Summary
Deletion analysis of the episome from strain CB075 shows that the order and orientation of the structural genes for cysteine biosynthesis in Escherichia coli is
lysA thyA argA cysJIHDC
In Salmonella typhimurium the orientation of the corresponding genes was shown by a four-point conjugation cross to be
thyA argB cysC(DHI)J
Despite this inversion the structure of the gene clusters may be similar. If so, this is relevant to the study of the evolution of enteric bacteria. Complementation tests showed that E. coli gene formerly called cysP is functionally equivalent to the S. typhimurium gene cysJ.
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Jones-Mortimer, M. Mapping of structural genes for the enzymes of cysteine biosynthesis in Escherichia coli K12 and Salmonella typhimurium LT2. Heredity 31, 213–221 (1973). https://doi.org/10.1038/hdy.1973.76
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DOI: https://doi.org/10.1038/hdy.1973.76