Abstract
We found that the combination of spatially restricted uracil phosphoribosyltransferase (UPRT) expression with 4-thiouracil delivery can be used to label and purify cell type–specific RNA from intact complex tissues in Drosophila melanogaster. This method is useful for isolating RNA from cell types that are difficult to isolate by dissection or dissociation methods and should work in many organisms, including mammals and other vertebrates.
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Acknowledgements
We thank C. Canestro, T. Herman, Z. Lewis and S. O'Rourke for comments on the manuscript, and C. Cabernard for help with Imaris. M.R.M. was supported by a US National Science Foundation predoctoral fellowship, M.D.C. was supported by a National Institutes of Health National Research Service Award postdoctoral fellowship, and C.Q.D. was supported by National Institutes of Health HD27056 and the Howard Hughes Medical Institute.
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Miller, M., Robinson, K., Cleary, M. et al. TU-tagging: cell type–specific RNA isolation from intact complex tissues. Nat Methods 6, 439–441 (2009). https://doi.org/10.1038/nmeth.1329
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DOI: https://doi.org/10.1038/nmeth.1329
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