Abstract
We have recovered transgenic rice plants from a number of commercially important cultivars, including until now recalcitrant Indica varieties, using electric discharge particle acceleration. Immature embryos from greenhouse–grown plants were bombarded with gold particles carrying DNA, and transgenic plants were recovered following a simple culture protocol. Mendelian segregation of foreign genes was observed in R1 progeny and stable integration was demonstrated by Southern blot analysis of genomic DNA isolated from progeny plants. Alternative transformation protocols that are dependent on the development of protoplast and suspension culture systems are no longer necessary as we have shown that a wide variety of diverse cultivars can be transformed. Transgenic plants expressing agronomically useful traits such as herbicide resistance have been obtained and are currently undergoing further evaluation. This report also demonstrates that it is possible to produce transgenic monocoty–ledonous plants by transforming scutellar tissue of immature embryos.
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References
Gordon-Kamm, W.J., Spencer, T.M., Mangano, M.L., Adams, T.R., Daines, R.J., Start, W.G., O'Brien, J.V., Chambers, S.A., Adams, W.R., Willetts, N.G., Rice, T.B., Mackey, C.J., Krueger, R.W., Kausch, A.P. and Lemaux, P.G. 1990. Transformation of maize cells and regeneration of fertile transgenic plants. The Plant Cell 2: 603–618.
Umbeck, P., Johnson, G., Barton, K.A. and Swain, W.F. 1987. Genetically transformed cotton (Gossypium hirsutum L.) plants. Bio/Technology 5: 263–266.
McCabe, D.E., Swain, W.F., Martinell, B.J. and Christou, P. 1988. Stable transformation of soybean (Glycine max) by particle acceleration. Bio/Technology 6: 923–926.
Fromm, M.E., Morrish, F., Armstrong, C., Williams, R., Thomas, J. and Klein, T.M. 1990. Inheritance and expression of chimeric genes in the progeny of transgenic maize plants. Bio/Technology 8: 833–844.
Toriyama, K., Arimoto, Y., Uchimiya, H. and Hinata, K. 1988. Transgenic rice plants after direct gene transfer into protoplasts. Bio/Technology 6: 1072–1074.
Zhang, H.M., Yang, H., Rech, E.L., Golds, T.J., Davis, A.S., Mulligan, B.J. and Cocking, E.G. 1988. Transgenic rice plants produced by electroporation mediated plasmid uptake into protoplasts. Plant Cell Reports 7: 379–383.
Shimamoto, K., Teda, R., Izawa, T. and Fujimoto, H. 1989. Fertile transgenic rice plants regenerated from transformed protoplasts. Nature 338: 274–277.
Tada, Y., Sakamoto, M. and Fujimura, T. 1990. Efficient gene introduction into rice by electroporation and analysis of transgenic plants: use of electroporation buffer lacking chloride ions. Theor. Appl. Genet. 80: 475–480.
Hayashimoto, A., Li, Z. and Murai, N. 1990. A PEG-mediated protoplast transformation system for production of fertile transgenic rice plants.Plant Physiology 93: 857–863.
Datta, S.K., Peterhans, A., Datta, K. and Potrykus, I. 1990. Genetically engineered fertile Indica-rice recovered from protoplasts. Bio/Technology 8: 736–740.
Raineri, D.M., Bottino, P., Gordon, M.P. and Nester, E.W. 1990. Agrobacterium-mediated transformation of rice (Oryza sativa L.). Bio/Technology 8: 33–38.
Luo, Z. and Wu, R. 1988. A simple method for the transformation of rice via the pollen-tube pathway.Plant Mol. Biol. Rep. 6: 165–174.
Christou, P., McCabe, D.E., Martinell, B.J. and Swain, W.F. 1990. Soybean genetic engineering—Commercial production of transgenic plants. Trends in Biotechnology 8: 145–151.
Potrykus, I. 1989. Gene transfer to cereals: an assessment. Trends in Biotech. 7: 269–273.
Potrykus, I. 1990. Gene transfer to plants: assessment and perspectives. Physiol. Plantarum 79: 125–134.
Swaminathan, M.S. 1982. Biotechnology research and third world agriculture. Science 218: 967–972.
Wu, R., Kemmerer, E. and McElroy, D. 1990. Transformation and regeneration of important crop plants: Rice as the model system for monocots. Gene manipulation in plant improvement II: 251–263.
Potrykus, I. 1990. Gene transfer to cereals. An assessment. Bio/Technology 8: 535–542.
Peng, J., Lyznik, L.A., Lee, L. and Hodges, T.K. 1990. Cotransformation of indica rice protoplasts with gus A and neo genes. Plant Cell Reports 9: 168–172.
Christou, P., McCabe, D.E. and Swain, W.F. 1988. Stable transformation of soybean callus by DNA-coated gold particles. Plant Physiol. 87: 671–674.
Hartke, S. and Lorz, H. 1989. Somatic embryogenesis and plant regeneration from various indica rice (Oryza sativa L.) genotypes. J. Genet. & Breed. 43: 205–214.
Datta, S.K., Datta, K. and Potrykus, I. 1990. Embryogenesis and plant regeneration from microspores of both Indica and Japonica rice (Oryza sativa). Plant Sci. 67: 83–88.
Dekeyser, R., Claes, B., Marichal, M., Van Montagu, M. and Caplan, A. 1989. Evaluation of selectable markers for rice transformation. Plant. Physiol. 90: 217–223.
Christou, P., Swain, W.F., Yang, N.S. and McCabe, D.E. 1989. Inheritance and expression of foreign genes in transgenic soybean plants. Proc. Natl. Acad. Sci. USA 86: 7500–7504.
Christou, P. and Swain, W.F. 1990. Cotransformation frequencies of foreign genes in soybean cell cultures. Theor. Appl. Genet. 79: 337–341.
Jefferson, R.A., Kavanagh, T.A. and Bevan, M.W. 1987. GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.EMBO J. 6: 3901–3907.
De Block, M., Botterman, J., Vandewiele, M., Dockx, J., Thoen, C., Gossele, V., Rao Movva, N., Thompson, C., Van Montagu, M. and Leemans, J. 1987. Engineering herbicide resistance in plants by expression of a detoxifying enzyme. EMBO J. 6: 2513–2518.
Berry-Lowe, S., McKnight, T.D., Shah, D.M. and Meagher, R.B. 1982. The nucleotide sequence, expression, and evolution of one member of a multigene family encoding the small subunit of ribulose-1,5-bisphosphate carboxylase in soybean. J. Mol. Appl. Genet. 1: 483–498.
Dellaporta, S.L., Wood, J. and Hicks, J.B. 1984. Maize DNA mini-prep, p. 36–37. In: Molecular Biology of Plants. A Laboratory Course Manual R. Malmberg, J. Messing and I. Sussex (Eds.) Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
Southern, E.M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98: 503–517.
Church, G.M. and Gilbert, W. 1984. Genomic sequencing. Proc. Natl. Acad. Sci. USA. 81: 1991–1995.
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Christou, P., Ford, T. & Kofron, M. Production of Transgenic Rice (Oryza Sativa L.) Plants from Agronomically Important Indica and Japonica Varieties via Electric Discharge Particle Acceleration of Exogenous DNA into Immature Zygotic Embryos. Nat Biotechnol 9, 957–962 (1991). https://doi.org/10.1038/nbt1091-957
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DOI: https://doi.org/10.1038/nbt1091-957
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