Abstract
We developed a general method to detect cellular small molecule–RNA conjugates that does not rely on the reactivity of the small molecule. This technique revealed NAD-linked RNA in Escherichia coli and Streptomyces venezuelae. Subsequent characterization showed that NAD is a 5′ modification of RNA, cannot be installed in vitro through aberrant transcriptional initiation, is only found among smaller cellular RNAs and is present at a surprisingly high abundance of ∼3,000 copies per cell.
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Acknowledgements
This work was supported by the Howard Hughes Medical Institute and the US National Institute of General Medical Sciences, National Institutes of Health (R01GM065865). Y.G.C. and W.E.K. gratefully acknowledge US National Science Foundation Graduate Research Fellowships.
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Y.G.C., W.E.K. and D.R.L. designed research; Y.G.C., W.E.K., I.A. and Y.S. performed research; Y.G.C., W.E.K. and I.A. contributed new reagents and analytic tools; Y.G.C., W.E.K., I.A., Y.S. and D.R.L. analyzed data; and Y.G.C., W.E.K., Y.S. and D.R.L. wrote the paper.
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Chen, Y., Kowtoniuk, W., Agarwal, I. et al. LC/MS analysis of cellular RNA reveals NAD-linked RNA. Nat Chem Biol 5, 879–881 (2009). https://doi.org/10.1038/nchembio.235
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DOI: https://doi.org/10.1038/nchembio.235
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