Abstract
The human lymphocyte toxins granzyme B (hGrzB) and perforin cooperatively induce apoptosis of virus-infected or transformed cells: perforin pores enable entry of the serine protease hGrzB into the cytosol, where it processes Bid to selectively activate the intrinsic apoptosis pathway. Truncated Bid (tBid) induces Bax/Bak-dependent mitochondrial outer membrane permeability and the release of cytochrome c and Smac/Diablo. To identify cellular proteins that regulate perforin/hGrzB-mediated Bid cleavage and subsequent apoptosis, we performed a gene-knockdown (KD) screen using a lentiviral pool of short hairpin RNAs embedded within a miR30 backbone (shRNAmiR). We transduced HeLa cells with a lentiviral pool expressing shRNAmiRs that target 1213 genes known to be involved in cell death signaling and selected cells with acquired resistance to perforin/hGrzB-mediated apoptosis. Twenty-two shRNAmiRs were identified in the positive-selection screen including two, PCAF and ADA3, whose gene products are known to reside in the same epigenetic regulatory complexes. Small interfering (si)RNA-mediated gene-KD of PCAF or ADA3 also conferred resistance to perforin/hGrzB-mediated apoptosis providing independent validation of the screen results. Mechanistically, PCAF and ADA3 exerted their pro-apoptotic effect upstream of mitochondrial membrane permeabilization, as indicated by reduced cytochrome c release in PCAF-KD cells exposed to perforin/hGrzB. While overall levels of Bid were unaltered, perforin/hGrzB-mediated cleavage of Bid was reduced in PCAF-KD or ADA3-KD cells. We discovered that PCAF-KD or ADA3-KD resulted in reduced expression of PACS2, a protein implicated in Bid trafficking to mitochondria and importantly, targeted PACS2-KD phenocopied the effect of PCAF-KD or ADA3-KD. We conclude that PCAF and ADA3 regulate Bid processing via PACS2, to modulate the mitochondrial cell death pathway in response to hGrzB.
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Main
Human GrzB (hGrzB) is a pro-apoptotic protease that induces target cell death by activating the mitochondrial (intrinsic) cell death pathway in a perforin-dependent manner.1, 2, 3, 4, 5 Like caspases, hGrzB processes substrate proteins after key aspartate residues (‘asp-ase activity’), but with substantially different fine substrate specificity. After entry into the target cell cytoplasm, a key early step is cleavage of the BH3 domain-only protein Bid at a dedicated hGrzB site (Asp-75), distinct from the caspase site (Asp60).6, 7 Translocation of truncated Bid (tBid) to mitochondria results in destabilization and permeabilization of mitochondrial outer membrane (MOMP) and release of cytochrome c and Smac/Diablo from the intermembrane space. Caspase activation through the mitochondrial pathway is a hallmark of hGrzB: although the granzyme can directly initiate the processing of pro-caspase-3 (and several other effector caspases), minimal caspase activity results as further auto-processing of pro-caspase-3 occurs only when Smac/Diablo displaces inhibitor of apoptosis proteins (IAP's) from the partly processed pro-caspases. Smac/Diablo release also induces apoptosome formation that activates caspase-9. Overexpression of Bcl-2 (Bcl-2-overexpression) or its viral orthologue BHRF1 in human cells very effectively blocks hGrzB, as they inhibit MOMP, resulting in cytochrome c and Smac/Diablo retention within mitochondria.8, 9 By contrast, subtle species differences in proteolytic specificity dictate that mouse GrzB preferentially activate pro-caspases directly, rather than cleaving Bid.1, 10 Mouse GrzB is therefore less prone to inhibition by Bcl-2 and like inhibitors.1
The fact that Bcl-2-overexpression confers both short-term resistance to perforin/hGrzB and enables long term clonogenic survival indicates that Bid-mediated MOMP is the dominant cell death signaling pathway activated by hGrzB. This is also borne out kinetically: Bid cleavage occurs within 60 s of hGrzB accessing the cytosol and MOMP follows in as little as 3–5 min.11 Nonetheless, a variety of studies have shown that hGrzB can also cleave other intracellular proteins with variable efficiency, and it is likely that some (or perhaps many) may have physiological relevance, including filamin, gelsolin and ROCKII.12, 13, 14 Many viruses express Bcl-2-like proteins, and we have shown that the EBV orthologue BHRF1 is a potent blocker of hGrzB-mediated cell death. Despite this, intact cytotoxic lymphocytes (CTLs) (as distinct from purified recombinant reagents) are able to circumvent a Bcl-2-like block,8 indicating that alternative pathways to cell death must exist.
Given the dominant role of hGrzB in human CTLs or natural killer (NK) cell -mediated cell death and the plethora of putative substrates, further understanding the mechanisms involved in hGrzB-induced death is of great importance. We chose to investigate this by performing a gain-of-representation gene-knockdown screen that examined the impact of knockdown of ∼1213 genes reported to have a role in programmed cell death. Amongst 22 genes reproducibly identified in the screen, we identified epigenetic regulators p300/CBP associated protein (PCAF) and transcriptional adaptor 3 (ADA3), known to interact within the same macromolecular complex, as regulators of perforin/hGrzB-mediated Bid cleavage and intrinsic apoptosis. Further mechanistic insight was provided by our discovery that knockdown of a potential downstream target gene of PCAF and ADA3, phosphofurin acidic-cluster sorting-protein 2 (PACS2), also resulted in reduced Bid cleavage and apoptosis following treatment with perforin/hGrzB. Our studies therefore identified a novel integrated molecular pathway that regulates a cell’s susceptibility to perforin/hGrzB.
Results
A functional genomics screen identifies genes that regulate perforin/hGrzB-mediated apoptosis
A library of ∼4000 shRNAmiRs directed against 1213 genes (Supplementary Table 1) previously involved in cell death were pooled, packaged into lentivirus and used to transduce HeLa cervical cancer cells (HeLa) (Supplementary Figure 1). Given significant species differences in substrate specificity, all of our experiments (both in the initial screen and all subsequent studies) utilized only human target cells and hGrzB. Stably transduced HeLa were selected by growth in medium containing puromycin. A low multiplicity of viral infection that favored single lentivirus integration per cell was used, resulting in ∼20% of the starting population acquiring puromycin resistance and expressing the GFP reporter protein (data not shown). After recovering for 48 h and resuming proliferation in selective medium, cells were exposed to a normally lethal concentration of recombinant hGrzB (60 nM), combined with a sublytic quantity of recombinant perforin (1 nM). This concentration of hGrzB was chosen as it is sufficient to induce >80% specific 51Cr release from non-silencing (NS)-shRNAmir transduced control cells in a standard 4-hour assay, or render >80% of cells permeable to the vital dye Alamar blue (AB) 24 h after treatment (data not shown). By contrast, the same concentration of perforin applied alone produced <10% specific 51Cr release and had no significant effect on long-term viability (data not shown). To select for cells that had acquired resistance to perforin/hGrzB, pools of transduced HeLa were expanded and exposed to three further rounds of selection (Supplementary Figure 1), to produce cell populations significantly resistant to hGrzB-induced apoptosis (Hit 4), compared with NS controls (P<0.05, Supplementary Figure 2a).
To identify specific shRNAmiRs that conferred resistance to perforin/hGrzB, the pool of resistant cells was cloned and DNA was extracted for sequencing. We identified 25 shRNAmiRs targeting 22 candidate genes. Two different shRNAmirs targeting GTF2H3, MAPK8 and TGFBR1 were identified in the original screen (Supplementary Figure 2b). To validate the candidate genes, HeLa expressing the same individual shRNAmiRs as identified in the screen, were exposed to perforin/hGrzB (60 nM) (Supplementary Figure 2c). As Bcl-2-overexpression powerfully suppresses hGrzB-mediated death,8 Bcl-2-overexpressing HeLa were used as controls (Supplementary Figure 2c). When knocked down, five gene candidates displayed consistent resistance to perforin/hGrzB (PCAF, ADA3, YWHAQ, PKR and WDR33). A further validation step was performed using shRNAmiRs directed to different regions of the gene sequences (Supplementary Figure 2di). The results consistently showed that KD of PCAF, ADA3, YWHAQ, PKR and WDR33 provided significant resistance to perforin/hGrzB-mediated apoptosis (P<0.001, Supplementary Figure 2di). Furthermore, protection conferred by the depletion of these five candidates was comparable with Bcl-2 overexpression, (Supplementary Figure 2di). To confirm our findings, an independent technology, siRNA-mediated gene KD was used. Once again, depletion of PCAF, ADA3, YWHAQ, PKR and WDR33 provided reproducible and statistically significant suppression of apoptosis when compared with siRNA for a non-targeting (NT) region or GAPDH-KD (P<0.001, Supplementary Figure 2dii).
Reduced expression of PCAF or ADA3 confers resistance to perforin/hGrzB
To further test the robustness of our findings, four additional shRNAs targeting different regions of the PCAF mRNA and the ADA3 shRNA, were examined. PCAF shRNAs PCAF, −88, −89, −90 and −91 reduced steady-state PCAF protein, albeit at varying levels depending on the shRNA, and all significantly reduced mRNA levels by ∼75% compared with NS (P<0.001, Figure 1ai). ADA3 mRNA and protein were also significantly reduced by the ADA3 shRNA (P<0.001, Figure 1aii). All five PCAF shRNAs and the ADA3 shRNA, protected HeLa from perforin/hGrzB in 4 h 51Cr release assays (Figures 1bi–iii), while long-term cell viability (AB exclusion at 24 h, and continued proliferation) was also observed to a similar level as Bcl-2 overexpression (Figures 1ci and ii). Similar results were also achieved with individual siRNAs targeting PCAF or ADA3 (Supplementary Figure 3b and c).
PCAF is a transcriptional co-factor that acetylates histone and non-histone proteins,15, 16 while ADA3 is an important transcriptional adaptor protein that binds to PCAF in both the hSAGA and ATAC complexes.17 The fact that PCAF and ADA3 had been independently identified in our screen strongly suggested a novel epigenetic/transcriptional mechanism was responsible for regulating sensitivity to perforin/hGrzB. If PCAF and ADA3 operate in the same signaling pathways,17 we predicted that reducing the levels of both would have no greater effect than suppressing either alone. We found it difficult to maintain cell viability when both genes were suppressed at similar levels as achieved individually (data not shown). Ultimately, we produced cells in which PCAF expression was reduced by ∼50% and ADA3 by ∼25% (Figure 2ai-ii), but protection against perforin/hGrzB was not further enhanced (Figure 2b).
How does suppressing PCAF levels endow protection against hGrzB?
We sought to determine whether PCAF was exerting its effect on perforin/hGrzB-mediated death upstream or downstream of MOMP.3, 6, 18, 19 The release of cytochrome c was markedly reduced in cells with downregulated PCAF, whereas the broad-spectrum caspase inhibitor Q-VD-OPh (QVD), which exerts its effect downstream of MOMP, did not enhance protection (Figure 3). This indicated that protection was occurring at or proximal to the mitochondria. We examined steady-state mRNA and protein levels for Bid and mRNA levels for Bcl-2, but neither was significantly changed (Supplementary Figures 4a and bi), nor were mRNA levels of other Bcl-2 family members (Mcl-1, Bcl-xl) (Supplementary Figures 4bii–iv). A significant increase in Bcl-w expression was also observed but was insufficient to impart protection. In other studies, the pro-survival effect of Bcl-w has been shown to be restricted to sperm and epithelial cells derived from the gut.20, 21 Extending this ‘candidate’ approach, we also found normal levels of mRNA and protein for Smac/Diablo, Bax and cytochrome c (data not shown). We next examined whether PCAF- or ADA3-KD conferred protection against other death stimuli that operate through the mitochondrial pathway. Ligation of the death receptor TRAIL initiates apoptosis through the apical procaspase-8 (or occasionally, -10) and is negatively regulated by Bcl-2 in a cell line-specific manner.22, 23 HeLa with downregulated PCAF or ADA3 were not protected against TRAIL and continued to process pro-caspases-8 and -3 normally, whereas Bcl-2 overexpression strongly suppressed cell death (Figures 4ai and ii and bi and ii and Supplementary Figure 5d). Results were similar when we exposed the same cells to ultraviolet radiation (UV), which also activates the mitochondrial cell death pathway24 (Figure 4ci–ii and Supplementary Figure 5e).
Reduced Bid processing in HeLa cells depleted of PCAF or ADA3
Due to the significant reduction in cytochrome c release in PCAF-depleted HeLa (Figure 3), we investigated the potential involvement of Bid in its direct processing as hGrzB is a pivotal initiating step leading to MOMP1, 4, 6 and the removal of Bid has been shown to mediate resistance to perforin/hGrzB.6, 25 We generated Bid-depleted HeLa (Bid-KD) (Supplementary Figure 5a) and found they displayed similar levels of resistance to perforin/hGrzB-mediated cell death as PCAF-KD or ADA3-KD cells, compared with NS controls (Supplementary Figure 5c, P<0.001). Bid-KD also showed incomplete caspase-3 (CSP3) processing from p20 to p17 following exposure to perforin/hGrzB and, similar to PCAF-KD cells, decreased cytochrome c release compared with NS (Supplementary Figures 5a and b). These similarities between Bid-KD and PCAF- or ADA3-KD cells upon perforin/hGrzB treatment suggested that Bid processing may be affected in PCAF- or ADA3-KD cells. Although we have shown Bid mRNA and protein levels were not reduced as a result of PCAF or ADA3 depletion (Supplementary Figure 4a), we assessed Bid processing in these cells. We found a significant reduction of Bid processing when either PCAF or ADA3 was depleted (Figures 5a–c), even when high concentrations of hGrzB (100 nM) were used (Figure 5c). This was also seen in HCT116 colon cancer cells when depleted of PCAF or ADA3, particularly in PCAF-KD cells (Supplementary Figure 7b). The slightly slower-migrating caspase-cleaved tBid was also observed, and was lost with the addition of QVD, with minimal effect on the hGrzB-cleaved form (Figures 5a and b). Reduced Bid cleavage was also evident in cells depleted of both PCAF and ADA3 (Figure 5d).
It was possible that ADA3 and/or PCAF might transcriptionally regulate Bid processing by affecting expression of the cognate hGrzB inhibitor SERPIN-B9 (formerly known as PI9).26 However, SERPIN-B9 mRNA and protein levels were not altered in PCAF- or ADA3-depleted cells (Supplementary Figure 6a). To test for an alternate direct hGrzB inhibitor (although none has ever been described), purified recombinant hGrzB was incubated with graded amounts of lysate from NS, PCAF- or ADA3-depleted HeLa. The inhibitory effect of PCAF- or ADA3-depleted HeLa cell lysate on hGrzB’s proteolytic activity was similar to control (Supplementary Figures 6bi and ii and data not shown). In addition, we found no variation in perforin-dependent hGrzB internalization in NS, PCAF or ADA3-depleted cells (Supplementary Figure 6c).
Another possibility was PCAF or ADA3 directly catalyzed hGrzB’s cleavage of Bid, by directly associating with Bid. We considered this unlikely, as Bid is localized in the cytosol, whereas PCAF and ADA3 are typically nuclear proteins. Nonetheless, PCAF may shuttle between the nucleus and cytosol under certain circumstances.27 We formally excluded this possibility in two ways: first, we used co-immunoprecipitation (IP) to show that, as expected, ADA3 and PCAF form a complex (Figure 6a and data not shown) with one another, but this did not include Bid (Figures 6bi and ii). Reciprocal immunoprecipitations using antibodies specific for Bid showed Bid did not co-precipitate PCAF (Figure 6biii) or ADA3 (data not shown). Second, we examined the subcellular localization of ADA3 and PCAF, both constitutively and following exposure to lethal concentrations of perforin/hGrzB. Immunoblot analysis demonstrated that PCAF and ADA3 were located in the nuclear fraction of lysed HeLa while Bid remained in the cytosol (Figures 6ci and ii). PCAF remained nuclear, both constitutively and when the cells were incubated with perforin/hGrzB (Figure 6ciii). These findings were also confirmed for PCAF by immunofluorescence staining (Figures 6di and ii).
Is Bid post-translationally modified in the presence or absence of PCAF or ADA3?
We considered whether gene regulatory activities of PCAF or ADA3 might affect Bid processing by influencing post-translational modifications of full-length Bid, or through modified clearance of its pro-apoptotic cleavage products. For example, phosphorylation of mouse Bid on Ser61, Ser64 or Thr59 flanking the caspase cleavage site (Asp60) can inhibit Bid processing by caspase-8 or -3.7, 28 Furthermore, Ser-78 adjacent to the dedicated hGrzB cleavage site can be transiently phosphorylated in response to DNA double-strand breaks.29 However, we detected no change in Ser78 phosphorylation in PCAF-depleted cells in either the absence or presence of perforin/hGrzB (Figure 7a, upper 2 panels); by contrast, Etoposide-treated HeLa displayed pronounced Ser78 phosphorylation. Phosphorylation of Ser65 also did not change with perforin/hGrzB exposure (Figure 7a, middle panel) and cleavage of Bid persisted (Figure 7a, lower panels). Furthermore, inhibiting proteasomal degradation of tBid with Bortezomib did not affect reduced tBid levels in PCAF-depleted cells (Figure 7b), ruling out accelerated tBid degradation as the mechanism of enhanced protection.
PACF or ADA3 depletion results in reduced PACS2 expression and PACS2 depletion phenocopies reduced Bid processing
Transport of Bid to the mitochondria is an important step leading to oligomerization of Bax/Bak to initiate MOMP,3, 30 and this in turn requires PACS2, which binds unprocessed Bid.31 PACS2-Bid binding is enhanced following Fas ligation, and conversely, depletion of PACS2 leads to decreased Bid processing.31 Given that PCAF and ADA3 are transcriptional co-activators,15, 16, 17 we determined whether depletion of PCAF or ADA3 affected PACS2 expression. We found a significant reduction (P<0.01) in PACS2 expression in PCAF- and ADA3-depleted HeLa and HCT116 cells (Figure 8a and Supplementary Figure 7c). Next we used an shRNA directed against PACS2 to determine whether a reduction in PACS2 expression (Figure 8b) was sufficient to induce resistance to perforin/hGrzB-mediated cell death. PACS2 depletion led to significant protection against perforin/hGrzB (P<0.01), as demonstrated by reduced 51Cr release (Figure 8ci). Furthermore, PACS2-KD also reduced Bid processing following perforin/hGrzB exposure (Figure 8cii).
Discussion
Immune-mediated death of infected or transformed human cells follows exocytic release of the serine protease GrzB that rapidly accesses the target cell cytosol via perforin pores.11 The proteolytic processing of Bid to active tBid is a pivotal step initiating intrinsic apoptotic pathway, triggering MOMP and subsequent release of cytochrome c and Smac/Diablo.3, 4, 5, 6, 18 Further elucidating perforin/hGrzB-mediated cell death was warranted to aid our understanding of how diseased cells evade death. We performed a knockdown screen targeting 1213 genes associated with cell death signaling and selected candidates whose loss suppressed perforin/hGrzB-mediated apoptosis. Among 22 genes identified from the screen, we found that loss of either PCAF or ADA3, two proteins that associate within the same epigenetic regulating complexes,17 resulted in cell survival. The sustained resistance to cell death resulting from PCAF or ADA3 deficiency mapped upstream of mitochondria, as indicated by reduced cytochrome c release in response to perforin/hGrzB; in turn, this was associated with reduced cleavage/processing of Bid by hGrzB.
PCAF and ADA3 associate in the same signaling complexes to epigenetically regulate gene transcription.17 We systematically ruled out several resistance mechanisms that might have been recruited following PCAF- or ADA3-depletion: we found no evidence that a hGrzB inhibitor was induced, and specifically ruled out the irreversible, cognate serpin inhibitor SERPIN-B9. The expression levels of anti-apoptotic Bcl-2 family proteins was unaltered, and there was no evidence that either PCAF or ADA3, reported to shuttle to the cytoplasm under certain circumstances, interact directly with Bid to facilitate its cleavage; rather, as reported previously PCAF and ADA3 co-precipitated with each other (but not with Bid) and remained localized in nuclei of both healthy and dying cells. Suppression of cell death was observed to be hGrzB specific, as apoptosis mediated by TRAIL or UV, which rely on caspase processing23, 24 was not affected. HeLa are ‘Type II’ cells that use the intrinsic pathway for TRAIL-mediated death, as shown by the fact that Bcl-2 overexpression results in protection.32, 33 It is not clear why PCAF/ADA3 depletion failed to protect against TRAIL (even at low concentrations); however, it appears activated caspase-8 remains capable of directly activating caspase-3 when Bid processing is reduced. We sought to understand the mechanism(s) of reduced Bid processing in PCAF- or ADA3-depleted cells, but identified no change in its phosphorylation status, reported to influence its processing by caspases.28, 29 Of particular importance, we confirmed that Ser-78, located close to the hGrzB Bid-cleavage site (Asp-75), was phosphorylated in response to the DNA-damaging agent etoposide29 but not after perforin/hGrzB exposure. PCAF has been shown to have ubiquitinase activity,34 but blocking the proteasome with Bortezomib did not affect tBid degradation.
Trafficking of dephosphorylated Bid to mitochondria is enhanced following extrinsic cell death activation with anti-Fas antibody31 and is regulated by the transporter PACS2. Indeed, PACS2-KD reduced Bid processing following Fas receptor cross-linking.31 We showed for the first time that PACS2 expression was reduced in PCAF- or ADA3-depleted cells, suggesting that PACS2 is a downstream target of PCAF and/or ADA3.17 Furthermore, independent knockdown of PACS2, conferred resistance to perforin/hGrzB and phenocopied reduced processing of Bid observed in PCAF- or ADA3-depleted cells. Our studies therefore identified a novel integrated molecular pathway regulating the target cell’s response to perforin/hGrzB (Figure 8d).
Our findings provide important new information on cellular processes that regulate responses to primary apoptotic effector proteins of cytotoxic lymphocytes. Reinforcing the dominance of the hGrzB signaling pathway, we recently showed pro-apoptotic granzymes act in a hierarchical fashion, recruiting diverse cell death pathways that all rely on access to the cell cytosol via perforin pores. Thus, in the absence of GrzB, cell death phenotype reverts to a slower, non-apoptotic cell death pathway (‘athetosis’), in which GrzA targets the actin cytoskeleton.35 This diversity and lack of reliance on a single death pathway are important for understanding how mammalian immune systems have stayed ahead in the evolutionary ‘arms race’ against intracellular pathogens. The literature records several ways in which hGrzB pathway may be blocked by viruses, including direct inhibition in vitro of GrzB by overexpressed viral serpins,36 or by the capsid proteins of human adenovirus V,37 or by elaboration of Bcl-2-like inhibitors such as Epstein-Barr virus BHRF1 to block signaling at the mitochondrion.9 In human cancer, the cognate hGrzB inhibitor SERPIN-B9 may be ectopically expressed to blunt CTL-mediated tumor cell attack.38 Our identification of an epigenetic mechanism through which sensitivity to hGrzB can be reset to render a target cell more or less sensitive to Bid-mediated MOMP has potential therapeutic implications in cancer. We recently showed that overexpression of Bcl-2 and like inhibitors in tumor cells can potentially be overcome by combining hGrzB and a BH3-mimetic agent such as ABT-737 and that the hGrzB death signal is remarkably long lived.39 The well-described capacity of various human cancers to downregulate PCAF or ADA3 levels40, 41, 42, 43 would be expected to blunt combination therapies such as this, by repressing the latent pro-apoptotic signal provided by tBid.
The involvement of PCAF and/or ADA3 in disease in normal development and a number of pathologies is well described. ADA3-null mice die in utero,44 probably due to a defect in cell cycle progression. ADA3 can upregulate the p53 tumor suppressor, while both ADA3 and p53 can be deregulated via the human papillomavirus oncoprotein E6, with implications in many cancers particularly cervical.43 ADA3 can bind to estrogen promoters to recruit histone acetyl transferases including PCAF to enhance receptor expression, consequential in estrogen-dependent tumor cell growth.45 Finally, ADA3 can regulate beta-catenin, a transcriptional activator of the Wingless-Int signaling pathway,46 de-regulation of which is implicated in various malignancies including acute myeloid leukemia.47 In contrast to ADA3, PCAF-null mice develop normally,48 but PCAF is implicated in a number of disease states including arteriosclerosis (including aneurysm formation) and Alzheimer’s disease.49, 50, 51 A specific PCAF gene polymorphism has also been linked to hepatocellular carcinoma.52 Malignancies associated with BRCA2 gene mutation may be linked to deregulated PCAF recruitment to target genes, leading to disordered cell division and aneuploidy.53 Finally, downregulation of PCAF has been observed in esophageal squamous cell carcinoma, ovarian and colorectal cancer.40, 41, 42 Exactly how reduced expression and/or function of PCAF and ADA3 might lead to human diseases including cancer remains unclear. Small molecule inhibitors of PCAF have been reported; however, further studies on their specificity and role in disease are warranted52, 54 as is a further exploration of the potential impact of PCAF/ADA3 on immune-based cancer therapies.
Materials and Methods
Cell culture and reagents
HeLa cells were maintained as previously described.55 HCT116 cells were maintained in RPMI (Gibco Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum. For Bcl-2-overexpression, cells were transfected and selected as previously described.18 For Bid-KD cells, the p-Retro-Super vector containing shRNA for Bid was a gift from Professor J Borst, Netherlands Cancer Institute.56 For non-silencing (NS), ADA3-KD and PCAF-KD cells, the pGIPZ vector containing the shRNAmir targeting NS, ADA3 or PCAF were obtained from Thermo-Fisher Scientific (Franklin, MA, USA). Cells expressing shRNA were selected with puromycin (2 ug/ml, Sigma-Aldrich, St. Louis, MO, USA) for 48 h and maintained in culture with 1ug/ml puromycin for stable cell line continuity. For the shRNA screen a library of ∼4000 shRNAmiRs directed against 1213 genes associated with cell death was obtained from Thermo-Fisher Scientific. Embedding shRNAs in an endogenous miR30 microRNA backbone was a major advance over previous shRNA screening vectors as the miR30 structure significantly enhances the shRNA efficiency through increased Drosha and Dicer processing of the expressed hairpins.57 The library used in this screen was at the time the most sophisticated resource available for high throughput shRNA screening. Recombinant hGrzB was produced in Pichia pastoris58 and recombinant mouse perforin was produced in a baculovirus expression system.55 For recombinant hGrzB, 20–60 nM was considered low–middle potency. Caspases were blocked with Q-VD-OPh (10 uM, Calbiochem, La Jolla, CA, USA). Human-TRAIL ligand was from Peprotech (Rocky Hill, NJ, USA). Cells were exposed to UV-light Joules/centimeter2 (J/cm2) using an Ultraviolet Crosslinker (CL1000, UVP, Upland, CA, USA). Cells were treated with 100 uM Bortezomib (Millennium Pharmaceuticals, Cambridge, MA, USA) for 1 h or with 100 uM Etoposide (Pfizer, New York, NY, USA), for 20 min.
Transfections and transductions
For retrovirus production, HEK293T cells were transfected with p-Retro-Super shRNA and pLMP using Fugene-6 according to the manufacturer's instructions (Promega, Madison, WI, USA). For lentivirus production the HEK293T cells were transfected with pGIPZ plasmids (Sequence and Catalog Reference: non-silencing, VGH5518; ADA3, 5′-CCAAGATCCAGGAATATGA-3′, V2LHS_253631; PCAF, 5′-CAACTAATATACAACCATA-3′, V2LMM_189282; PCAF-88, 5′-GTACTACGTGTCTAAGAAA-3′, V2LHS_53088; PCAF-89, 5′-CAAACAAGTTTATTTCTAT-3′, V2LHS_53089; PCAF-90, 5′-GCCCGAATCGCCGTGAAGA-3′, V2LHS_53090; PCAF-91, 5′-CAAGTTTATTTCTATCTAT-3′ V2LHS_53091; PACS2, 5′-CAGAGATACTGCAACTGCA-3′, V2LHS_91559) and LentiX-HT packaging mix (Clontech, Mountain View, CA, USA) using the polyethylenimine (PEI) transfection method, PEI was purchased from PolySciences (Warrington, PA, USA). Forty-eight hours after the transfection, media containing virus was collected and cells were transduced, cultured for 16 h and media was refreshed. Successfully transduced cells were selected with puromycin (2 ug/ml) for 48 h. For siRNA transfection of cells, the Thermo-Fisher Scientific reverse transfection method was used to transiently transfect cells with siRNA-SMARTPOOL (Thermo-Fisher Scientific) (catalog reference: non-targeting (NT), D-001810; PCAF, L-005055; ADA3, L-017508; GAPDH, D-001830). Briefly, DharmaFECT lipid (Thermo-Fisher Scientific) and siRNA were complexed with siRNAs in Opti-MEM (Gibco Life Technologies) for 20 min then mixed with cells in maintenance media. Sixteenhours following transfection, media was refreshed and 24 h following, cells were processed.
Immunoblot analysis
Whole-cell lysates were prepared in NP40 lysis buffer and protein quantification was determined by Coomassie-Bradford (Thermo-Fisher Scientific). Lysates were separated on 4-12% Nu-Page Bis-Tris gradient gels (Invitrogen, Carlsbad, CA, USA) and probed using antibodies to: Bcl-2 (Pharmingen, San Diego, CA, USA); Bid (clone no. 2D1);59 Caspase-8 (Pharmingen); Caspase-3 (Pharmingen); PCAF (SantaCruz, Dallas, TX, USA); ADA3 (SantaCruz); Phospho-Bid (S78) (Bethyl Laboratories, Montgomery, TX, USA); Phospho-Bid (S65) (Abcam, Cambridge, MA, USA); Ubiquitin P4D1 (Cell Signaling, San Diego, CA, USA); or SERPIN-B9,26 as previously described.6 Identifying and quantifying immunoblots, ImageJ software (ImageJ 1.475v, NIH, USA) was used to measure densitometry.
Immunoprecipitation
Typically 5 × 10^6 cells were lysed in 1% Triton X-100, 50 mM Tris-HCl pH 8, 300 mM NaCl, 0.02% NaAz containing protease inhibitors (Roche, Basel, Switzerland) for 30 min on ice. Insoluble debris was pelleted and lysates were pre-cleared with 1:1% slurry of sepharose beads in lysis buffer for 1 h at 4 °C. Ten micrograms of antibody for: PCAF (Bethyl Laboratories); Bid (Cell Signaling); ADA3 (SantaCruz); or IgG control, was pre-conjugated to 1:1% slurry of sepharose beads in PBS for 2 h at 4 °C. Lysate was pelleted to remove beads and 10 ul was stored as input control, and the remaining lysate was subdivided for immunoprecipitation (IP) with IgG control or specific antibody:bead conjugates. IP were performed for 16 h at 4 °C with getle agitation. Immune complexes were washed four times with wash buffer (0.1% Triton X-100, 50 mM Tris, 300 mM NaCl, 5 mM EDTA, 0.02% NaAz), once with PBS and eluted by boiling in sample buffer and separated on Nu-Page gels as described above.
Cytotoxicity assays
Generally 0.5 × 10^6 cells were treated at 37 °C with hGrzB at the specified concentration and a concentration of perforin known to induce 10% background lysis. Chromium (51Cr) and cytochrome c release assays were performed as previously described.19, 25 For flow cytometry (FACS), cells were washed with PBS containing 0.5% FCS then resuspended in the same solution with 7-AAD-FITC (Beckman Coulter, Brea, CA, USA) and 7-AAD-FITC fluorescence was detected on a cytofluorograph. Alamar blue (AB) exclusion was used according to the manufacturer's instructions (Life Technologies, Grand Island, NY, USA).
RNA isolation and first-strand cDNA synthesis
Total RNA was isolated using the RNeasy mini kit (Qiagen, Germantown, MD, USA) according to the manufacturer’s instructions. First-strand cDNA synthesis was performed using M-MLV Reverse Transcriptase (Promega) according to the manufacturer’s instructions. HPRT, GAPDH, PCAF, ADA3, Bid, Bcl-2, Bcl-w, Mcl-1, Bcl-xl, PACS2 and SERPIN-B9 genes (primers available on request) were quantified using qPCR. PCR amplification was performed using an ABI Prism-7500 (Life Technologies) with 2 pmol of each primer to 20 μl of 1 × POWER Sybergreen PCR Mastermix (Life Technologies). Annealing was at 50 °C for 2 min, denaturing at 95 °C for 10 min followed by 40 synthesis cycles at 60 °C for 1 minute.
Cell fractionation
Nuclear extracts from 2 × 10^6 cells were prepared as follows: cells were washed in ice-cold PBS, resuspended in 400 ul low salt buffer (10 mM Hepes pH 8, 1.5 mM MgCl2, 10 mM KCl, 0.5 mM DTT and 0.2 mM PMSF) on ice for 10 min, pelleted and the supernatant put aside (cytosolic extract). The nuclei were gently resuspended in 200 ul high salt buffer (20 mM Hepes pH 8, 25% glycerol, 420 mM KCl, 1.5 mM MgCl2, 0.2 mM EDTA, 0.5 mM DTT and 0.2 mM PMSF) and incubated on ice for 30 min. Debris from the nuclear extracts was removed by centrifugation. Lysate concentration was measured by Bradford assay (ThermoFisher Scientific, Rockford, IL, USA) and equal amounts of nuclear and cytoplasmic protein was loaded for immunoblot analysis.
Immunofluorescence microscopy
HeLa cells were plated in eight well chamber slides, rinsed in PBS, fixed with 4% paraformaldehyde and stained as previously described.60 Cells were stained with PCAF (Millipore, Billerica, MA, USA) and imaged as previously described.60
hGrzB activity assay
hGrzB activity was determined by the hydrolysis of synthetic peptide thiobenzylester substrate (Asp-ase activity, Boc-Ala-Ala-Asp-S-Bzl; SM Biochemicals), as previously described.1
Intracellular hGrzB staining
HeLa were treated with hGrzB (60 nM) in the absence or presence of perforin (1 nM) for the indicated times, fixed, permeabilized and stained with a PE-labeled hGrzB antibody (Clone GB11, Sanquin, Amsterdam, The Netherlands). GrzB-PE was detected on a cytofluorograph.
Statistical analysis
Results are presented as mean±S.E.M. Statistical differences for different treatments were evaluated by using t-test or one-way ANOVA with Bonferroni adjustment by Prism software (GraphPad, La Jolla, CA, USA). A P-value of <0.05 was considered significant.
Abbreviations
- hGrzB:
-
human granzyme B
- tBid:
-
truncated Bid
- KD:
-
knockdown
- MOMP:
-
mitochondrial outer membrane permeabilisation
- IAP:
-
inhibitor of apoptosis protein
- CTLs:
-
cytotoxic lymphocytes
- NK:
-
natural killer cell
- PCAF:
-
p300/CBP associated protein
- ADA3:
-
transcriptional adaptor 3
- PACS2:
-
phosphofurin acidic-cluster sorting-protein 2
- NS:
-
non-silencing
- AB:
-
Alamar blue
- NT:
-
non-targeting
- UV:
-
ultraviolet radiation
- CSP3:
-
caspase-3
- IP:
-
immunoprecipitation
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Acknowledgements
This study was supported by Program, Fellowship and Project grant support from the National Health and Medical Research Council (NHMRC) of Australia and the Cancer Council Victoria.
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Brasacchio, D., Noori, T., House, C. et al. A functional genomics screen identifies PCAF and ADA3 as regulators of human granzyme B-mediated apoptosis and Bid cleavage. Cell Death Differ 21, 748–760 (2014). https://doi.org/10.1038/cdd.2013.203
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DOI: https://doi.org/10.1038/cdd.2013.203
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