Abstract
The unfolded protein response (UPR) occurs following the accumulation of unfolded proteins in the endoplasmic reticulum (ER) and orchestrates an intricate balance between its prosurvival and apoptotic arms to restore cellular homeostasis and integrity. However, in certain neurodegenerative diseases, the apoptotic arm of the UPR is enhanced, resulting in excessive neuronal cell death and disease progression, both of which can be overcome by modulating the UPR. Here, we describe a novel crosstalk between glucocorticoid receptor signaling and the apoptotic arm of the UPR, thus highlighting the potential of glucocorticoid therapy in treating neurodegenerative diseases. Several glucocorticoids, but not mineralocorticoids, selectively antagonize ER stress-induced apoptosis in a manner that is downstream of and/or independent of the conventional UPR pathways. Using GRT10, a novel selective pharmacological modulator of glucocorticoid signaling, we describe the importance of the transrepression arm of the glucocorticoid signaling pathway in protection against ER stress-induced apoptosis. Furthermore, we also observe the protective effects of glucocorticoids in vivo in a Drosophila model of Huntington’s disease (HD), wherein treatment with different glucocorticoids diminished rhabdomere loss and conferred neuroprotection. Finally, we find that growth differentiation factor 15 has an important role downstream of glucocorticoid signaling in antagonizing ER stress-induced apoptosis in cells, as well as in preventing HD-mediated neurodegeneration in flies. Thus, our studies demonstrate that this novel crosstalk has the potential to be effectively exploited in alleviating several neurodegenerative disorders.
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Main
The unfolded protein response (UPR) occurs in response to the accumulation of unfolded and misfolded proteins in the endoplasmic reticulum (ER) and executes a coordinated sequence of intracellular events, all of which are designed to restore cellular homeostasis in the stressed cells.1, 2, 3 This is achieved by the activation of the three major arms of the UPR, involving inositol-requiring protein-1 α (IRE1α), activating transcription factor-6 (ATF6) and protein kinase RNA-like ER kinase (PERK), which in turn mediate a series of events, including the phosphorylation of an eukaryotic translation initiation factor 2α (eIF2α) to temporarily arrest protein synthesis, and generation of chaperones such as binding immunoglobulin protein (BiP) to aid in protein folding.1, 2, 3 However, when the extent of stress is overwhelming, the UPR activates the apoptotic arm, which includes the accumulation of C/EBP homologous protein (CHOP), resulting in the clearance of stressed cells.4, 5 As canonical ER stress and the UPR have been implicated in a number of disorders, including diabetes, cancer and several neurodegenerative diseases, efforts have been made to modulate specific arms of the UPR in an attempt to minimize disease pathology and improve survival.6, 7, 8, 9 This has led to the design of compounds that specifically inhibit different arms of the ER stress pathway, such as inhibitors of PERK and eIF2α dephosphorylation (salubrinal and guanabenz), IRE1α (STF-083010 and MKC-3946) and ATF6 proteolysis (AEBSF), all of which efficiently modulate specific arms of the ER stress pathway to achieve therapeutic benefits.10, 11, 12, 13, 14
Glucocorticoids exert pleomorphic effects on a variety of physiological responses and are widely prescribed to treat several inflammatory diseases, including asthma, rheumatoid arthritis and allergic rhinitis.15 Owing to their propensity to induce apoptosis, high-dose glucocorticoids are also an integral part of chemotherapy regimens in the treatment of several leukemias and lymphomas.16, 17 However, recent reports also claim specific prosurvival roles of glucocorticoids.18, 19, 20, 21 Glucocorticoid signaling involves the nuclear translocation of the dimerized ligand–receptor complex, which in turn results in the induction of several anti-inflammatory proteins as well as regulatory proteins important for metabolism. This pathway, also known as transactivation, is believed to be responsible for many of the side effects of glucocorticoids.22 Alternatively, the transrepression arm of glucocorticoid signaling involves the direct or indirect interaction of the monomeric receptor complex with specific transcription factors to regulate negatively proinflammatory gene expression, finally resulting in anti-inflammatory and immunosuppressive effects.22 In an attempt to improve the therapeutic index of these compounds, structural variants of glucocorticoids that selectively modulate the transrepression arm of glucocorticoid signaling are currently being developed.23, 24, 25, 26, 27, 28, 29, 30, 31
In the present study, we describe a novel crosstalk between glucocorticoid signaling and the apoptotic arm of the UPR, highlighting the potential use of glucocorticoid therapy in several disease conditions. Using a selective modulator of glucocorticoid signaling, GRT10, we further establish a role for the transrepression arm of glucocorticoid signaling in the inhibition of ER stress-induced apoptosis. Furthermore, using microarray analysis, we identify growth differentiation factor 15 (GDF15) as a major player in ER stress-induced apoptosis that can be effectively downregulated by the transrepression arm of glucocorticoid signaling. Finally, as ER stress has a central role in several neurodegenerative diseases, we exploit a fruit fly model of Huntington’s disease (HD), which expresses a fragment of mutant huntingtin (HTT) – the causative protein in this disorder – to validate the therapeutic potential of glucocorticoid signaling and GDF15 in HD, and find that this novel crosstalk could be an important target for designing drugs to alleviate several neurodegenerative diseases.
Results
Mometasone furoate antagonizes ER stress-induced apoptosis with little changes to the major arms of the UPR
Following a series of screening experiments, we found that treatment with mometasone furoate, a glucocorticoid commonly used to treat inflammation of the skin and airways, resulted in significant protection against apoptosis induced by several conventional ER stressors, including tunicamycin, brefeldin A (BFA), thapsigargin (THG) and dithiothreitol (DTT) (Figure 1a). This protective effect was comparable with that of the α-2 adrenergic receptor agonist (guanabenz).11 Both mometasone and guanabenz also protected against tunicamycin-induced activation of caspase-9, -7 and -3, as well as cleavage of the canonical caspase substrate (poly (ADP-ribose) polymerase (PARP)) (Figure 1b). The protective effect of guanabenz against ER stress-induced apoptosis has been attributed to its ability to sustain the phosphorylation status of eIF2α, which in turn resulted in a temporary block of global translation and modulation of the major arms of the UPR.11 As mometasone was equally efficacious in antagonizing ER stress-induced apoptosis, we speculated that the protective effects of mometasone, similar to guanabenz, could be due to changes in phospho-eIF2α levels and other arms of the UPR. However, mometasone failed to alter most UPR-mediated RNA and protein changes (Figures 1c and d), despite fluctuating levels of the phosphorylated form of eIF2α (Figure 1d). In contrast, guanabenz mediated sustained levels of phospho-eIF2α and inhibited CHOP accumulation (Figure 1d), thereby suggesting that the protective effects of mometasone could be distinct from those of guanabenz.
Glucocorticoid receptor signaling is critical for the antiapoptotic functions of glucocorticoids
The protective effect of mometasone was more restricted to cell death induced by canonical ER stressors than other cell death stimuli, as the extent of rescue from apoptosis in cells exposed to common cell death stimuli, such as the kinase inhibitor (staurosporine), the BCL-2 inhibitor (ABT-263), the proteasomal inhibitor (MG132), the translation inhibitor (cycloheximide) or an oxidizing agent (hydrogen peroxide (H2O2)), was not statistically significant (Figure 2a), The protective effects of mometasone gradually diminished over time, suggesting that repeated administration of mometasone may be required to prolong the protective response (Figure 2b). In most of these experiments, mometasone was added to the cells at least 4 h before tunicamycin treatment, to ensure complete activation of the glucocorticoid receptor signaling pathways. However, altering the exposure times to mometasone yielded similar protection against tunicamycin-induced apoptosis (Figure 2c), thus suggesting that preconditioning of cells is not a requirement for efficient inhibition of the apoptotic arm of the UPR. Furthermore, the ability to antagonize ER stress-induced apoptosis was not restricted to mometasone, as several glucocorticoids, but not mineralocorticoids, also diminished tunicamycin-mediated apoptosis (Figure 2d). To attribute these protective effects of glucocorticoids to elevated glucocorticoid receptor signaling, we used a competitive inhibitor (mifepristone),32 which completely reversed the antiapoptotic effects of budesonide and dexamethasone but not those of fluticasone and mometasone (Figure 2e). This was most likely due to the high association and low dissociation rate constants of fluticasone and mometasone with the glucocorticoid receptor.33 Furthermore, silencing the cellular expression levels of the glucocorticoid receptor completely abolished the ability of mometasone to protect against tunicamycin-induced apoptosis (Figure 2f), thus confirming the existence of a crosstalk between glucocorticoid receptor signaling and ER stress-induced apoptosis.
The transrepression arm of glucocorticoid signaling antagonizes ER stress-induced apoptosis and ameliorates mutant HTT-mediated neurodegeneration
To determine the relative contributions of transactivation and transrepression pathways to the protective effects, we used a novel and selective modulator of glucocorticoid signaling (GRT10), which is unable to transactivate effectively glucocorticoid-dependent gene expression but demonstrates full transrepression activity (Figure 3a).29 Similar to mometasone, GRT10 exhibited antiapoptotic effects, even at nanomolar concentrations (Figure 3b), thus demonstrating that the transrepression arm of glucocorticoid signaling is sufficient to inhibit ER stress-induced apoptosis. As ER stress-induced apoptosis is linked to the incidence of several neurodegenerative diseases, we wished to assess whether glucocorticoids could ameliorate neurodegeneration in a relevant in vivo model. Therefore, we used a Drosophila model of HD, in which flies pan-neuronally express a mutant HTT exon 1 fragment (HTT93Q) and exhibit a progressive loss of photoreceptor neurons (rhabdomeres) during their lifespan.34, 35, 36, 37, 38 Treatment of adult HD flies with either mometasone or GRT10 markedly rescued mutant HTT-mediated neurodegeneration, although the extent of protection with GRT10 was slightly lower than with mometasone (Figures 3c and d). Moreover, treatment of the glucocorticoids during larval stages reduced neurodegeneration in newly emerged (day 0) HD flies (Figure 3e), suggesting that the transrepression arm of glucocorticoid signaling is neuroprotective both in adult HD flies and during development.
GDF15 has a critical role in regulating the antiapoptotic functions of glucocorticoids
To identify the target downstream of the transrepression arm that was critical for neuroprotection, we performed microarray analysis following tunicamycin in the presence of mometasone or GRT10. While mometasone or GRT10 exhibited varied effects on tunicamycin-mediated transcriptional changes, only a limited subset of genes induced by tunicamycin was maximally decreased by both mometasone and GRT10 (Figure 4a). Of these genes, the induction of GDF15 following ER stress, which was efficiently diminished both at RNA and protein levels by mometasone and GRT10 (Figure 4b), was intriguing, as we observed a similar, time-dependent induction of GDF15 in immortalized rat adrenal gland phaeochromocytoma PC12 cells expressing a mutant HTT fragment (Figure 4c).39 To assess if GDF15 upregulation was responsible for ER stress-induced apoptosis, we used RNA interference to silence the expression of GDF15 in cells. Following silencing of GDF15, tunicamycin-induced apoptosis was significantly reduced (Figure 4d), thus confirming the involvement of GDF15 in ER stress-induced apoptosis. Furthermore, exposure of GRT10 to cells that lack GDF15 did not significantly enhance the protection (Figure 4d), thus suggesting that GDF15 was one of the major and crucial targets of GRT10, with respect to their antiapoptotic functions. These observations were further confirmed in vivo, wherein genetic inhibition of maverick (mav) – the Drosophila ortholog of GDF15 – by RNAi efficiently ameliorated HD-relevant photoreceptor loss at day 7 (Figure 4e). Interestingly, we observed a significant age × genotype interaction (F(1,46)=7.76, P<0.01), suggesting that knockdown of mav is not only neuroprotective but slows progression of neuron loss in this animal model (Figure 4e). Finally, in agreement with our findings in cells, further treatment of GRT10 on flies lacking mav failed to enhance the extent of protection from baseline (Figure 4f), confirming that mav is a critical target of the transrepression arm of glucocorticoid receptor signaling. Taken together, our data suggest that the transrepression arm of glucocorticoid signaling ameliorates mutant HTT-mediated neurodegeneration (Figure 5), and this novel crosstalk between GDF15 and the transrepression arm of glucocorticoid signaling could be an important target in the design of drugs to alleviate other neurodegenerative disorders in addition to HD, including Alzheimer’s and Parkinson’s diseases.
Discussion
HD is a neurodegenerative disorder characterized by psychological problems, cognitive dysfunction, motor impairment and ultimately death. A prominent feature of HD pathology is the assembly of mutant HTT protein into cytoplasmic- and nuclear-insoluble amyloid-like fibrils, as well as soluble oligomeric species.40 Although the disease pathology has been attributed to a mutation in the HTT gene, resulting in the expansion of the polyglutamine tract in the HTT protein,41 the molecular mechanisms underlying mutant HTT toxicity are poorly understood. However, a number of pathological mechanisms, including mitochondrial dysfunction42, 43 and ER stress7, 44 have been implicated in HD. Currently, there is no cure for HD, with drugs only available for symptomatic treatment.45
Canonical ER stress response and the UPR have been implicated in a wide variety of disorders, including diabetes, cancer, inflammatory diseases and neurodegeneration.6 Although an enhanced activation of the survival arms of the UPR is observed in cancers, the apoptotic pathways are more prevalent in several neurodegenerative diseases.6, 9 UPR-targeted gene manipulation, such as the deletion of the proapoptotic protein CHOP, has been observed to delay disease onset and improve survival in Parkinson’s disease and amyotrophic lateral sclerosis.46, 47 ER stress-induced apoptosis has been implicated as an important contributor to cellular toxicity in several models of HD.48, 49, 50, 51, 52, 53 Furthermore, other UPR modulators (such as salubrinal, several chemical chaperones, overexpression of GRP78 and downregulation of SCAMP5, Rrs1 and ASK1) have been successfully used in alleviating HD-associated phenotypes.10, 49, 52, 54, 55, 56, 57, 58, 59, 60 Recently, guanabenz, an α-2 adrenergic receptor agonist, was reported to exhibit significant protection against apoptosis via selective modulation of the UPR.11 Unlike guanabenz, which antagonizes ER stress-induced apoptosis by blocking most UPR pathways, glucocorticoids exert their protective effects downstream of and/or independent of the major arms of the UPR (Figures 1 and 2).
Although previous studies have reported protective roles for glucocorticoids in cell death,61, 62, 63 our current study characterizes a role for transrepression and not transactivation in the glucocorticoid-mediated protection against ER stress-induced apoptosis (Figure 3). Notably, we have identified, characterized and validated a role of GDF15 in ER stress-induced apoptosis (Figure 4). The ability of glucocorticoids to repress the transcription of GDF15, in a manner that is partly dependent on the Brm Swi/Snf ATPase, has been documented.64 Furthermore, downregulation of Brm increased glucocorticoid receptor occupancy most likely by competing with the glucocorticoids to bind the receptor, thus modulating GDF15 transrepression.64 This raises the possibility that inactivation of Brm ATPase may abrogate the protective effects of glucocorticoids against ER stress-induced apoptosis and provide further mechanistic insight to the regulation of GDF15.
GDF15 (also known as NAG1 or MIC1) is a member of the TGF-β superfamily of proteins and has been implicated in various pathologies including inflammation, cancer, cardiovascular diseases and obesity.65 In the central and peripheral nervous systems, GDF15 is widely expressed and is particularly induced in neuronal lesions and cerebral ischemia.66 However, the role of GDF15 in disease pathologies associated with CNS lesions is further complicated by the progressive loss of neurons, motor axons and rotarod skills in GDF15-deficient mice, suggesting the importance of GDF15 in neuronal health.67 In this study, we observe that GDF15 is upregulated under stress conditions, and normalization of its function via RNA interference or pharmacological inhibition resulted in a marked protection against both ER stress-induced apoptosis and HD-associated neurodegeneration (Figure 4). Thus, typical of several members of the TGF-β superfamily, GDF15 performs opposing functions in disease conditions, and hence further studies are required to identify specific molecular functions of this protein.65 Nevertheless, our study conclusively demonstrates that the novel crosstalk between GDF15 and the transrepression arm of glucocorticoid signaling could be an important target in the design of drugs to alleviate several neurodegenerative disorders, including HD, Alzheimer’s and Parkinson’s diseases.
Materials and Methods
Cell culture
HeLa cells from ATCC (LGC Standards, Middlesex, UK) were cultured in DMEM medium supplemented with 5 mM l-glutamine and 10% fetal calf serum (FCS) (all from Life Technologies Inc., Paisley, UK). PC12 cells, stably transfected with a ponasterone A-inducible mutant HTT fragment (103Q) fused with green fluorescent protein (GFP)39 (kindly provided by Leslie Thompson, University of California, Irvine, Irvine, CA, USA), were cultured in DMEM supplemented with 10% horse serum (v/v), 5% FCS (v/v), 2 mM GlutaMax and 1 mM sodium pyruvate (all from Life Technologies).
Drosophila transgenic lines
Flies were raised on standard maize media, in LD 12:12 at 25 °C. The elavGAL4 [c155] driver and y1scv1; P{TRiP.GL01025}attP40 were obtained from the Bloomington Stock Center (Bloomington, IN, USA). The w;+;UAS HTT93Q exon 1 flies were a gift from Larry Marsh and Leslie Thompson (University of California, Irvine, Irvine, CA, USA).34
Reagents and antibodies
All steroids (mometasone furoate, dexamethasone, fluticasone propionate, budesonide and deoxycorticosterone acetate) and ER stress agents (tunicamycin, BFA, THG and DTT) were from Tocris Bioscience (Bristol, UK). Guanabenz was kindly provided by Medical Research Council Technology (London, UK). Ponasterone A was from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against glucocorticoid receptor, phospho- and total eIF2α from Cell Signaling Technology Inc. (Danvers, MA, USA), PARP from Alexis Biochemicals (Nottingham, UK), tubulin from Calbiochem (Darmstadt, Germany), GDF15, BiP and CHOP from Abcam (Cambridge, UK) and caspase-3, -7 and -9 (a kind gift from Dr Xiao-Ming Sun, MRC Toxicology Unit, Leicester, UK) were used. All other reagents, unless mentioned otherwise, were from Sigma-Aldrich Co. (St. Louis, MO, USA).
Apoptosis measurements and immunoblotting
Apoptosis was assessed by phosphatidylserine (PS) externalization as described previously.68 Western blots were carried out according to standard protocols. Briefly, 30 μg of total protein lysate (lysed in a buffer of 20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% (v/v) Triton X-100, 1 mM EDTA, 30 mM NaF, 1 mM glycerolphosphate, 1 × proteinase inhibitor (Roche, West Sussex, UK) and 1 mM Na3VO4) was denatured in sampling buffer (50 mM Tris-HCl, pH 6.8, 10% (w/v) glycerol, 2% (w/v) SDS, 0.02% (w/v) bromophenol blue and 2.5% (v/v) β-mecaptoethanol) and subjected to SDS-PAGE. Proteins were transferred to nitrocellulose membranes, blocked with 5% (w/v) milk in Tris-buffered saline Tween-20 and incubated with various primary antibodies. Washed membranes were incubated with horseradish peroxidase-conjugated secondary antibody and protein bands visualized with ECL reagents (GE Healthcare, Bucks, UK).
RT-PCR and XBP-1 splicing
Total RNA extracted (RNAeasy; Qiagen, Hilden, Germany) from cells was reverse-transcribed (Invitrogen, Carlsbad, CA, USA), and the resulting cDNA was used as a template for PCR amplification using primers 5′-TTACGAGAGAAAACTCATGGC-3′ and 5′-GGGTCCAAGTTGTCCAGAATGC-3′ to generate the 289 bp amplicon of spliced XBP-1, which was resolved on a 2.5% agarose/1 × TAE gel and visualized.
siRNA knockdowns
HeLa cells were reverse-transfected with 10 nM siRNA of control, glucocorticoid receptor (s6187) or GDF15 (sc-39798) obtained from Ambion (Carlsbad, CA, USA) or Santa Cruz Biotechnology using Interferin Reagent (Polyplus Transfection Inc., New York, NY, USA) according to the manufacturer's protocol and processed 72 h after transfection.
Compound treatment of Drosophila
Fly food medium was liquefied by gentle heating and mixed thoroughly with appropriate glucocorticoid concentrations. Newly emerged flies were transferred to vials containing food mixed with the glucocorticoid and maintained for 7 days, with the food changed daily to ensure a constant drug supply. For rhabdomere analysis at day 0, flies were crossed and grown on standard maize medium supplemented with 10 μM of either glucocorticoid.
Pseudopupil analysis
Treated flies were anaesthetized with CO2 and the heads removed. The heads were mounted face-up on microscope slides and viewed using a Nikon Optiphot-2 microscope (Nikon UK Ltd, Surrey, UK) at × 40 magnification. Approximately 50 ommatidia were counted from about 12 flies per treatment and averaged to acquire the number of rhabdomeres per ommatidium. Statistical analyses were conducted by ANOVA with Newman–Keuls post hoc tests.
Microarray analysis and statistics
Total RNA extracted from HeLa cells exposed to tunicamycin (10 μM) for 20 h, followed by a 4 h pretreatment with mometasone (1 μM) or GRT10 (0.1 μM), was used to make biotin-labeled cRNA using the Illumina TotalPrep RNA Amplification Kit (Ambion). The resulting cRNA was hybridized to an Illumina HumanHT-12 BeadChip array (Illumina, San Diego, CA, USA), Cy3 labeled and scanned using an Illumina BeadArray Reader (Illumina). Microarray data normalization and analyses were carried out as before.69 For heatmap analysis, the top 50 genes with the highest fold changes (P≤0.003), followed by tunicamycin treatment, were compiled. The transcriptional changes of those genes in the treated cells were compared using MultiExperiment Viewer. Microarray analysis on PC12 cells exposed to DMSO or ponasterone A for 12, 24 and 72 h were carried out as before, but the samples were hybridized to Illumina rat RatRef-12 v1 BeadChip microarrays instead and the microarray data sets for different treatments were compared with the top 20 differentially expressed genes of tunicamycin treatments.
Abbreviations
- ATF6:
-
activating transcription factor-6
- BiP:
-
binding immunoglobulin protein
- BFA:
-
brefeldin A
- CHOP:
-
C/EBP homologous protein
- DTT:
-
dithiothreitol
- eIF2α:
-
eukaryotic translation initiation factor 2α
- ER:
-
endoplasmic reticulum
- GDF15:
-
growth differentiation factor 15
- HD:
-
Huntington’s disease
- HTT:
-
huntingtin
- IRE1α:
-
inositol-requiring protein-1 α
- PERK:
-
protein kinase RNA-like ER kinase
- THG:
-
thapsigargin
- UPR:
-
unfolded protein response
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Acknowledgements
We thank the Medical Research Council Toxicology Unit for core support, and the CHDI Foundation Inc. for funding (to FG). We are also grateful to MRCT for guanabenz, Dr. Sun for the caspase-3, -7 and -9 antibodies and Drs. Thompson and Marsh for the HTT PC12 cells and fruit flies.
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Varadarajan, S., Breda, C., Smalley, J. et al. The transrepression arm of glucocorticoid receptor signaling is protective in mutant huntingtin-mediated neurodegeneration. Cell Death Differ 22, 1388–1396 (2015). https://doi.org/10.1038/cdd.2015.1
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DOI: https://doi.org/10.1038/cdd.2015.1
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