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Somatic cell nuclear transfer

Nature volume 419, pages 583–587 (2002)Cite this article

Abstract

Cloning by nuclear transfer from adult somatic cells is a remarkable demonstration of developmental plasticity. When a nucleus is placed in oocyte cytoplasm, the changes in chromatin structure that govern differentiation can be reversed, and the nucleus can be made to control development to term.

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Figure 1: A comparison of survival to term of bovine embryos produced either by in vitro fertilization or nuclear transfer from embryos, fetal or adult fibroblasts.
Figure 2: 5-Methyl cytosine immunostaining of pronuclear DNA in mouse and sheep zygotes.

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Acknowledgements

We each acknowledge discussions with colleagues during the development of these ideas and are grateful for funding from the governments, charities and commercial agencies acknowledged in our research papers.

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Author notes

  1. D. N. Wells

    Present address: AgResearch Ltd, Hamilton, PB3123, New Zealand

  2. A. Dinnyes

    Present address: Research Group for Animal Breeding, Hungarian Academy of Sciences & Szent István University, Hungary

  3. L. E. Young

    Present address: School of Human Development, Division of Reproductive Medicine, Queens Medical Centre, Nottingham, NG7 2UH, UK

Authors and Affiliations

  1. Roslin Institute, Roslin, Midlothian, EH25 9PS, UK

    I. Wilmut, N. Beaujean, P. A. de Sousa, A. Dinnyes, T. J. King, L. A. Paterson & L. E. Young

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  1. I. Wilmut
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  2. N. Beaujean
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Correspondence to I. Wilmut.

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Wilmut, I., Beaujean, N., de Sousa, P. et al. Somatic cell nuclear transfer. Nature 419, 583–587 (2002). https://doi.org/10.1038/nature01079

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