Abstract
Programmed cell death, which is required for the development and homeostasis of metazoans, includes mechanisms such as apoptosis, autophagic cell death, and necrotic (or type III) death. Members of the Bcl2 family regulate apoptosis, among which Bax and Bak act as a mitochondrial gateway. Although embryonic fibroblasts from Bax/Bak double-knockout (DKO) mice are resistant to apoptosis, we previously demonstrated that these cells die through an autophagy-dependent mechanism in response to various types of cellular stressors. To determine the physiological role of autophagy-dependent cell death, we generated Atg5/Bax/Bak triple-knockout (TKO) mice, in which autophagy is greatly suppressed compared with DKO mice. Embryonic fibroblasts and thymocytes from TKO mice underwent autophagy much less frequently, and their viability was much higher than DKO cells in the presence of certain cellular stressors, providing genetic evidence that DKO cells undergo Atg5-dependent death. Compared with wild-type embryos, the loss of interdigital webs was significantly delayed in DKO embryos and was even further delayed in TKO embryos. Brain malformation is a distinct feature observed in DKO embryos on the 129 genetic background, but not in those on a B6 background, whereas such malformations appeared in TKO embryos even on a B6 background. Taken together, our data suggest that Atg5-dependent cell death contributes to the embryonic development of DKO mice, implying that autophagy compensates for the deficiency in apoptosis.
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Main
Programmed cell death (PCD) is a genetically regulated mechanism that is essential for diverse biological events such as morphogenesis and the elimination of harmful cells.1 Apoptosis is the major physiological mechanism mediating PCD, and is regulated by members of the Bcl2 family.2 However, PCD is also mediated by other mechanisms, such as autophagic cell death or type II PCD.3, 4
Macroautophagy (hereafter referred to as autophagy) is an intrinsic cellular process that digests cellular components. Autophagy occurs constitutively at low basal levels and is accelerated by diverse cellular stressors. This machinery is driven by at least 30 autophagy-related proteins (Atgs) that are highly conserved from yeast to mammals.5, 6 Evidence indicates that among these molecules, Atg5 and Atg7 are essential for autophagy.6 But we previously discovered that in addition to Atg5/Atg7-dependent autophagy, there an Atg5/Atg7-independent mechanism of autophagy exists in mammalian cells.7
Autophagy is primarily a prosurvival mechanism. Therefore, it is frequently activated to suppress cell damage, when cells are subjected to severe stress. However, evidence suggests that autophagy contributes to cell death under certain conditions,8 and is therefore sometimes referred to as ‘autophagic cell death’. In this context, this term is used only when nonapoptotic cell death occurs via the activation of autophagy, and cell death is rescued by the suppression of autophagy.9 In Drosophila, the programmed death of larval salivary gland cells is proposed to be a good example of autophagic cell death because ecdysone acts to remove the salivary glands via the transcriptional upregulation of autophagy genes.10, 11 Furthermore, the genetic ablation of autophagy genes in Drosophila delays the elimination of salivary glands. Despite findings that the effects of autophagy genes are partial and that the apoptotic machinery appears to contribute to some extent, the PCD of salivary glands is described as autophagic cell death.
In mammals, we previously demonstrated that embryonic fibroblasts from Bax/Bak double-knockout (DKO) mice, in which the apoptosis machinery is blocked, die containing numerous autophagic structures after exposure to a variety of apoptotic stimuli. Furthermore, this type of cell death occurs through autophagy because it is prevented by autophagy inhibitors or by silencing of the expression of autophagy genes.12 Moreover, accumulating evidence indicates that autophagic cells are committed to undergo cell death under certain conditions.13 Although a large body of evidence suggests that mammalian cells undergo autophagic cell death, such data were acquired mainly from studies on cultured cells, and hence there is only limited evidence demonstrating autophagic cell death in vivo. To obtain genetic evidence of the occurrence of autophagic cell death during development or in the stress responses of mammals in vivo, we generated Atg5/Bax/Bak triple-knockout (TKO) mice and compared their phenotypes with those of Bax/Bak DKO mice because autophagic cell death is readily observed in apoptosis-resistant cells. The data presented here suggest that autophagic cell death contributes to the normal formation of the interdigital web and may be involved in embryonic viability and brain development in apoptosis-deficient mice, implying that autophagic cell death contributes to development by compensating for a deficiency in apoptosis.
Results
Generation of Atg5/Bax/Bak TKO mice
We previously reported that mouse embryonic fibroblasts (MEFs) from Bax/Bak DKO mice die by autophagic cell death after exposure to various stressors.12 However, to our knowledge, there are no published studies that present genetic evidence of mammalian autophagic cell death in vivo. Therefore, we generated Atg5/Bax/Bak TKO embryos by breeding Bax+/−Bak−/− mice with Atg5+/− mice.14 We further generated MEFs from wild-type (WT), Atg5 KO, DKO, and TKO embryos.
To detect the activation of autophagy and to determine the mechanism of autophagic cell death, MEFs were first treated with etoposide, a DNA-damaging reagent. The activation of autophagy was indicated by the punctate distribution of green-fluorescent protein (GFP)-tagged microtubule-associated protein light chain 3 (LC3) and by the production of lipid conjugates of LC3 (LC3-II). WT, but not Atg5 KO MEFs, exhibited a significant levels of autophagy (Figures 1a–c). In DKO MEFs, the number of punctate GFP-LC3 cells and the extent of LC3-II formation were greater than those of WT MEFs (Figures 1a–c). In contrast, LC3 modifications were undetectable in TKO MEFs (Figures 1a–c). Electron microscopic (EM) analysis confirmed the presence of autophagic vacuoles in etoposide-treated WT MEFs (Figures 1d and e ‘WT’). In DKO MEFs, the extent of autophagy was largely increased as judged from the bigger size and the increased number of autolysosomes (Figures 1d and e ‘DKO’), which is consistent with the LC3 modifications (Figures 1a–c). In TKO MEFs, we only observed a few small autophagic vacuoles (Figures 1d and e ‘TKO’). The increase and decrease of autophagy in DKO MEFs and TKO MEFs, respectively, were also verified functionally using a long-lived protein degradation assay (Figure 1f). Furthermore, consistent results were obtained using an independent set of DKO and TKO MEFs (Supplementary Fig. 1a). Note that although we recently identified an Atg5-independent autophagic pathway,7 TKO MEFs, unlike Atg5 KO MEFs, exhibited limited autophagy (Figure 1d ‘TKO’ and Supplementary Fig. 1b and c), suggesting that Atg5-independent alternative autophagy was largely suppressed in the absence of Bax and Bak expression.
Viability of TKO and DKO MEFs
Because etoposide strongly induced autophagy in DKO MEFs, whereas the induction was greatly decreased in TKO MEFs, we reasoned that if there were more viable TKO MEFs compared with DKO MEFs, Atg5 likely contributed to etoposide-induced cell death. Therefore, we treated MEFs with etoposide and determined their viability. The viability of WT MEFs rapidly decreased, as assessed using the CellTiter-Blue (CTB) assay (Figure 2a). Morphologically, cells were rounded and detached from the culture dish 12 h after etoposide treatment (Figure 2b). Cell death was suppressed by zVAD-fmk, a broad caspase inhibitor, indicating that apoptosis accounts for the death of these cells (Supplementary Fig. 2a).
DKO MEFs were resistant to apoptosis such that zVAD-fmk did not affect the level of cell death (Supplementary Fig. 2b); however, etoposide still decreased their viability (Figure 2a). Morphological alteration and detachment began from 12 h (Figure 2b), when greater than 60% of the cells contained GFP-LC3 puncta (Figure 1b). TKO MEFs were more resistant to death (Figure 2a), and hence their detachment occurred at a later time point (Figure 2b). The higher viability of TKO MEFs compared with DKO MEFs was confirmed by cell growth (Figure 2c) and colony formation assays (Figure 2d). Similar results were obtained using an independent set of MEFs (Supplementary Fig. 2c) and when MEFs were treated with staurosporine (STS), a pan-kinase inhibitor (Figure 2e). Therefore, TKO MEFs were more resistant to cell death induced by etoposide and STS compared with DKO MEFs.
We obtained consistent results upon the silencing of Atg5 expression in DKO MEFs, indicating the involvement of Atg5 in the death of DKO MEFs (Figures 2f and g, and Supplementary Fig. 2d). Because the major function of Atg5 is to regulate autophagy, we reasoned that autophagy contributed to the stress-induced death of DKO MEFs. This assumption was supported by the findings that the etoposide-induced cell death of DKO MEFs was suppressed to a level similar to that of TKO MEFs when cultures were exposed to the inhibitor of autophagy 3-methyladenine (3-MA) (Figure 2h), which did not inhibit the death of TKO MEFs (Figure 2h). Consistent results were obtained when autophagic protein degradation was blocked by lysosomal inhibitors (Supplementary Fig. 2e). Furthermore, silencing of the expression of Beclin-1 (Supplementary Fig. 2f, g), which is an essential molecule for autophagy, inhibited etoposide-induced death of DKO MEFs, but not TKO MEFs (Figures 2i and j). These data indicated that Atg5/Beclin-1-dependent autophagy is required for the stress-induced death of DKO MEFs.
We further examined the possible involvement of other modes of death in DKO and TKO MEFs. These MEFs were not rescued by nec-1 (a necroptosis inhibitor),15 ouabain (a Na/K ATPase inhibitor that suppresses autosis),16 cyclosporine A (a Cyclophilin D inhibitor that suppresses mitochondria membrane permeability transition-mediated necrosis),17 and DPQ (a PARP inhibitor that suppresses PARP-dependent necrosis)18 (Supplementary Fig. 3a, b). Furthermore, lysosomal membrane permeabilization (LMP)-mediated cell death19 was not observed because acridine-orange staining (an LMP marker) revealed that puncta formation of acidic compartments was maintained even after etoposide treatment (Supplementary Fig. 3c). Taken together, these data indicate that autophagic cell death, but not apoptosis, necroptosis, autosis, PARP-dependent necrosis, MPT-mediated necrosis, or LMP-mediated cell death, is involved in the stress-induced death of DKO MEFs.
Morphology of etoposide-treated TKO MEFs
Although TKO MEFs were resistant to apoptosis and autophagic cell death, they still lost their viability when exposed to etoposide. Therefore, to gain some insight into the mechanism of this death, we performed comparative EM analysis of DKO and TKO MEFs. The most prominent difference observed was hyperactivated autophagy in DKO MEFs and its absence in TKO MEFs (Figures 3a and b). Furthermore, the nuclear membranes of DKO MEFs were intact (Figure 3a), whereas those of TKO MEFs were severely distorted and disrupted (Figures 3c and d). Staining of the nuclear membrane confirmed this distortion (Figure 3e). The disruption of the nuclear membrane was evident because some mitochondria were observed within the nuclear matrix surrounded by the nuclear envelope, which was confirmed by the close localization of the nucleolonema (Figure 3d). Simple deformation of the nuclear membrane does not explain these observations because nuclear membrane normally separate the mitochondria and nuclear compartment. These observations are consistent with the effects of DNA-damaging agents reported by others.20
Extensive attachment of heterochromatin to the nuclear membrane of TKO MEFs was observed (Figures 1d and 3b). This morphology was different from that of apoptotic cells, in which the heterochromatin was not tightly associated with the nuclear membrane. Other organelles appeared undamaged (Supplementary Fig. 4). The population of cells with abnormal nuclei was similar compared with that of dead TKO cells (Figure 2a). Therefore, although we do not have any evidence that the death of TKO MEFs can be attributed to the disruption of the structure of the nucleus, we consider that this is one reasonable mechanism of TKO cell death.
Death of DKO and TKO thymocytes
We next examined whether primary cells undergo Atg5-dependent cell death. For this purpose, we isolated thymocytes from Bak KO, DKO, and TKO embryos. Note that Bak KO mice do not demonstrate any abnormalities because of the presence of Bax. We first analyzed thymocyte development using the T-cell markers CD4 and CD8. Although the number of single-positive thymocytes increases in adult DKO mice,21 most thymocytes from embryos of each genotype were uniformly CD4+CD8+ (Supplementary Fig. 5a).
We next determined the cellular response to STS. Bak KO thymocytes showed a rapid loss of viability (Figure 4a). Compared with Bak KO thymocytes, DKO thymocytes showed less cell death, and TKO thymocytes showed even less cell death (Figure 4a). Consistent results were obtained when cells were treated with different dose of STS (Supplementary Fig. 5b). To investigate the mechanism of cell death, we performed EM observation of the dead thymocytes of each genotype. We classified the cells according to their morphological characteristics as follows: apoptotic (pyknotic cells with condensed chromatin), dissociation of outer and inner nuclear membranes, hyperactivated autophagy (resembling etoposide-treated DKO MEFs (Figure 3a)), and disrupted nuclear membrane (resembling etoposide-treated TKO MEFs (Figure 3b)) (Figure 4b). Among the four morphologically distinguishable features of dead cells, apoptotic cells predominated in Bak KO thymocytes (Figure 4c); this was confirmed by the inhibition of cell death by zVAD-fmk (Supplementary Fig. 5c). The death of most DKO thymocytes was characterized by hyperactivated autophagy (Figure 4c), which was supported by the induction of LC3-II (Supplementary Fig. 5d). Suppression of DKO thymocyte death by 3-MA but not by z-VAD-fmk (Figure 4d) suggested that the induction of autophagy was the cause of cell death. The death of TKO thymocytes was characterized by notable disruption of the nuclear membrane, and the number of autophagic cells was reduced compared with DKO thymocytes. These results suggest that major types of cell death in Bak KO, DKO, and TKO thymocytes were apoptosis, cell death with hyperactivated autophagy, and cell death with nuclear membrane disruption, respectively. Because the majority of DKO thymocytes died with autophagic characteristics (Figure 4c) and autophagy-resistant TKO thymocytes were more viable than DKO thymocytes (Figure 4a), we conclude that autophagy contributes to the cell death induced by STS.
Unlike STS treatment, X-ray irradiation induced only a small level of autophagy in DKO thymocytes (equivalent to that of TKO thymocytes) (Figure 4e) and weakly induced cell death to an equivalent extent to that of TKO thymocytes (Figure 4f). Similar results were obtained when DKO and TKO thymocytes were exposed to etoposide and an anti-CD3 antibody (Figures 4g and h), indicating that the mode of cell death was stimulus-dependent.
Autophagy-dependent PCD in the interdigital web
To determine whether Atg5-dependent cell death is involved in embryonic development, we generated DKOs and TKOs from mice on a C57BL/6J genetic background by repeated backcrossing, because several cell death-associated phenotypes are affected by genetic background.22
The loss of interdigital cells represents an example of PCD that is mediated by apoptosis.23 We observed many TUNEL-positive cells in the interdigits of Bak KO embryos (Supplementary Fig. 6a and b). EM observation confirmed the presence of apoptotic nuclei (Figure 5a), many of which were engulfed by macrophages (Figure 5a). However, even in Apaf1 or Caspase9 KO mice, which are resistant to apoptosis, disappearance of the interdigital web occurs with some delay. We also observed this delay in Bax/Bak DKO embryos (Figure 5b,Supplementary Fig. 6c). Quantitative analysis of the area of elimination of the interdigital web per palm area (see Methods and Supplementary Fig. 7) confirmed the slower removal of the interdigital web of DKO embryos than Bak KO embryos at E14.5 and E15.5 (Figure 5c). Note that we analyzed interdigital web clearance only using littermate Bak KO, DKO, and TKO embryos, because accurate estimation of the impregnation times of mice is difficult, and a difference of half a day results in a large difference at that stage. To determine the mechanism of disappearance of the interdigital web from DKO embryos, we performed EM observation and found that the majority of interdigital web cells contained a number of autolysosomes (Figure 5a). Moreover, some cells appeared to be destroyed because they were filled with autolysosomes, as shown in Figure 5a. These cells resemble with etoposide-treated DKO MEFs and STS-treated DKO thymocytes, suggesting autophagic cell death. The presence of numerous LC3-positive puncta (Figure 5d) and the absence of TUNEL-positive cells (Supplementary Fig. 6b) among the interdigital web cells support this conclusion.
In TKO embryos, loss of the interdigital web was further delayed in the absence of autophagy (Figures 5b and c,Supplementary Fig. 6c), suggesting that Atg5-dependent cell death contributed to the elimination of interdigital webs. Interdigital cells of TKO embryos contained only a few autophagic vacuoles but had patchy chromatin structure and disrupted nuclear membranes (Figure 5a) similar to those of etoposide-treated TKO MEFs and STS-treated TKO thymocytes. These data suggest that autophagic cell death plays a crucial role in the embryonic development of apoptosis-resistant mice.
Apoptotic cells are quickly removed by macrophages via a mechanism that is dependent on the exposure of phosphatidyl serine (PS) on the cell membrane,24 as was observed in tissue sections of the interdigital webs of Bak KO embryos (Figure 5a). However, we did not observe macrophages in the interdigital webs of DKO and TKO embryos (Figure 5a), probably because PS exposure was not actively induced in dying DKO and TKO cells. Furthermore, PS exposure was delayed significantly in etoposide-treated DKO and TKO MEFs (Supplementary Fig. 8a and b), and these cells were not engulfed by macrophages (Supplementary Fig. 8c and d). In the interdigital webs of DKO and TKO embryos, dying cells should be eliminated by shedding, and not by macrophage engulfment.
Abnormal embryonic death and brain development of Atg5/Bax/Bak TKO embryos
Finally, we examined the other phenotypes of DKO and TKO embryos. When we counted the number of viable embryos on various embryonic day, the population of TKO embryos, but not DKO embryos, was ~50% lower than the expected Mendelian rate (Figure 6a). Consistently, we observed dead TKO embryos on each embryonic day (Figure 6b). Because a large number of dead embryos were observed on E13.5 and then gradually decreased, and as all the dead embryos exhibited organogenesis failure with some of them beginning to disintegrate (Figure 6c). TKO embryos seemed to die before E13.5 and then be reabsorbed by the uterus. These data indicated that DKO embryos developed normally, whereas TKO embryos are prone to embryonic death.
In addition to interdigital web elimination and embryonic death, we also observed exencephaly in some, but not all, TKO embryos (Figure 6d). Exencephaly is a common phenotype of apoptosis-deficient mice,23 which develops because of incomplete neural tube closure caused by a dysregulation of apoptosis,25 and largely depends on the mouse strain. For example, exencephaly occurs in the 129 strain but only rarely in the C57BL/6J strain.22 We consistently observed exencephaly in only 3.6% of DKO embryos on a C57BL/6J background compared with 0.6% of WT embryos, and this difference was not statistically significant (Figure 6e). In contrast, we observed exencephaly in ~40% of TKO embryos on a C57BL/6J background (Figure 6e), which showed a statistically significant difference from DKO embryos. The morphology is similar to the exencephaly observed in Apaf1 KO embryos on a 129 background.26 These data, taken together with the absence of detectable exencephaly in Atg5 KO embryos, suggest that brain development in DKO embryos proceeds normally, probably by the compensation of autophagic cell death for the apoptosis deficiency on a C57BL/6J background. Therefore, a simultaneous loss of apoptosis and autophagic cell death may increase the rate of exencephaly (see Discussion). Taken together, we suggest that in apoptosis-deficient mice, autophagic cell death partly mediates developmental cell death by compensating for the deficiency in apoptosis.
Discussion
For many years, physiological cell death was thought to be caused by apoptosis. However, certain types of nonapoptotic cell death were discovered after the central mechanism of apoptosis was defined. Because apoptosis-deficient embryos develop nearly normally until birth, nonapoptotic cell death must play crucial roles in their development and may compensate for apoptosis in apoptosis-deficient mice or play an intrinsic role that is required for normal development.
Autophagy-dependent death plays a role in the development of the larval salivary glands of Drosophila.4, 10 Although a similar type of machinery is expected to exist in other organisms, to date, definitive evidence to suggest the existence of autophagy-dependent cell death in vivo in mammals is lacking. For example, Atg5 and Atg7 KO mouse embryos do not show detectable abnormalities during development. However, the Atg5/Atg7-independent autophagy pathway that we recently discovered7 may compensate for the developmental abnormalities caused by Atg5/Atg7 deficiency. Alternatively, deficiency of autophagy-dependent death might be compensated by apoptosis. Likewise, autophagic cell death may compensate for a deficiency or dysregulation of apoptosis. Therefore, developmental abnormalities might become apparent only in organisms deficient for both apoptosis and autophagy. Therefore, in this study, we evaluated this possibility and show that Atg5 deficiency caused embryonic death and enhanced developmental abnormalities, such as excencephaly and a delay in the elimination of interdegital webs, on an apoptosis-resistant background. Among these phenotypes, interdigital web clearance requires cell loss, and hence the induction of Atg5-dependent death should be the cause of the different phenotypes observed in DKO and TKO embryos. Regarding exencephaly, this occurs by a failure of neural tube closure in early stage embryos, and not only cell death-related genes but also genes governing proliferation, differentiation, and even actin regulation, are committed to this phenotype. However, of the various types of exencephaly, forebrain protrusion is only observed in cell death-related gene mutants,27, 28 and hence Atg5-dependent death might also be involved in the exencephaly (forebrain protrusion) in TKO embryos. These findings highlight a crucial role for autophagy-dependent death in mice, at least under conditions of apoptosis deficiency. Although recent findings show that necroptosis-deficient Rip3 KO and Mlkl KO mice do not exhibit detectable developmental abnormalities,29, 30 necroptosis might be involved in the compensation of apoptosis deficiency.
How do cells die by autophagic cell death? The detailed molecular mechanisms are unknown; however, our ultrastructural analysis provides some insights. First, there is a robust increase in the number and size of autophagic vacuoles in cells undergoing autophagic cell death compared with those undergoing starvation-induced autophagy. The diameter of starvation-induced autophagic vacuoles is mostly <1 μm, whereas autophagic vacuoles greater than 3 μm in diameter are frequently observed during autophagic cell death. Therefore, the hyperactivation of autophagy may be crucial for the induction of autophagic cell death. Ultrastructural and functional analyses of autophagic cell death indicate that most organelles, including mitochondria and the ER, appear normal. Therefore, it is unlikely that the degradation of specific organelles initiates autophagic cell death. Rather, we consider that the excessive degradation of cytosolic components by autophagy may represent the direct cause of autophagic cell death.
Taken together, the data presented here suggest an important role for autophagic cell death during the development of apoptosis-deficient mice. The role of this process in physiological contexts remains to be determined.
Materials and Methods
Generation of mutant mice
Bax+/−Bak−/− mice were crossed with C57BL/6 mice for at least 10 generations. Atg5/Bax/Bak TKO mice were generated by breeding Bax+/−Bak−/− mice with C57BL/6 Atg5+/− mice. The C57BL/6 background (>95%) was confirmed using 100 microsatellite markers. All mice were bred at the Laboratory for Recombinant Animals, Medical Research Institute, Tokyo Medical and Dental University; the Animal Facility of Osaka University Medical School; and the Animal Facility of Oriental Bioservice, Inc.
Antibodies and chemicals
An anti-Atg5 polyclonal antibody (Sigma-Aldrich Inc., St. Louis, MO, USA) was used for immunoblot assays. An anti-laminA/C (Cell Signaling Technology Inc., Danvers, MA, USA) monoclonal antibody was used for immunofluorescence assays. Anti-LC3 (NanoTools Inc.) and anti-GAPDH (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) monoclonal antibodies were used for immunoblot assays. The chemicals 3-MA, z-VAD-fmk, and DAPI were obtained from Sigma-Aldrich, Peptide Inc. (Ibaraki, Osaka, Japan), and Molecular Probes (Eugene, OR, USA), respectively. TUNEL Assay Kit was purchased from Chemicon International Co (Temecula, CA, USA). All other chemicals were purchased from Nacalai Tesque.
Cell culture and transfection
WT, Atg5−/−, Bax−/−Bak−/− (DKO), and Atg5−/−Bax−/−Bak−/− (TKO) MEFs were harvested from mouse embryos on E14.5 and immortalized with SV40 T antigen.12 MEFs that were passaged in the same way for over 15 passages were used. The passage number did not affect the cellular phenotype. Bak KO, DKO, and TKO primary thymocytes were harvested from the respective embryos on E18.5, as described previously.21 MEFs and thymocytes were cultured in Dulbecco’s modified Eagle’s medium supplemented with 2 mM L-glutamine, 1 mM sodium pyruvate, 0.1 mM nonessential amino acids, 10 mM HEPES/Na+ (pH 7.4), 0.05 mM 2-mercaptoethanol, 100 U/ml penicillin, 100 μg/ml streptomycin, and 10% fetal bovine serum.
In some experiments, MEFs (1 × 106) were transfected with GFP-LC3 plasmid DNA using the Amaxa electroporation system according to the supplier’s protocol (Kit V, Program U-20). The transfection efficiency was >75%, as assessed by GFP-fluorescent cells. MEFs (1 × 106) were also transfected with 10 μg of siRNA using RNAiMax (Thermo Co.) according to the supplier’s protocol. The siRNA sequences used were as follows: mouse Beclin-1 5′-UUGGUUUGGAAAGAUGCUUUA-3′, mouse Atg5 5′-GAGUCAGCUAUUUGACGUU-3′, and control siRNA (Dharmacon siGENOME Non-Targeting siRNA).
Staining of autophagosomes
Formation of LC3 puncta was examined using MEFs transiently transfected with 1 μg of the GFP-LC3 expression plasmid.31 Cells were treated with etoposide for 24 h after transfection, and GFP-LC3 fluorescence was observed using a confocal fluorescence microscope (LSM 510 META, Zeiss). Mouse tissues were fixed in 4% paraformaldehyde in PBS for 4 h and embedded in paraffin for the preparation of 2-μm sections and were subjected to TUNEL staining according to the supplier’s protocol. Immunofluorescence analysis was also performed using an anti-LC3 antibody.
Western blot analysis
Whole-cell protein extracts were prepared using RIPA buffer, and protein concentration was quantified using the Lowry method (Bio-Rad, Hercules, CA, USA) according to the manufacturer’s protocol. Proteins were separated by electrophoresis through 15% SDS-polyacrylamide gels and transferred to PVDF membranes. The membranes were incubated with the antibodies indicated in the figures, and the immune complexes were detected using the appropriate HRP-conjugated secondary antibodies and ECL reagent (Amersham, Little Chalfont, UK).
Assay of long-lived protein degradation
Degradation of long-lived proteins was measured as described previously.7 Briefly, cells were labeled for 20 h with a medium containing 0.2 μCi/ml L-[14C] valine (GE Healthcare Inc.). After washing and incubation in a medium containing 10 mM cold valine for 60 min, the medium was replaced with fresh medium and further incubated for the indicated times. Macromolecules in the medium were then precipitated using 10% TCA, and TCA-soluble radioactivity was measured. [14C]-valine release was calculated as a percentage of the total radioactivity in the TCA-soluble supernatant. The extent of protein degradation was calculated by subtracting the [14C]-valine release by untreated cells from that by starved or etoposide-treated cells.
Cell viability assay
MEFs were seeded onto 6-well dishes at 3.5 × 106 cells per well. After 24 h, the cells were exposed to etoposide (20 μM) or STS (1 μM) in the presence or absence of 3-MA (10 mM) or z-VAD-fmk (100 μM). After the observation of cells using phase-contrast microscopy, their viability was assessed using the CTB assay to measure the reduction of resazurin, which measures the metabolic activity of viable cells.12 Briefly, 5 × 104 cells were harvested, and the CTB assay reagent was added to the cells according to the supplier’s protocol. The extent of dead cells was calculated as the percentage relative to the initial value at each sampling time. To measure cell proliferation, MEFs were treated with etoposide, all cells were recovered, and 5 × 103 cells were cultured again in standard medium in 48-well dishes. Viable cells were measured on the indicated days using the CTB assay.12 For the colony formation assay, MEFs were treated with etoposide, harvested, and 2 × 103 cells were seeded onto 24-well dishes containing standard medium. After 1 week, cells were stained with calcein-AM, and images were acquired using fluorescence microscopy. Primary thymocytes were seeded onto 6-well dishes at 3.5 × 105 cells per well. After 24 h, the cells were exposed to STS (0.5 μM), etoposide (1 μM), or an anti-CD3 antibody (1 mg/ml) in the presence or absence of 3-MA (10 mM) or z-VAD-fmk (100 μM). Cells were harvested, and their viability was determined using PI staining.
Staining of the nuclear membrane
Cells were fixed in 4% formaldehyde, permeabilized in 0.1% Triton X-100, stained with an anti-laminA/C antibody, and incubated with an Alexa488-conjugated secondary antibody. DNA was counterstained with DAPI.
Electron microscopy
Cells were fixed with 1.5% paraformaldehyde/3% glutaraldehyde in 0.1 M phosphate buffer (pH 7.2) followed by fixation in 1% OsO4. After dehydration, ultrathin sections were stained with uranyl acetate and lead citrate and observed using a JEM-1010 electron microscope (JEOL Co. Ltd.) at 80 kV.
Analysis of interdigital webs
We crossed Atg5+/−Bax+/−Bak−/− mice to each other, and examined only a set of Bak KO, Bax/Bak DKO, and Atg5/Bax/Bak TKO littermates for the analysis of interdigital web elimination. An embryo was judged as having a web when a web was clearly present in at least one leg. The percentage of the eliminated interdigital web area was calculated by dividing the area surrounded by the proximal points of the thumb and little finger and all of the fingertips by the area of elimination of the interdigital web. Representative calculations are shown in Supplementary Fig. 7.
Statistical analysis
Results are expressed as the mean±S.D. Statistical evaluation was performed using Prism (GraphPad) software. Comparisons of multiple data sets were performed using one-way ANOVA followed by the Tukey post hoc test. A P-value of <0.05 was considered to indicate a statistically significant difference between two groups. Statistical analyses of non-random associations between two categorical variables were examined using the Fisher exact test.
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Acknowledgements
We are grateful to Dr N. Mizushima for providing us with Atg5+/− mice. This study was supported in part by Grant-in-Aid for Grant-in-Aid for Scientific Research (S) (22229002), Grant-in-Aid for Scientific Research (A) (17H01533), Grant-in-Aid for challenging Exploratory Research (16K15230), Grant-in-Aid for Scientific Research on Innovative Areas (15H01554, 26110001, 26110005), Grant-in-Aid for Encouragement of Young Scientists (B) (15K19004) from the MEXT of Japan, by the Project for Cancer Research and Therapeutic Evolution (P-CREATE) and by the Project for Psychiatric and Neurological Disorders from the Japan Agency for Medical Research and development, AMED. This study was also supported by the Joint Usage/Research Program of Medical Research Institute, Tokyo Medical and Dental University, and by a grant from the Secom Science and Technology Foundation and the Takeda Science Foundation.
Author contributions
SA performed EM analyses. MT, TY, HS, YN, YM, IY, and SS performed other experiments and analyzed the results. YT and SS designed and conducted the research, and wrote the paper.
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Arakawa, S., Tsujioka, M., Yoshida, T. et al. Role of Atg5-dependent cell death in the embryonic development of Bax/Bak double-knockout mice. Cell Death Differ 24, 1598–1608 (2017). https://doi.org/10.1038/cdd.2017.84
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DOI: https://doi.org/10.1038/cdd.2017.84
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