Abstract
Background/Objective:
One of the speculated mechanisms underlying fetal origin hypothesis of breast cancer is the possible influence of maternal environment on epigenetic regulation, such as changes in DNA methylation of the insulin-like growth factor-2 (IGF2) gene. The aim of the study is to investigate the relationship between folate, vitamin B12 and methylation of the IGF2 gene in maternal and cord blood.
Subjects/Methods:
We conducted a cross-sectional study to measure methylation patterns of IGF2 in promoters 2 (P2) and promoters 3 (P3).
Results:
The percentage of methylation in IGF2 P3 was higher in maternal blood than in cord blood (P<0.0001), whereas the methylation in P2 was higher in cord blood than in maternal blood (P=0.016). P3 methylation was correlated between maternal and cord blood (P<0.0001), but not P2 (P=0.06). The multivariate linear regression model showed that methylation patterns of both promoters in cord blood were not associated with serum folate levels in either cord or maternal blood, whereas the P3 methylation patterns were associated with serum levels of vitamin B12 in mother's blood (mean change (MC)=−0.22, P=0.0014). Methylation patterns in P2 of maternal blood were associated with serum levels of vitamin B12 in mother's blood (MC=−0.23, P=0.012), exposure to passive smoking (MC=0.46, P=0.034) and mother's weight gain during pregnancy (MC=0.23, P=0.019).
Conclusions:
The study suggests that environment influences methylation patterns in maternal blood, and then the maternal patterns influence the methylation status and levels of folate and vitamin B12 in cord blood.
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Acknowledgements
We thank all the participants in this study and all the doctors and nurses of Houzhai Center Hospital. This publication was made possible by CTSA Grant number UL1 RR024139 from the National Center for Research Resources (NCRR), a component of the NIH and NHL roadmap for medical Research. Its contents are solely the responsibility of the authors and do not necessarily represent the official view of NCRR. This research is also supported by Fogarty training Grants 1D43TW008323-01 and 1D43TW007864-01 from the NIH, and by the Grant 2009A330005 from the Education Department of Henan Province, China.
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Contributors: YB, HY, CZ, TZ, SM, and YZ designed the study; YB, HY, FL, XG, QZ, GW, ZL, and YZ collected the data; YB, HY, FL, GW, ZL, and YZ performed the analyses; YB and YZ wrote the first draft of the manuscript; and all authors reviewed and contributed to the final draft.
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Ba, Y., Yu, H., Liu, F. et al. Relationship of folate, vitamin B12 and methylation of insulin-like growth factor-II in maternal and cord blood. Eur J Clin Nutr 65, 480–485 (2011). https://doi.org/10.1038/ejcn.2010.294
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DOI: https://doi.org/10.1038/ejcn.2010.294
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