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Ligand binding modes from low resolution GPCR models and mutagenesis: chicken bitter taste receptor as a test-case
Scientific Reports Open Access 15 August 2017
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Acknowledgements
We thank E. Shippey and A. Klugo for help in preparing the manuscript and the Wadsworth Center Molecular Genetics Core Laboratory for oligonucleotide synthesis for PCR. This work was supported in part by grants from the National Institutes of Health (RO1AI33334 and U01AI35004 from the National Institute of Allergy and Infectious Diseases and the National Institute on Drug Abuse).
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Fang, G., Weiser, B., Visosky, A. et al. PCR-mediated recombination: A general method applied to construct chimeric infectious molecular clones of plasma-derived HIV–1 RNA. Nat Med 5, 239–242 (1999). https://doi.org/10.1038/5607
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DOI: https://doi.org/10.1038/5607
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