Abstract
Freeze-dried mouse spermatozoa are all motionless and dead in the conventional sense. When injected into oocytes, however, their nuclei can support normal embryonic development even after three month preservation in a dried state. Although the freeze-drying protocol reported here will need further improvement, the results suggest that it may be possible to store the male genomes at room temperature.
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Wakayama, T., Yanagimachi, R. Development of normal mice from oocytes injected with freeze-dried spermatozoa. Nat Biotechnol 16, 639–641 (1998). https://doi.org/10.1038/nbt0798-639
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DOI: https://doi.org/10.1038/nbt0798-639
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