Abstract
We have increased the production of bovine chymosin in Aspergillus niger var. awamori to more than one gram per liter of secreted authentic enzyme by combining a mutagenesis protocol with a novel robotic screening program. Analysis of the superior chymosin producing strains indicated that they have enhanced capabilities to secrete extracellular proteins.
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Dunn-Coleman, N., Bloebaum, P., Berka, R. et al. Commercial Levels of Chymosin Production by Aspergillus. Nat Biotechnol 9, 976–981 (1991). https://doi.org/10.1038/nbt1091-976
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DOI: https://doi.org/10.1038/nbt1091-976
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