis will unravel the mechanisms that control BID-mediated BAX activation and AIF-release, and will evaluate whether these mechanisms are similar in caspase-dependent and caspase-independent PCD.
Together with BID, two other BH3-only proteins have already been related to cell death programs with necrotic morphology: BMF and BNIP3.39, 40 Their existence brings new perspectives to the development of small molecules mimicking the activity of BH3-only proteins. Compounds such as Gossypol, ABT-263 or GX15-070 have been designed to overcome the overexpression of anti-apoptotic proteins in cancer cells. If these BH3-mimetics can also target necroptotic pathways, such drugs could find wider applications than initially expected. In this sense, the implication of necroptosis is described in brain ischemia, myocardial infarction, neurodegenerative diseases, head trauma, therapeutic killing of tumor cells, and so on.5, 6 Thus, by shedding new light on the mechanisms regulating necroptosis, our new findings pave the way for novel pharmacological strategies that could target aberrant PCD. Furthermore, our work introduces the concept that, as in classical apoptosis, the entire BCL-2 family of proteins is at the crossroads of the necroptotic pathways.
Materials and Methods
Cell culture and cell death induction and inhibition
MEFs were cultured in DMEM supplemented with 10% FCS, 2 mM L-glutamine, and 100 U/ml penicillin/streptomycin (Invitrogen, Carlsbad, CA, USA), and maintained at 37 °C in a 5% CO2 atmosphere. BAX−/− MEFs were provided by SJ Korsmeyer (Dana–Farber Cancer Institute, USA), BIM−/− by DC Huang (Walter and Eliza Hall Institute, Australia), BAD−/− by NN Danial (Harvard Medical School, USA), CAPN4−/− by PA Greer (Queen's University, Canada), and RIP1−/− by MA Kelliher (UMass Medical School, USA). BID−/−, BID−/− MEFs stably transfected with BID-wt or a non-phosphorylatable BID-S61A/S78A were previously described.26 BAX−/−, BIM−/−, BAD−/−, BID−/−, and RIP1−/− MEFs are derived from C57BL/6 mice and CAPN4−/− from 129SvJ mice. BAX−/−, BIM−/−, BAD−/−, and BID−/− are SV40 immortalized. RIP1−/− and CAPN4−/− MEFs were immortalized by a classical 3T3 dilution method. WT cells from each lineage were used as a control.
To induce death, cells were treated with MNNG (500 μM, ABCR GmbH, Karlsruhe, Germany) for 20 min. The treating medium was replaced after incubation by a fresh medium devoid of MNNG, and cells (80% confluence) were cultured at the indicated times. As a control of caspase-dependent apoptosis, cells were incubated 6 h with STS (1 μM) or etoposide (20 μM) or 9 h with TNF/CHX (50 ng/ml/1 μg/ml). In some experiments, cells were pre-incubated for 1 h with Q-VD.OPh (QVD, 10 μM, MP Biomedicals, Illkirch, France) or necrostatin-1 (10 μM) before treatment.
Vectors and lentiviral transduction
Murine BID-wt, BID-D59A, BID-G70A, and BID-Δ68-71 cDNAs tagged with a V5 epitope were cloned into the pLVX-IRES-Zs-Green lentiviral vector (Clontech, Ozyme, St. Quentin en Yvelines, France). Viruses were produced into 293T cells by CaCl2 transient transfection of the lentiviral constructs, and the packaging plasmids pMD2.G and psPAX-2 (Addgene plasmids 12 259 and 12 260, respectively). 48 h after transfection, lentiviral supernatants were harvested, clarified by filtration, and used immediately for BID−/− MEFs transduction with 4 μg/ml of polybrene. A total of 72 h after transduction, cells were diluted for immunoblot selection of individual clones. Clones with similar BID levels to WT BID MEFs were selected, expanded, and analyzed.
Flow cytometry
We used Annexin V-APC (0.1 μg/ml) for the assessment of PS exposure, PI (0.5 μg/ml) for cell viability analysis, and tetramethylrhodamine ethyl ester (20 nM) for ΔΨm quantification. Cell death was recorded in a FACSCanto II (BD Biosciences, Le Pont-De-Claix, France) in the total population (10 000 cells) and data were analyzed using FlowJo software (Tree Star, Ashland, OR, USA).
Determination of ATP content
Cells treated as indicated above were lysed and the total ATP content was assessed with a luciferin-luciferase kit. Luminescence was measured in a Berthold LB96V MicroLumat Plus. ATP content is expressed as relative to the number of cells analyzed in arbitrary units. A value of 100% refers to the ATP quantified in control cells.
Poly(ADP ribose) assessment
For immunoblotting detection, 1 × 106 cells were lysed in RIPA buffer containing 25 mM Tris-HCl (pH 6.0), 150 mM NaCl, 1% sodium deoxycholate, 0.1% SDS, and 1% NP-40. Samples were resolved on 5–8% SDS-polyacrylamide gels and transferred onto a PVDF membrane using a semi-dry method. Membrane blocking and antibody incubations were performed in PBS 0.1% Tween-20 plus 5% non-fat dry milk. Membranes were probed with an anti-PAR antibody (Clone 10 H, Alexis, Illkirch, France), immunoreactive proteins were detected by an HRP-conjugated anti-mouse antibody, and revealed by enhanced chemiluminescence (ECL, Pierce, Thermo Fisher Scientific, Brebières, France).
Alternatively, cells seeded on coverslips were fixed in ice-cold methanol for 15 min, permeabilized with 0.1% Triton X-100, pre-incubated in 10% goat serum for 30 min, incubated with the anti-PAR antibody, and detected by anti-rabbit IgG conjugated with Alexa Fluor 488 (Invitrogen). Before assessment, cells were co-stained with Hoechst 33 342 and mounted with FluorSave (Calbiochem, Merck, Darmstadt, Germany). Green and blue fluorescences were recorded in a confocal microscope (LSM-510 Meta, Carl Zeiss France, Le Pecq, France). Quantification was performed on 150 cells for each data point.
Calpain activity
Calpain activity present in 30 μg of total-cell lysates was determined by cleavage of the fluorescent substrate N-succinyl-LLVY-AMC (Calbiochem) and expressed as the difference between calcium-dependent and calcium-independent fluorescence. Calcium-dependent fluorescence was measured after 30 min incubation at 37°C in buffer containing 63 mM imidazole-HCl (pH 7.3), 10 mM β-mercaptoethanol and 5 mM CaCl2. Calcium-independent fluorescence was measured under the same conditions using buffer without CaCl2 and containing 1 mM EDTA and 10 mM EGTA. Fluorescence was recorded in a Fluoroskan Ascent Fluorimeter (Labsystems, Eragny-Parc, France).
Calpain activity in live cells was detected using the cell-permeable calpain substrate Boc-Leu-Met-CMAC (Invitrogen). Cells spread on coverslides were incubated for 30 min with 50 μM of calpain substrate in DMEM culture medium. Cells were then treated or not with MNNG and observed in a Nikon Eclipse TE2000-U microscope (Nikon France, Champigny-Sur-Marne, France). Quantification was performed on 100 cells for each data point.
BAX activation and redistribution
For flow cytometry or immunofluorescence analysis, cells were fixed in 1% paraformaldehyde (PFA), permeabilized with 0.1% saponin for 10 min, incubated for 1 h with an antibody designed against the active form of BAX (clone 6A7, BD Biosciences, ref. #556467), and detected by an anti-mouse IgG conjugated with Alexa Fluor 488 or 647. Cells were either analyzed by cytofluorometry or stained with Hoechst 33 342 and incubated with Mitotracker Red before fixation for immunofluorescence assessment. Images were acquired with a confocal microscope as above. Quantification was performed on 100 cells for each data point.
3′-OH DNA breaks (TUNEL) detection
Detection of blunt double-stranded fragments carrying a 5′-phosphate and 3′-hydroxyl group was carried out by fixing cells in freshly prepared 1% PFA for 30 min at 4 °C and permeabilized with 0.1% Triton X-100 and 0.1% sodium citrate for 10 min at 4 °C. After washing in PBS containing 1% BSA, cells were incubated for 1 h at 37 °C with 50 μl of a reaction mixture containing 0.025 nmol of Fluorescein-12-dUTP (Roche, Meylan, France) or CF 640R-dUTP (Biotium, Hayward, CA, USA), 0.25 nmol of dATP, 2.5 mm CoCl2, 80 units of recombinant terminal deoxynucleotidyl transferase (TdT) and TdT reaction buffer from Roche. After being washed in PBS, cells were analyzed by flow cytometry.
BID modeling
BID structure (pdb 1DDB) was modeled in SwissPDBViewer (Swiss Institute of Bioinformatics, Lausanne, Switzerland) and the resulting model was edited with Pymol (Delano Scientific, San Carlos, CA, USA), as previously described.14
Single cell gel electrophoresis (COMET)
Neutral comet assay was performed with a kit from Trevigen following the manufacturer's instructions. Single-cell images were captured and analyzed using a Nikon Eclipse TE2000-U microscope as indicated above. Quantification was performed on 100 cells for each data point.
MTT cell viability
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, 0.5 mg/ml final concentration) was added to the culture medium of cells growing in 96-well dishes (6000 cells/well). After incubating the dishes at 37 °C for 90 min, the assay was stopped by adding 100 μl DMSO. Formazan salts were allowed to dissolve in DMSO shaking them gently for 10 min at room temperature (RT), and the assays were quantified by means of a Multiskan plate reader (ThermoLabsystems, Cergy Pontoise, France). Final values were the result of subtracting 630 nm from 590 nm readings.
Cell cycle analysis and BrdU staining
Cells were harvested, washed once in PBS, fixed in ice-cold ethanol, and kept at −20 °C. DNA contents were assessed by using PI (20 μg/ml) after 20 min incubation with RNAse A (160 μg/ml).
To determine DNA synthesis, cells were pulse-labeled with 30 μM BrdU for 30 min. Cells were fixed for at least 16 h and membranes were digested with 0.05% pepsin in 30 mM HCl at 37 °C for 20 min. To allow the access of anti-BrdU antibody to the incorporated BrdU, DNA denaturation by 2N HCl was performed for 20 min at RT. Following centrifugation, acid was neutralized by adding 0.1 M sodium borate for 4 min. Cells were then centrifuged, washed, and FITC-conjugated anti-BrdU antibody (BD Biociences) was added for 45 min at RT. The incubation buffer used was 20 mM HEPES, 0.5% FCS, 0.5% Tween-20. DNA was stained with PI before flow cytometer analysis.
Protein extraction, cell fractionation, immunoblotting
For whole-protein extracts, 1 × 106 cells were lysed in buffer containing 20 mM Tris-HCl (pH 7.6), 150 mM NaCl, and 1% Triton X-100. For cytosolic purification, 2 × 106 cells were resuspended in buffer containing 220 mM mannitol, 70 mM sucrose, 50 mM Hepes-KOH (pH 7.2), 10 mM KCl, 5 mM EGTA, 2 mM MgCl2, and 0.025% digitonin, and kept on ice for 5 min. Lysed cells were centrifuged (16 000 × g, 5 min, 4 °C) and the supernatant was retained as cytosolic fraction. Mitochondrial fractions were obtained using the Mitochondria Isolation kit from Pierce following the manufacturer's instructions. Protein concentration was determined using the BioRad protein assay. Equal amounts of total proteins (40–100 μg) were loaded on 4–12% NuPAGE or linear SDS-PAGE gels and transferred onto a PVDF membrane. Membrane blocking and antibody incubations were performed in PBS 0.1% Tween-20 plus 5% non-fat dry milk. The primary antibodies used were: BAX (specific for BAX p18 detection, BD Biosciences), AIF, BID/tBID (clone 14F2 Novus Biologicals, Cambridge, UK or AF860 R&D Systems, Lille, France), phospho BIDS78, phospho BIDS61 (Bethyl, Montgomery, TX, USA), pan-ERK (BD Biosciences), Cox IV subunit VIb (Invitrogen), V5 (clone V5-10), or β-actin (clone AC-15). Immunoreactive proteins were detected using HRP-conjugated secondary antibodies and revealed by the ECL system. In some experiments, equal loading was confirmed by staining the membrane with naphtol blue. Immunoblot images were acquired in a Bio-Imaging System MF-ChemiBis 4.2 (FSVT, Courbevoie, France) or in a Kodax X-OMAT 1000 processor (Kodak, Chalon-Sur-Saone, France).
Statistics
Statistical analysis was carried out using the student's t-test.
Chemicals and reagents were purchased from Sigma-Aldrich except where otherwise noted.
Abbreviations
- AIF:
-
Apoptosis-inducing factor
- BAD:
-
Bcl-2-associated death promoter
- BAX:
-
Bcl-2–associated X protein
- BAK:
-
Bcl-2 homologous antagonist/killer
- BCL-XL:
-
B-cell lymphoma-extra large
- BCL-2:
-
B-cell lymphoma 2
- BCL-W:
-
Bcl-2-like protein 2
- BID:
-
BH3 interacting domain death agonist
- BIM:
-
Bcl-2 interacting mediator of cell death
- BNIP3:
-
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
- BMF:
-
Bcl-2-modifying factor
- CHX:
-
Cycloheximide
- DMEM:
-
Dulbecco's Modified Eagle's Medium
- DMSO:
-
Dimethyl sulfoxide
- FCS:
-
Fetal calf serum
- JNK1:
-
c-Jun NH2-terminal kinase 1
- MCL-1:
-
Myeloid cell leukemia sequence 1
- MTCH2/MIMP:
-
Mitochondrial carrier homolog 2
- Q-VD.OPh:
-
Gln-Val-Asp(non-Omethylated)-Oph
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Acknowledgements
We are grateful to Drs. SJ Korsmeyer, NN Danial, DCS Huang, PA Greer and MA Kelliher for immortalized MEFs, RS Moubarak for preliminary data, and M Segade for proofreading. This work was supported by Agence Nationale de la Recherche (ANR; contract ANR-09-BLAN-0247), Association pour la Recherche sur le Cancer (ARC; contracts 5104 and 7987), Ligue Contre le Cancer, and Fondation de France (to SA Susin). L Cabon received a PhD fellowship from École Normale Supérieure de Cachan (ENS-Cachan).
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Cabon, L., Galán-Malo, P., Bouharrour, A. et al. BID regulates AIF-mediated caspase-independent necroptosis by promoting BAX activation. Cell Death Differ 19, 245–256 (2012). https://doi.org/10.1038/cdd.2011.91
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DOI: https://doi.org/10.1038/cdd.2011.91
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