Abstract
Organelles called centrosomes in metazoans or spindle pole bodies (SPBs) in yeast direct the assembly of a bipolar spindle that is essential for faithful segregation of chromosomes during mitosis. Abnormal accumulation of multiple centrosomes leads to genome instability, and has been observed in both tumour cells and cells with targeted mutations in tumour-suppressor genes. The defects that lead to centrosome amplification are not understood. We have recapitulated the multiple-centrosome phenotype in budding yeast by disrupting the activity of specific cyclin-dependent kinase (CDK) complexes. Our observations are reminiscent of mechanisms that govern DNA replication, and show that specific cyclin/CDK activities function both to promote SPB duplication and to prevent SPB reduplication.
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Acknowledgements
We thank I. Adams for the Spc42–GFP construct pIA29, and J. Kilmartin for initial electron-microscopic observations. We also thank M. Watson, N. Rhind, T. T. Su and M. Morris for critical reviewing of the manuscript, M. Wolff for technical assistance, and M. Wood, A. Sanusi and T. H. Giddings Jr for electron-microscopic work. This work was supported by NIH grant nos GM38328 (to S.I.R.) and GM51312 (to M.W).
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Haase, S., Winey, M. & Reed, S. Multi-step control of spindle pole body duplication by cyclin-dependent kinase. Nat Cell Biol 3, 38–42 (2001). https://doi.org/10.1038/35050543
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DOI: https://doi.org/10.1038/35050543
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