Summary
Pollen from Nicotiana paniculata and the V12 variety of N. rustica was irradiated with a range of high doses of γ-rays up to 100 Krads. Both kinds of pollen were used to pollinate the V27 variety of N. rustica. Radiation treatments above 30 Krads gave no viable seed. A cytological examination of the M1 progeny from the 20 Krad treatments of both crosses revealed conventional radiation damage in the form of losses of whole chromosomes and parts of chromosomes, and rearrangements. The plants possessed hybrid or aberrantly hybrid phenotypes. It was concluded that they were the products of a conventional fertilisation mechanism rather than the gene transfer mechanism proposed by Pandey (1980). The expression of mutational damage can probably account for most of the maternal trends observed in the intervarietal M2 of N. rustica examined previously, although post-meiotic selection may also play a role.
Similar content being viewed by others
Article PDF
References
Caligari, P D S, Ingram, N R, and Jinks, J L. 1981. Gene transfer in Nicotiana rustica by means of irradiated pollen. I. Unselected progenies. Heredity, 47, 17–26.
Devreux, M, Donini, B, and Scarascia-Mugnozza, G T. 1972. Genetic effects of gametophyte irradiation in barley. II. Frequency of types of mutations induced. Radiation Botany, 12, 87–98.
Donini, B, Devreux, M, and Scarascia-Mugnozza, G T. 1970. Genetic effects of gametophyte irradiation in barley. I. Seedling mutants. Radiation Botany, 10, 79–86.
Engvild, K C. 1980. On gene transfer from radiation inactivated pollen of Nicotiana. Incompatibility Newsletter, 13, 111–113.
Gerstel, D U, Burns, J A, and Burk, L G. 1978. Cytoplasmic male sterility in Nicotiana, restoration of fertility, and the nucleolus. Genetics, 89, 157–169.
Goodspeed, T H. 1929. Cytological and other features of variant plants produced from X-rayed sex cells of N. tabacum. Botanical Gazette, 87, 565–582.
Ivanov, M A. 1938. Experimental production of haploids in N. rustica. Genetica, 20, 295–386.
Pandey, K K. 1975. Transformation, incompatibility and plant improvement. Incompatibility Newsletter, 6, 91–121.
Pandey, K K. 1978. Gametic gene transfer in Nicotiana by means of irradiated pollen. Genetica, 49, 53–69.
Pandey, K K. 1980. Further evidence for egg transformation in Nicotiana. Heredity, 45, 17–31.
Pandey, K K, and Phung, M. 1982. Hertwig effect in plants: induced parthenogenesis through the use of irradiated pollen. Theoretical and Applied Genetics, 62, 295–300.
Powell, W, Caligari, P D S, and Hayter, A M. 1983. The use of pollen irradiation in barley breeding. Theoretical and Applied Genetics, 65, 73–76.
Snape, J W, Parker, B B, Simpson, E, Ainsworth, C C, Payne, P I, and Law, C N. 1983. The use of irradiated pollen for differential gene transfer in wheat (Triticum aestivum). Theoretical and Applied Genetics, 65, 103–111.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Werner, C., Dunkin, I., Cornish, M. et al. Gene transfer in Nicotiana rustica by means of irradiated pollen II cytogenetical consequences. Heredity 52, 113–119 (1984). https://doi.org/10.1038/hdy.1984.11
Received:
Issue Date:
DOI: https://doi.org/10.1038/hdy.1984.11
This article is cited by
-
Single nucleus sequencing reveals spermatid chromosome fragmentation as a possible cause of maize haploid induction
Nature Communications (2017)
-
Pollination with irradiated pollen in rice—Oryza sativa L. I. First (M1) generation
Heredity (1989)
-
Pollination with irradiated pollen in rice—Oryza sativa L. II. The second (M2) generation
Heredity (1989)
-
Endosperm responses to irradiated pollen in apples
Theoretical and Applied Genetics (1987)
-
Gene transfer through the use of sublethally irradiated pollen: the theory of chromosome repair and possible implication of DNA repair enzymes
Heredity (1986)