Abstract
During physiological T-cell stimulation by antigen presenting cells (APCs), a major T-cell membrane rearrangement is known to occur leading to the organization of 'supramolecular activation clusters' at the immunological synapse1,2. A possible role for the synapse is the generation of membrane compartments where signalling may be organized and propagated2. Thus, engagement of the costimulatory molecule CD28 at the immunological synapse promotes the organization of a signalling compartment by inducing cytoskeletal changes and lipid raft accumulation3,4,5. We identified the actin-binding protein Filamin-A (FLNa) as a novel molecular partner of CD28. We found that, after physiological stimulation, CD28 associated with and recruited FLNa into the immunological synapse, where FLNa organized CD28 signalling. FLNa knockdown by short interfering RNA (siRNA) inhibited CD28-mediated raft accumulation at the immunological synapse and T-cell costimulation. Together, our data indicate that CD28 binding to FLNa is required to induce the T-cell cytoskeletal rearrangements leading to recruitment of lipid microdomains and signalling mediators into the immunological synapse.
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Acknowledgements
We thank T. P. Stossel and F. Nakamura for providing FLNa constructs and advice, and T. Harder for scientific discussion. We also thank M. Bettella and A. Cabrelle for technical support. This work has been supported by grants from the Italian Association for Cancer Research (AIRC) and University of Padua (Progetto d'Ateneo) to A.V. and from AIRC, the Italian Space Agency (ASI, MoMa “From Molecule to Man” Project) to L.T. L.T is also supported by “Fondazione Andrea Cesalpino” Policlinico Umberto I (University of Rome “La Sapienza”, Rome Italy). S.M. is supported by the Spanish Ministry of Education and Science (SAF2005-00241).
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A.V. designed the study and wrote the manuscript. S.M. provided reagents and designed the yeast two-hybrids experiment. L.T. provided reagents and suggestions. M.S. performed the NF-AT luciferase assay and control experiments not shown in the manuscript. S.J.B. performed the yeast two-hybrid assay. R.T. and R.L.C. performed all the other experiments, prepared the figures and performed statistical tests.
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Tavano, R., Contento, R., Baranda, S. et al. CD28 interaction with filamin-A controls lipid raft accumulation at the T-cell immunological synapse. Nat Cell Biol 8, 1270–1276 (2006). https://doi.org/10.1038/ncb1492
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DOI: https://doi.org/10.1038/ncb1492
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