Small interfering RNAs, synthesized using phage polymerases but not by chemical methods, can trigger the interferon response in mammalian cells.
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References
Kim, D-H. et al. Nat. Biotechnol. 22, 319–323 (2004).
Fire, A. et al. Nature 391, 806–811 (1998).
Samuel, C.E. Clin. Microbiol. Rev. 14, 778–809 (2001).
Alexopoulou, L., Holt, A.C., Medzhitov, R. & Flavell, R.A. Nature 413, 732–738 (2001).
Denli, A.M. & Hannon, G.J. Trends Biochem. Sci. 4, 196–201 (2003).
McManus, M.T. & Sharp, P.A. Nat. Rev. Genet. 3, 737–747 (2002).
Stewart, W.E. II The Interferon System (Springer, New York, 1979).
Marcus, P.I. Interferon, vol. 3 (ed. Gresser, I.) 115–180 (Academic Press, London, 1983).
Samuel, C.E. Proc. Natl. Acad. Sci. USA 76, 600–604 (1979).
Kerr, I.M. & Brown, R.E. Proc. Natl. Acad. Sci. USA 75, 256–260 (1978).
Sledz, C.A. et al. Nat. Cell Biol. 5, 834–839 (2003).
Bridge, A.J. et al. Nat. Genet. 34, 263–264 (2003).
Mellits, K.H. et al. Nucleic Acids Res. 18, 5401–5406 (1990).
Konarska, M.M. & Sharp, P.A. Cell 63, 609–618 (1990).
Persengiev, S.P., Zhu, X. & Green, M.R. RNA 10, 12–18 (2004).
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Samuel, C. Knockdown by RNAi—proceed with caution. Nat Biotechnol 22, 280–282 (2004). https://doi.org/10.1038/nbt0304-280
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DOI: https://doi.org/10.1038/nbt0304-280
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